Roche LightCycler 480 II 96-Well Real-Time PCR System with Integrated Thermal Cycler and Detection

The Roche LightCycler 480 II is a 96-well real-time PCR system designed for rapid and precise nucleic acid amplification and detection. Utilizing an advanced Peltier-based silver block thermal cycler, it delivers exceptional temperature homogeneity and reproducibility across wells and cycles, critical for reliable molecular diagnostics and gene expression analysis.

Key technical features include:

• Reaction volumes from 10 µL to 100 µL per well suitable for diverse assay formats
• Temperature control range between 37°C and 95°C with the ability to perform high-resolution melting (HRM) analysis starting at 20°C
• Integrated optical detection system enabling uniform signal acquisition independent of sample position for mono-color and multiplex assays
• Compatibility with a comprehensive range of probe chemistries including SYBR Green I, ResoLight dye, hydrolysis probes, HybProbe, and SimpleProbe, facilitating flexible assay design
• Pre-installed LightCycler 480 Software featuring an intuitive interface for programming, real-time data capture, and extensive automated or manual analysis of genetic variation, mutation scanning, and quantitative PCR data

The system is essential for biotechnology workflows such as gene expression profiling, molecular cloning validation, synthetic biology research, and molecular diagnostics. It is widely adopted in biopharmaceutical production pipelines and molecular diagnostics laboratories requiring high-throughput, accurate, and reproducible real-time PCR results.

This LightCycler 480 II setup is supplied with an HP Engage Flex Pro computer and peripherals, ensuring full integration for data handling and analysis critical to genomics and bioprocessing research.