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Eppendorf Mastercycler Thermal Cycler X50S with Fast Silver Block for 96-Well Plates and Tubes

UsedEppendorfPCR equipmentUnited States

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Sample Capacity
96-well plate or 0.1/0.2 mL tubes (X50S and X50i)
Temperature control mode
Fast, Intermediate, Standard, Safe
Temperature Range
4-99 °C temp
Accuracy
±0.15 °C
Block homogeneity 20 – 72 °C
≤±0.2 °C
All 17 specifications
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Specifications 17

Brands
Eppendorf
New/Used
Used
Sample Capacity
96-well plate or 0.1/0.2 mL tubes (X50S and X50i)
Temperature control mode
Fast, Intermediate, Standard, Safe
Temperature Range
4-99 °C temp
Accuracy
±0.15 °C
Block homogeneity 20 – 72 °C
≤±0.2 °C
Block homogeneity 95 °C
≤±0.3 °C
Heating Rate
10 °C/s (X50S and X50i)
Cooling rate
5 °C/s (X50S and X50i) 2.3 °C/s
Gradient range
1 – 30 °C
Gradient temperature control range
30 – 99 °C
2D-Gradient block
over 12 columns/over 8 rows (X50S and X50i)
Lid temperature range
37 – 110 °C
Dimensions
11x22x13
Weight
25 lbs
Power
100-240v, 50/60 Hz, 850W

Product description

Compact thermal cycler with fast silver block, 96-well capacity, precise temperature control (4–99 °C), ideal for PCR and molecular diagnostics.

The Eppendorf Mastercycler Thermal Cycler X50S is a robust and compact laboratory thermal cycler designed for precise polymerase chain reaction (PCR) applications, molecular diagnostics, and biotechnological workflows. Featuring a fast silver thermal block compatible with 96-well plates or 0.1/0.2 mL tubes, it delivers rapid heating (10 °C/s) and controlled cooling rates (5 °C/s). The instrument supports temperature control modes including fast, intermediate, standard, and safe, with an operating range from 4 °C to 99 °C and accuracy within ±0.15 °C.

Key features include:

  • 2D-gradient temperature control over 12 columns and 8 rows, enabling optimized protocol development for gene editing, bioprocessing, and synthetic biology research
  • Heated lid temperature adjustable between 37 °C and 110 °C to prevent condensation and ensure sample integrity
  • Network connectivity allowing standalone use or control of up to 50 units via specialized PC software, supporting high-throughput workflows in molecular diagnostics and biomanufacturing
  • Compact dimensions (11x22x13 inches) and a weight of 25 lbs suitable for benchtop placement in clinical and research laboratories

This thermal cycler is widely adopted in molecular cloning workflows and biomedical engineering applications, ensuring consistent temperature homogeneity (≤±0.3 °C) critical for reliable PCR amplification. The Mastercycler X50S supports biotechnology fields requiring stringent temperature protocols, including synthetic biology, gene editing, and qPCR assay development.

Included accessories encompass a 96-well block, user manual, and 120V power cord, backed by a 30-day warranty for laboratory assurance. The Eppendorf Mastercycler X50S combines precision temperature control, user-friendly touchscreen interface, and flexible connectivity, making it essential equipment in modern biotechnology and molecular biology research environments.

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Frequently asked questions

How long does laboratory equipment typically last?

With a service contract, ten to fifteen years is normal for most benchtop instruments. Detectors and pumps are the parts that wear first.

Can I get service for older equipment?

Often, yes. Verified suppliers frequently work with service partners converting the instrument. If applicable, we can list servicing options alongside the offer.

Where can I buy parts?

Ask a scientist through the request button. Parts are often available even when the instrument itself is discontinued.

What is the typical lead time for refurbished equipment?

Two to six weeks, depending on refurbishment scope and whether calibration is included.

Does laboratory equipment ship internationally?

We ship to 38 countries. Crating and customs handling are quoted with the instrument.

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Last updated 23 October 2026