Eppendorf BioFlo 320 DUAL Bioprocess Control System with Autoclavable and Single-Use Vessel Support

The Eppendorf BioFlo 320 DUAL Bioprocess Control System is a versatile compact unit designed for upstream bioprocessing applications including microbial fermentation and cell culture. It supports both glass autoclavable jacketed/dish-bottom vessels and Eppendorf’s BioBLU single-use bioreactors in volumes ranging from 1 L to 50 L, making it ideal for scale-up and bioprocess development workflows in biotechnology and molecular diagnostics.

• Compatibility & Vessel Options: Compatible with multiple glass autoclavable vessels (1–10 L) and BioBLU single-use vessels (0.38 L to 50 L) suitable for microbial and cell culture processes.
• Gas Flow Control: Equipped with Thermal Mass Flow Controllers (TMFC) allowing precise regulation of up to four gases (Air, N2, O2, CO2), supporting sparge (0.002–20 SLPM) and overlay gas flows critical for controlled aeration and metabolic regulation.
• Process Monitoring & Control: Accepts PT100 temperature sensors, pH, dissolved oxygen (DO), Redox, CO2 sensors (up to two additional sensors), and has optional optical pH input. Liquid-cooled or Peltier exhaust condensers are supported to maintain controlled culture environments.
• Agitation & Pumping: Includes four integrated Watson-Marlow peristaltic pumps with variable speed control (5–100 rpm) for automated feeding and media delivery, essential in bioprocess optimization and fed-batch operations.
• Interface & Connectivity: Features a responsive ELO touchscreen interface for real-time monitoring and flexible control, with digital inputs for vessel drives and external vessel scale integration enabling accurate biomass or medium volume measurement.
• Temperature Control: Supports heated blanket accessories for BioBLU vessels and utilizes cooling loops or external chillers to precisely regulate bioreactor temperature within defined ranges (ambient +5°C up to 80°C depending on vessel).
• Applications: Widely adopted in biotechnology fields including biopharmaceutical production, gene editing process optimization, synthetic biology research, and molecular cloning workflows. Supports scale-up/scaling down studies, microbial growth optimization, and cell culture bioprocessing.
• Customization & Accessories: Offers customization options such as different vessel volumes, impeller types (pitched blade, marine, Rushton), additional pump integration, second gas regulator kits, vessel baffles, foam and level sensors, and various exhaust condensers.
• Specifications:
• Dimensions: 40.6 × 40.6 × 66.0 cm
• Weight: 32 kg
• Power: 100-120/208-240 VAC
• Warranty: 120 days USA, 60 days international warranty
• Included Components: Two BioFlo 320 controllers, ELO touchscreen monitor with arm mount, bioreactor agitation motors, 4-gas regulator valve set, pH and DO sensors with cables, temperature sensors, and bench scales. Glass vessels sold separately.

This system is essential for precise bioprocess control in live-cell imaging studies, synthetic biology research pipelines, and clinical diagnostics requiring robust bioprocess parameter monitoring and control. Its modular design enables seamless transition from lab scale to pilot biomanufacturing, making it a critical tool in biopharmaceutical production pipelines.

Keywords incorporated: bioprocess control system, bioreactor controller, single-use bioreactors, autoclavable vessels, thermal mass flow controller, peristaltic pumps, microbial fermentation, cell culture bioprocessing, biopharmaceutical production, synthetic biology research, molecular diagnostics, upstream bioprocessing, fed-batch operation, biotechnology equipment.