Thermo Fisher Qubit 4 Fluorometer for DNA, RNA, and Protein Quantification

The Thermo Fisher Qubit 4 Fluorometer is a high-precision, benchtop fluorescence detection device optimized for rapid quantification of nucleic acids and proteins crucial in molecular diagnostics and bioprocessing workflows. Featuring a 5.7-inch intuitive touchscreen and a dual-core processor, it enables efficient assay navigation, customization, and multilingual operation.

Key technical specifications include:

• Sample throughput: ~3 seconds per sample with results in under 5 seconds
• Sample volume: Requires only 1 µL, minimizing reagent costs and preserving precious samples
• Light sources: Blue LED (470 nm) and Red LED (635 nm) for excitation
• Excitation filters: 430–495 nm (Blue), 600–645 nm (Red)
• Emission filters: Green (510–580 nm), Red (665–720 nm) enhancing dye-based fluorescence detection
• Quantification targets: Sensitive measurement of DNA, RNA, and protein concentrations, delivering accuracy superior to UV absorbance methods especially at low concentrations
• Data management: Storage capacity of 1,000 results with export options including Wi-Fi, USB, direct USB connection, and Ethernet, supporting streamlined laboratory data workflows
• Programmability: Supports MyQubit programmable assays and includes reagent calculator for protocol optimization
• System verification: Compatible with Qubit 4 System Verification Assay Kit to maintain instrument performance integrity
• Physical dimensions: Compact (6 x 10 x 3 inches), lightweight (5 lbs) design ideal for benchtop use in biotechnology and molecular biology labs
• Power requirements: 120 V

This fluorometer uses a mix-and-read assay format with short incubation times—2 minutes for nucleic acid assays and up to 15 minutes for protein—making it essential for time-sensitive molecular cloning and synthetic biology projects. The instrument’s fluorometer mode and programmable features make it adaptable for various fluorescent assays used in gene editing, biomanufacturing, and molecular diagnostics.

Designed for researchers, clinical diagnostic labs, and biotechnologists, the Qubit 4 Fluorometer provides reliable, high-sensitivity quantification, critical in biopharmaceutical production pipelines and other advanced biotechnology applications.