Advanced Instruments Solentim Cell Metric VIPS Single Cell Seeder with Cell Metric CLD High Contrast Imager for Clonality Verification

The Advanced Instruments Solentim Cell Metric VIPS + Cell Metric CLD High Contrast Imager Monoclonality System is an integrated platform designed for single-cell seeding, monoclonality verification, and longitudinal colony growth monitoring essential in biotechnology and molecular diagnostics. It streamlines workflows in biopharmaceutical production, gene editing, and synthetic biology research by combining gentle cell handling with precise imaging technologies.

Key technical features include:

• Single Cell Seeding: Low-pressure nanoliter droplet dispensing (<1 psi) minimizes shear stress on fragile cell lines such as iPSCs, CHO, and HEK293 cells, promoting high viability and reliable plating.
• In-Well Z-Stack Imaging: 20 focal planes per well, enabling detailed 3D cell position confirmation and high-resolution imaging even at well edges, critical for accurate clonality assurance.
• Two-Step Clonality Assurance: Immediate verification of single cells post-seeding via integrated droplet imaging, followed by daily high-contrast whole-well imaging for outgrowth monitoring.
• Automated Plate Handling: Temperature-controlled stacker accommodates up to 10 plates for high-throughput workflows in cell line development and monitoring, reducing hands-on operation time.
• Imager Specifications: 10x objective magnification, 650 nm laser wavelength at max 850 µW average power, enabling high-contrast visualization of plated clones. Imaging time for clonality verification is approximately 4-5 minutes per plate.
• Software Suite: Advanced versions of Cell Metric VIPS and CLD software with GPU-accelerated image processing support functions including instrument control, clonality reports, fluorescence imaging, barcode reading, and proliferation monitoring.
• Applications: Ideal for generating clonal cell lines with regulatory-compliant documented monoclonality, improving efficiency over limiting dilution methods, and tracking stable colony growth crucial in molecular cloning workflows and biomanufacturing pipelines.

The integrated VIPS and Cell Metric CLD system is widely adopted in synthetic biology research, molecular diagnostics, and bioprocessing due to its accuracy in confirming single-cell origin and optimizing clonal expansion. With automated seeding and imaging, it significantly enhances throughput and data reliability in cell line development, live-cell imaging assays, and biomedical engineering applications.

This unit includes a computer with pre-installed software versions (VIPS 1.0.19.11056 and CLD 3.6.13.7536), power and communication cables, and a 10-plate capacity cassette loader geared for 96-well plate formats from diverse manufacturers (BD Falcon, Corning, Eppendorf, Greiner, Nunc, TPP). It underwent rigorous functionality testing including power, imaging system performance, plate handling precision, and temperature stability verification.

Overall, this pre-owned system offers a reliable, fully serviced solution for laboratories focused on clonal cell line generation with comprehensive monoclonality documentation critical to regulatory submissions and downstream applications in biopharmaceutical production and molecular biology research.