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Eppendorf MasterCycler X40 Gradient Thermal Cycler with 96-Well Capacity

UsedEppendorfPCR equipmentUnited States

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Thermoblock
Aluminum
Temperature control range of the block
4 – 99 °C
Temperature Uniformity
≤±0.4°C at 95°C
Temperature control mode
Fast, Standard, Safe
Heating technology
Three Peltier elements
All 19 specifications
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Specifications 19

Brands
Eppendorf
New/Used
Used
Thermoblock
Aluminum
Temperature control range of the block
4 – 99 °C
Temperature Uniformity
≤±0.4°C at 95°C
Temperature control mode
Fast, Standard, Safe
Heating technology
Three Peltier elements
Sample capacity
96 wellplate or 0.2mL PCR tubes
Gradient range
1 – 30 °C
Gradient temperature range
30 – 99 °C
Lid temperature range
37 – 110 °C
Gradient Block
over 12 Columns
Heating rate
3.3 °C/s 1
Cooling rate
1.5°C/s 1
Interfaces
Ethernet, USB
Total weight
15 lbs
Power supply
115/130V, 50/60Hz
Max. power consumption
500 W
Dimensions
10x15x9″

Product description

Fast, precise PCR thermal cycler with 12-column gradient, 96-well block, ±0.2°C uniformity, touchscreen control.

The Eppendorf MasterCycler X40 is a high-performance gradient thermal cycler designed for advanced molecular diagnostics and biotechnology workflows. Featuring an aluminum thermoblock accommodating a standard 96-well plate or 0.2 mL PCR tubes, it supports simultaneous testing of 12 different denaturation and annealing temperature gradients across its 12-column block. This gradient thermal cycler provides tight temperature homogeneity (±0.2°C) and precise control accuracy (±0.15°C), ensuring reproducible amplification critical for applications in gene editing, molecular cloning, and synthetic biology.

Key technical specifications include:

  • Temperature control range: 4–99 °C with fast, standard, and safe control modes
  • Gradient temperature range: 30–99 °C across 12 columns with programmable gradients from 1 to 30 °C
  • Heating technology: Three Peltier elements for rapid temperature ramping
  • Heating rate: 3.3 °C/s; Cooling rate: 1.5 °C/s
  • Lid temperature: Adjustable from 37–110 °C to prevent condensation
  • User interface: Intuitive 7″ touchscreen for easy programming and gradient setup
  • Connectivity: Ethernet and USB ports for data transfer and GLP-compliant audit documentation
  • Weight and dimensions: 15 lbs; 10 x 15 x 9 inches footprint
  • Power: 115/130V, 50/60 Hz with max 500 W consumption

This gradient PCR thermal cycler integrates a user management system for secure operation and is widely used in clinical diagnostics, bioprocessing, and biopharmaceutical production pipelines requiring stringent thermal control and reproducibility. Its constant 20 °C warm-up phase reduces thermal stress on samples, optimizing reaction conditions crucial in live-cell imaging and molecular biology research.

The Eppendorf MasterCycler X40 is essential equipment for research professionals seeking reliable performance in PCR optimization, making it a valuable asset in biotechnology labs focused on gene expression analysis, synthetic biology, and biomanufacturing applications.

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Frequently asked questions

How long does laboratory equipment typically last?

With a service contract, ten to fifteen years is normal for most benchtop instruments. Detectors and pumps are the parts that wear first.

Can I get service for older equipment?

Often, yes. Verified suppliers frequently work with service partners converting the instrument. If applicable, we can list servicing options alongside the offer.

Where can I buy parts?

Ask a scientist through the request button. Parts are often available even when the instrument itself is discontinued.

What is the typical lead time for refurbished equipment?

Two to six weeks, depending on refurbishment scope and whether calibration is included.

Does laboratory equipment ship internationally?

We ship to 38 countries. Crating and customs handling are quoted with the instrument.

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Last updated 7 November 2026