Agilent Acea xCELLigence RTCA ePacer Cardiomyocyte Pacing and Real-Time Cell Analysis System

The Agilent Acea xCELLigence RTCA ePacer Cardiomyocyte Pacing System is an advanced platform designed for precise electrical stimulation and real-time impedance monitoring of cardiomyocyte cultures. This system supports *high-throughput cardiac electrophysiological assays* critical in cardiac safety screening, drug toxicity testing, and stem cell–derived cardiomyocyte maturation.

Key technical specifications include:

• Impedance measurement rate at 250 Hz providing high-resolution cell activity data with ±(1.5% + 1 Ω) accuracy and 0.8% repeatability.
• Stimulation pulse parameters: bipolar rectangular, triangle, sine, trapezoid, and exponential decay waveforms, adjustable pulse voltage from -2.5 V to +2.5 V, pulse width 0.02–600 ms, and frequency range 0.01–125 Hz facilitating physiological pacing.
• Supports scalable multi-well formats including 3 Cardio 96-well and 3 CardioECR 48-well E-Plates, with volumes of approximately 263–264 μL per well and optimized sensor impedance for accurate biophysical cell impedance readings.
• Integrated RTCA Cardio Temperature Tool ensures assay stability by maintaining precise temperature control (15–40°C) and minimizing environmental variability during live-cell cardiac assays.
• Electrical interface designed to minimize noise with switch resistances ranging 4–8 Ω and sensor impedances calibrated for standard PBS solutions, promoting signal fidelity essential for *biotechnology applications such as molecular diagnostics and bioprocessing research*.

This system is widely adopted in biomedical engineering and synthetic biology research, enabling researchers to synchronously pace hiPSC-derived cardiomyocytes and improve maturation timelines within 2–3 weeks. Its capability for dynamic electrophysiological pacing at high temporal resolution makes it a critical instrument in *biopharmaceutical production pipelines* for cardiotoxicity assessment and novel drug screening workflows.

The Agilent Acea xCELLigence RTCA ePacer combines hardware robustness with software flexibility (RTCA software license required from Agilent) to deliver reproducible, scalable cardiac assays essential for live-cell imaging, fluorescence analysis, and electrophysiological monitoring in biotechnology and clinical diagnostics fields.

Primary keywords: cardiomyocyte pacing system, real-time cell analyzer, impedance-based cardiac assay, electrophysiological pacing, temperature-controlled cardiac analysis Long-tail keywords: essential for molecular cloning workflows, widely adopted in synthetic biology research, critical in biopharmaceutical production pipelines, widely used in neuroimaging applications, popular among molecular biology researchers

The system ships complete with a computer running a trial version of the RTCA software, communication and power cables, and multiple Cardio and CardioECR cradles to support flexible experimental designs. It undergoes rigorous onsite functional testing including PBS solution resistance calibration, system diagnostic checks, and software connectivity verification to ensure full operational functionality and reliability before deployment in research settings.

Ideal for researchers in *cell imaging, cardiac safety pharmacology, and stem cell electrophysiology*, this system supports advanced biotechnology workflows by integrating precise electrical pacing with real-time cellular impedance monitoring under tightly regulated assay conditions.