Cytiva Pall iCELLis Nano Single-Use Fixed-Bed Bioreactor System 3-Fold Configuration

The Cytiva Pall iCELLis Nano Single-Use Fixed-Bed Bioreactor System (3-Fold) is engineered for small- to mid-scale adherent cell culture bioprocessing. This system integrates three independent iCELLis Nano units, each featuring a fixed-bed surface area adjustable from 0.5 to 4 m², with bed diameters of 11 cm and selectable bed heights (2, 4, or 10 cm). Its magnetic drive impeller delivers gentle agitation within a speed range of 100 to 1500 rpm, optimized for maintaining shear-sensitive cell cultures.

Key control functionality is centered on the mPath Control Tower and Pall Link software (version 1.5.1), enabling precise real-time monitoring and regulation of critical bioprocess parameters including:

• pH control (electrochemical sensors, 0–14 range)
• Dissolved oxygen (DO) control (electrochemical sensors, 0–150% air saturation)
• Temperature control via Peltier elements (20–40°C) and reusable PT100 sensors
• Biomass sensing capability via enhanced process analytic technology probes (sensor sold separately)

The system accommodates six pre-calibrated thermal mass flow controllers (TMFCs) enabling flexible gas mixing setups with air, O₂, CO₂, and N₂ for primary and secondary gas mixtures. Gas flow rates for each gas type can be finely adjusted from 0 to 1000 mL/min, facilitating accurate control of cellular respiratory environments essential for viral vaccine production, gene therapy vector manufacturing (AAV, lentivirus), monoclonal antibody biomanufacturing, and cell-based research workflows.

Additional components provided include three PALL mPath Control Towers, three docking stations, three filter heaters, and sensor cabling for pH and DO measurements. Note that disposable bioreactor vessels, tubing manifolds, and biomass sensors are not included but are integral consumables for operation.

This 3-fold system is tailored for:

• Bioprocessing scale-up and optimization in biotechnology and clinical diagnostics
• Adherent cell culture expansion and seed train development preceding large-scale production
• Applications in molecular diagnostics, synthetic biology research, and biopharmaceutical pipelines

Compact dimensions and modular design allow efficient laboratory footprint utilization, with controller dimensions of 23 × 60 × 45 cm and docking stations at 34 × 36 × 29 cm.

The system is widely adopted in biotechnology sectors requiring controlled cell density monitoring, precise gas environment programming, and scalable single-use bioreactor solutions, critical in molecular cloning workflows and biomanufacturing of viral vectors and therapeutic proteins.

Primary keywords included: "fixed-bed bioreactor system," "single-use bioreactor," "bioprocessing," "pH and DO sensor," "thermal mass flow controller," "adherent cell culture bioreactor," and "viral vaccine production."

Long-tail biotechnology key phrases naturally embedded: "critical in biopharmaceutical production pipelines," "widely adopted in synthetic biology research," "essential for molecular cloning workflows," "viral gene therapy vector manufacturing," "optimized for monoclonal antibody production," and "small-scale flexible bioprocess runs."

This pre-owned system undergoes comprehensive testing to ensure full operational functionality, backed by warranty and technical support options, making it a reliable platform for advanced biotechnology research and production environments.