Eppendorf BioFlo 320 Bioprocess Bioreactor Control System with 1 SLPM Gas Flow Control

The Eppendorf BioFlo 320 Bioprocess Bioreactor Control System is engineered for versatile upstream bioprocessing workflows essential in biotechnology, molecular diagnostics, and biopharmaceutical production pipelines. This compact, modular device supports both autoclavable glass vessels (1–10 L) and BioBLU single-use bioreactors (working volumes from 65 mL to 40 L), making it critical for microbial fermentation and cell culture applications.

Key technical specifications and features include:

• Gas flow control via Thermal Mass Flow Controllers (TMFCs) with standard flow range of 0.002–1.0 SLPM and optional 0.04–20 SLPM, accommodating four gases (Air, N₂, O₂, CO₂), supporting accurate gas mixing and sparge gas delivery.
• Compatibility with various vessel types:
• Glass/Autoclavable jacketed vessels (1, 3, 5, 10 L) optimized for controlled temperature (up to 80°C max depending on vessel type).
• BioBLU single-use vessels tailored for cell culture (BioBLU c series) and microbial culture (BioBLU f series) workflows.
• Integration of digital sensor inputs supporting PT100 temperature probes, pH, dissolved oxygen (DO), redox, and CO₂ sensors, with up to two extra digital sensors configurable, and optional optical pH sensing.
• Four installed Watson-Marlow peristaltic pumps with variable speed controls (5–100 rpm) for precise feeding and media addition, extendable by up to two external peristaltic pumps.
• Supports vessel agitation drives — direct or magnetic — including options for different impeller types: pitched blade, marine, and Rushton turbines, supporting scale-up and optimization of microbial and cell culture bioprocesses.
• Controller dimensions: 40.6 cm × 40.6 cm × 66.0 cm; weight: 32 kg, with 100–120/208–240 VAC power supply, suitable for lab and pilot-scale environments.
• Touchscreen interface provides intuitive, real-time monitoring and control of critical process parameters: temperature, pH, DO, gas flow rates, agitation speed, and foam levels.
• Designed for integration in biotechnology research workflows including bioprocess development, biomanufacturing scale-up, synthetic biology experiments, and molecular cloning optimization.

The system is accompanied by a 4-gas regulator kit, temperature and pH sensors, DO sensor, and offers remote setup and operation training, along with warranty coverage (120 days continental US, 60 days international). Accessories such as heated blankets (included free for BioBLU vessel use), jacketed vessels, external chillers, exhaust condensers, vessel scales, and various sensor options extend functionality, enabling customization for specific bioprocessing needs.

Widely adopted in molecular biology, biomedical engineering, and synthetic biology applications, the BioFlo 320 is essential for managing upstream bioprocess parameters with precision, enabling controlled microbial growth optimization, cell culture fermentation, and process scale-up/down studies.

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This bioreactor control system is fully tested for performance, including sensor calibration, pump function, heating control, and gas delivery verification, providing researchers and industrial bioprocess teams with a reliable platform essential for bioprocess optimization and consistent experimental reproducibility in biotechnology, molecular diagnostics, and biopharmaceutical production environments.