Bio-Rad QX200 Droplet Digital PCR System with AutoDG Automated Droplet Generator, PX1 Plate Sealer, and C1000 Touch Thermal Cycler

The Bio-Rad QX200 Droplet Digital PCR system is a high-sensitivity molecular diagnostics platform designed for precise nucleic acid quantification using droplet digital PCR technology, essential for applications in biotechnology and clinical diagnostics such as gene expression analysis, copy number variation detection, and rare mutation identification.

This integrated system comprises:

• AutoDG Automated Droplet Generator: Generates approximately 20,000 droplets per 20 µL sample, capable of processing 1–96 samples per run, improving droplet generation reproducibility and throughput for bioprocessing and gene editing workflows.
• QX200 Droplet Reader: Features a multipixel photon counter for accurate fluorescence detection across FAM (EvaGreen) and HEX (VIC) channels; supports a linear dynamic range spanning five orders of magnitude and analyzes ~1.5 million droplets per 96-well plate, critical for molecular cloning and synthetic biology research.
• C1000 Touch Thermal Cycler: Equipped with a 96-deep well reaction module for versatile PCR formats (0.2 mL tubes, 0.5 mL tubes, or 96-well plates); offers precise thermal control (±0.2°C accuracy), a maximum ramp rate of 2.5°C/sec, and customizable programming, facilitating robust amplification protocols in biomanufacturing and molecular diagnostics applications.
• PX1 Plate Sealer: Provides rapid plate sealing (under 3 minutes) over a temperature range of 100–190°C, ensuring sample integrity and preventing evaporation or contamination during thermal cycling.

The system includes dedicated software and a computer interface for streamlined data acquisition and analysis, widely adopted in synthetic biology research and essential for quantitative viral load measurements and pathogen detection. This configuration enhances laboratory productivity with automated workflows and high-throughput capabilities, making it an indispensable instrument in advanced biotechnology and biomedical engineering laboratories.

Key technical features:

• Droplet generation capacity: up to 96 samples/run, ~20,000 droplets/sample
• Detection channels: FAM (EvaGreen), HEX (VIC) for multiplex digital PCR assays
• Thermal cycler capabilities: 0–100°C temperature range, 2.5°C/sec max ramp rate
• High precision: ±10% droplet reader precision, ±0.2°C temperature accuracy
• Automated plate sealing with precise temperature and time control

This system is critical for applications requiring highly sensitive nucleic acid quantification such as gene expression profiling, copy number variation detection, and rare mutation analysis, supporting advanced research and biopharmaceutical production pipelines. The Bio-Rad QX200 model is a predecessor to the QX600 and continues to be supported with service, replacement parts, and consumables, making it a reliable choice for molecular biology researchers and biotechnologists.