Sartorius Biostat B Twin Bioreactor with 1L Jacketed Autoclavable Vessels and Integrated Control System

The Sartorius Biostat B Twin Bioreactor is a dual-vessel system designed for controlled *microbial fermentation* and bioprocess research in biotechnology, molecular diagnostics, and biomanufacturing pipelines. Each vessel features a 1-liter autoclavable double-wall jacketed glass vessel supporting robust temperature regulation via an external thermostat system, enabling precise culture environment control essential for microbial cultivation and media optimization studies.

Key system features include:

• Integrated twin control tower with touchscreen interface, controlling both vessels independently for parallel microbial fermentation experiments.
• Direct-drive agitation motors (200 W) with speeds ranging from 20 to 2000 rpm, ensuring efficient mixing and optimized oxygen transfer critical for aerobic bioprocess workflows.
• Precise gas delivery system with dual Thermal Mass Flow Controllers (TMFCs) for air and O₂, sparging up to 3 L/min, allowing fine control over dissolved oxygen levels in cultures.
• Multiple built-in peristaltic pumps (Watson-Marlow 114 series), fixed speed, for automated acid/base addition and substrate feeds, maintaining stable pH and nutrient supply during fed-batch processes.
• High-accuracy gravimetric feed and vessel scales enable real-time monitoring of substrate additions and biomass accumulation, facilitating advanced metabolic and bioprocess control studies.
• Extensive digital connectivity ports for sensor integration including pH/Redox (e.g., Mettler Toledo InPro), optical dissolved oxygen (e.g., VisiFerm DO), temperature (Pt100), and external process signals.

Additional technical specifications:

• Vessel working volume: 0.35 to 1 L (total volume 1.6 L)
• Jacketed vessel temperature control range: from 8 °C above coolant inlet temperature up to 80 °C
• Heating capacity: 600 W
• Dimensions: 35×82.2×43 cm; weight: 55 kg
• Power: 120 V, 60 Hz, max 12 A

Applications: This twin bioreactor is widely adopted in synthetic biology research, microbial strain screening, batch/fed-batch fermentation optimization, biopharmaceutical production pipelines, and microbial metabolic engineering. Its precise environmental and substrate control make it essential for molecular cloning workflows and experimental bioprocess development.

Customization options include alternative vessel sizes (up to 10 L), impeller types, external feed pumps, exhaust condensers, and temperature control accessories such as chillers and heated exhaust filters.

The system is supported by a comprehensive technical service, calibration with NIST-traceable standards, and comes with remote setup, training, and warranty coverage—ensuring reliability for clinical diagnostics and bioprocessing laboratories.