Eppendorf Mastercycler Nexus 96-Well Gradient PCR Thermal Cycler with Peltier Heating

The Eppendorf Mastercycler Nexus is a high-precision PCR thermal cycler designed for consistent, reliable amplification in busy molecular biology and biotechnology laboratories. It supports a 96-well format compatible with 0.2 mL tubes, strips, and standard plates, enhancing flexibility in sample throughput essential for gene editing, synthetic biology, and molecular diagnostics workflows.

Key technical features include:

• Peltier-based Triple Circuit Heating Technology ensuring rapid and uniform temperature cycling
• Temperature range of 4–99 °C with ±0.2 °C accuracy, critical for sensitive bioprocessing protocols
• Maximum heating and cooling rates of 3 °C/s and 2 °C/s, respectively, enabling shortened cycle times
• Gradient capability (Model 6331) offering a 1–20 °C spread across the block for annealing temperature optimization, ideal for assay development and protocol fine-tuning
• Temperature uniformity of ≤ ±0.3 °C between 20–72 °C and ≤ ±0.4 °C at 95 °C, ensuring reproducible results in molecular cloning and gene amplification
• Flexlid system automatically adjusts lid pressure and height to different consumables, minimizing evaporation risk during extended runs
• Quiet operation at 40 dBA, suitable for shared lab environments
• Compact dimensions (16 x 10 x 13 inches) and a weight of 35 lbs promoting benchtop use in clinical and biomedical engineering labs

The thermal cycler’s versatility and robust control make it widely adopted in synthetic biology research, live-cell PCR applications, and biopharmaceutical production pipelines, where strict temperature precision and assay reproducibility are paramount. Its gradient function facilitates rapid protocol optimization—critical in molecular diagnostics and bioprocessing industries requiring precise thermal profile development.

Fully evaluated and calibrated by technicians before shipment, the Mastercycler Nexus ensures dependable performance to support routine amplification as well as complex protocol development. This equipment is suitable for researchers and professionals engaged in neuroscience, cell imaging, and various biotechnology fields that depend on reliable PCR technology.