Bio-Rad T100 Benchtop Thermal Cycler with Gradient PCR Capability

The Bio-Rad T100 Thermal Cycler is a compact, benchtop PCR system engineered for precise amplification and temperature-gradient optimization. It features a 96-well block compatible with standard-height 0.2 mL tubes, strips, or plates, supporting sample volumes from 1 to 100 µL, ideal for molecular cloning workflows and routine PCR amplification in biotechnology and molecular diagnostics.

Key technical specifications include:

• Gradient range: 30°C to 100°C with ±0.5°C programmed accuracy
• Temperature uniformity: ±0.5°C well-to-well within 30 seconds of target temperature, ensuring reliable reaction conditions critical in gene editing and synthetic biology research
• Ramp rates: Average of 2.5°C/sec and maximum up to 4°C/sec for efficient thermal cycling
• Heated lid: Minimizes condensation for consistent reaction volumes and improved reproducibility in bioprocessing applications
• Memory: Stores up to 500 typical PCR protocols with unlimited capacity via USB flash drive, facilitating protocol management for clinical diagnostics and biomanufacturing pipelines
• User interface: Full-color touchscreen simplifies programming, editing, and method retrieval, enhancing workflow efficiency in live-cell imaging and fluorescence analysis workflows

Dimensioned at 10" x 18" x 9" and weighing 20 lbs, the T100 conserves valuable laboratory bench space while delivering the robust performance expected in advanced thermal cycling. Its gradient capability supports temperature-gradient testing, a critical feature for assay optimization in biotechnology research fields like molecular cloning and synthetic biology.

This system’s reliable temperature control and flexible programming make it essential equipment in biomedical engineering, molecular biology laboratories, and clinical research settings, supporting a wide range of PCR applications foundational to molecular diagnostics and biopharmaceutical production pipelines.