Eppendorf Mastercycler nexus gradient PCR Thermal Cycler MC nexus gradient

The Eppendorf Mastercycler nexus gradient MC nexus gradient is a used 96-well gradient PCR thermal cycler designed for routine PCR, molecular biology, biotechnology, gene editing, synthetic biology, and molecular diagnostics workflows.

Technical specifications

• Sample capacity: 96 × 0.2 mL PCR tubes
• Compatible consumables: 96-well PCR plates, 0.2 mL PCR tubes, 0.2 mL PCR tube strips, and 0.5 mL PCR tubes
• Additional tube capacity: 8 × 12 or up to 71 × 0.5 mL PCR tubes
• Thermal block: Aluminum
• Temperature control range: 4–99 °C
• Gradient range: 1–20 °C
• Gradient temperature range: 30–99 °C
• Block uniformity: ≤ ±0.3 °C at 20–72 °C
• Control accuracy: ±0.2 °C
• Heating rate: 3 °C/s
• Cooling rate: 2 °C/s
• Noise level: Less than 40 dB(A)
• Interfaces: CAN_in/CAN_out, Ethernet, and USB
• Power supply: 100 V, 50–60 Hz
• Dimensions: 25 × 41.2 × 32.1 cm
• Weight: 10.9 kg
• Included accessory: One gray power cord

The flexible lid accommodates different vessel heights, while the aluminum block supports multiple standard PCR vessel formats without requiring an additional block. The system can be integrated with up to two eco cyclers, and an optional nexus X2 eco configuration is described for expanded workflows.

This Eppendorf gradient PCR system is suitable for thermal cycling, assay optimization, molecular cloning workflows, nucleic acid amplification, and research applications in biotechnology and molecular biology. The listed unit is used and sold as-is; actual performance may differ from new-equipment specifications.