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Agilent 8453 Diode Array UV-Vis Spectrophotometer System for Molecular Diagnostics

UsedAgilentOptical detectorsUnited States

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Wavelength range
190–1100 nm
Slit width
1 nm
EP resolution test
> 1.6 toluene in hexane, ratio abs. at 269 nm/266 nm
Stray light
< 0.03 % at 340 nm (NaNO2, ASTM) < 0.05 % at 220 nm (NaI, ASTM) < 1 % at 198 nm (KCl, EP)
Wavelength accuracy
< ± 0.5 nm 0.5-second scan (NIST 2034) < ± 0.2 nm at 486.0 and 656.1 nm
All 18 specifications
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Specifications 18

Brands
Agilent
New/Used
Used
Wavelength range
190–1100 nm
Slit width
1 nm
EP resolution test
> 1.6 toluene in hexane, ratio abs. at 269 nm/266 nm
Stray light
< 0.03 % at 340 nm (NaNO2, ASTM) < 0.05 % at 220 nm (NaI, ASTM) < 1 % at 198 nm (KCl, EP)
Wavelength accuracy
< ± 0.5 nm 0.5-second scan (NIST 2034) < ± 0.2 nm at 486.0 and 656.1 nm
Wavelength reproducibility
< ± 0.02 nm ten consecutive scans (NIST 2034)
Photometric accuracy
< ± 0.005 A at 440.0, 465.0, 546.1, 590.0, and 635.0 nm, 1 A (NIST 930e) < ± 0.01 A at 235, 257, 313, 350, 430 nm, (potassium dichromate, EP method)
Photometric noise
< 0.0002 A sixty 0.5 second scans at 0 A, 500 nm, rms
Photometric stability
< 0.001 A/h at 0 A, 340 nm, after 1-hour warm up, measured over 1 hour, every 5 seconds, constant ambient temp.
Baseline flatness
< 0.001 A 0.5-second blank, 0.5-second scan, rms
Typical scan time
1.5 second full range
Shortest scan time
0.1 second full range
Time until next scan
0.1 second full range, 0.1-second scan, up to 150 consecutive scans
Dimensions
24x16x8”
Weight
50lbs
Power
100-120/220-240V, 50/60 Hz

Product description

Fast, full-range 190–1100 nm Agilent 8453 diode array UV-Vis spectrophotometer with 1 nm slit and low noise.

The Agilent 8453 Spectrophotometer is a diode array UV-Vis spectrophotometer designed for precise and rapid spectral measurement across the 190–1100 nm range. This *single-beam system* utilizes stable optics with no moving parts to deliver high throughput and low noise, essential for reproducible results in molecular diagnostics and bioprocessing research.

Key technical specifications include:

  • Wavelength range: 190–1100 nm, supporting UV to visible light applications
  • Slit width: 1 nm, minimizing stray light to less than 0.03%
  • Wavelength accuracy: ±0.5 nm (0.5-second scan), with reproducibility under ±0.02 nm for ten consecutive scans
  • Photometric accuracy: better than ±0.005 A at key wavelengths (440.0–635.0 nm)
  • Photometric noise: less than 0.0002 A (rms) at 500 nm, ensuring high sensitivity for low-absorbance measurements
  • Baseline flatness and stability: under 0.001 A, meeting stringent analytical performance criteria
  • Scan speed: typical full-range scan time of 1.5 s, with shortest scans down to 0.1 s, enabling high-throughput screening
  • Physical dimensions: compact 24×16×8 inches footprint, weighing approximately 50 lbs for benchtop use

The instrument supports compliance with GLP through integrated clock and self-test procedures, safeguarding traceability and performance validation critical in biotechnology workflows such as gene editing assays, synthetic biology, and biopharmaceutical production pipelines.

The Agilent 8453 features an open sample compartment compatible with diverse accessories including single-cell holders, multi-cell transports, and sipper systems. Control and data acquisition are managed via Windows-based ChemStation software, facilitating complex data analysis with multiple parallel calculations and 21 mathematical functions—a valuable tool for researchers requiring advanced photometric evaluation in cell imaging, fluorescence analysis, and molecular diagnostics.

This spectrophotometer is widely adopted in biotechnology and clinical diagnostic laboratories for applications requiring rapid, accurate UV-Vis absorption measurements, making it an essential instrument in biochemical characterization and synthetic biology research.

Included with the system:

  • Agilent 8453 UV-Vis Spectrophotometer with diode array
  • Windows 10 Notebook running UV-Vis ChemStation software
  • System validation reports
  • New UV and visible lamps installed
  • Single-cell sample holder
  • Communication cable and power cord
  • User manual and 30-day warranty

Overall, the Agilent 8453 UV-Vis Spectrophotometer is a reliable, high-precision instrument optimized for bioprocessing, molecular cloning workflows, and widespread biotechnology applications requiring quality optical absorption data.

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Frequently asked questions

How long does laboratory equipment typically last?

With a service contract, ten to fifteen years is normal for most benchtop instruments. Detectors and pumps are the parts that wear first.

Can I get service for older equipment?

Often, yes. Verified suppliers frequently work with service partners converting the instrument. If applicable, we can list servicing options alongside the offer.

Where can I buy parts?

Ask a scientist through the request button. Parts are often available even when the instrument itself is discontinued.

What is the typical lead time for refurbished equipment?

Two to six weeks, depending on refurbishment scope and whether calibration is included.

Does laboratory equipment ship internationally?

We ship to 38 countries. Crating and customs handling are quoted with the instrument.

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Last updated 6 November 2026