Olympus BX53 Upright Fluorescence Phase Contrast Microscope with New Fluorescence Filters

The Olympus BX53 Upright Fluorescence Phase Contrast Microscope is a versatile benchtop microscopy system designed for advanced fluorescence imaging and phase contrast applications, widely used in biotechnology, clinical diagnostics, and biomedical research. This microscope employs infinity-corrected optics to deliver high-resolution, distortion-free imaging, critical for precise cellular and molecular analysis in bioprocessing, gene editing, and molecular diagnostics workflows.

Key features include:

• Fluorescence illumination via an EXFO X-Cite 120W Metal Halide lamp with a collimating adapter and liquid light guide for uniform excitation intensity at the specimen plane.
• 8-position mechanical filter cube turret equipped with three brand new fluorescence filter sets selectable from DAPI, FITC/EGFP, Texas Red, TRITC/Cy3, and Cy5 (optional upgrade required), enabling multiplex fluorescence assays essential in synthetic biology and fluorescence analysis.
• Phase contrast capabilities supported by a U-PCD mechanical condenser with PH1 annulus for enhanced visualization of transparent live cells without staining, integral to live-cell imaging and biotechnology research.
• Trinocular head with dual eyepieces and configurable camera ports (100% eyes, 50/50 split, or 100% camera) for seamless integration of CCD, sCMOS, or EMCCD cameras, facilitating image capture, annotation, and quantitative analysis using ImageJ and proprietary capture software.
• A mechanical 6-position objective turret supporting standard Olympus UPlanFL and phase contrast objectives (4x, 10x, 20x), with options for high-end Apochromat objectives to increase magnification up to 400x, suitable for detailed cellular studies and biopharmaceutical material characterization.
• Mechanical XY stage for precise sample positioning, compatible with slides, Petri dishes, and microplates, enabling automated scanning for multi-sample workflows relevant in molecular cloning and biomanufacturing processes.

Additional benefits:

• Preventive maintenance completed with verified fluorescence intensity, optical uniformity, and mechanical integrity.
• Flexible modular design supports addition of DIC, polarization, motorized stages, shutters, and filter turrets to tailor the system for complex fluorescence and phase contrast protocols.
• Included remote training and technical consultation support effective adoption in research settings.
• Covered by a 180-day continental USA warranty and 60 days international warranty, ensuring reliability and post-sale support for research professionals.

This microscope’s configuration and extensive fluorescence filter options make it highly suitable for applications in neuroscience, cell imaging, biotechnology, pharmaceutical development, and molecular diagnostics. It is essential for molecular cloning workflows and widely adopted in synthetic biology research due to its robust fluorescence imaging and phase contrast features.

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This system is a preferred choice among university, pharmaceutical, and biotechnology laboratories for its adaptability, optical precision, and comprehensive support for fluorescence and phase contrast microscopy applications.