BioRad CFX96 Real-Time PCR System with Gradient and Multiplex Capabilities

The BioRad CFX96 Real-Time PCR System is a high-throughput quantitative PCR thermal cycler designed for precise nucleic acid amplification and detection in biotechnology and molecular diagnostics applications. Featuring a 96-well sample capacity compatible with 1–50 µL reaction volumes, this instrument employs six independently controlled Peltier thermal electric modules for rapid temperature ramping (up to 5°C/s) and uniform thermal regulation (±0.4°C well-to-well at 90°C).

Key technical features include:

• Multiplex detection of up to 5 fluorescent targets using solid-state optics with 6 filtered LEDs and 6 photodiodes covering excitation/emission wavelengths from 450–730 nm, enabling reliable multiplex assays and singleplex FRET analyses.
• Gradient thermal cycling capabilities with an operational range of 30–100°C and programmable gradient span from 1–24°C for simultaneous optimization of annealing temperatures across up to 8 different settings.
• High sensitivity sufficient to detect single-copy targets in human genomic DNA, with a 10-order dynamic fluorescence detection range.
• Fast scanning times, achieving data acquisition in as little as 3 seconds for FAM/SYBR Green chemistries and 12 seconds for all channels, enhancing throughput in bioprocessing and synthetic biology workflows.
• Intuitive onboard software paired with a bright LCD interface facilitates protocol optimization through automatic identification of ideal cycling parameters based on polymerase and primer characteristics.
• Modular design supporting tool-free exchange of thermal blocks and flexible operation as a standalone instrument or networked configuration controlling up to 4 units via PC-based CFX software.

This real-time PCR system is widely adopted in gene expression analysis, molecular cloning, synthetic biology research, and molecular diagnostics. Its precise temperature control and multiplex fluorescence detection make it critical for biopharmaceutical production pipelines, gene editing validation, and advanced biomedical engineering studies.

Dimensions are 14" x 19" x 15" with a weight of 50 lbs, connected via USB 2.0 interface for streamlined data management. The heated lid reaches up to 105°C to prevent condensation, ensuring reproducibility during thermal cycling.

Overall, the BioRad CFX96 combines robust thermal cycling performance with versatile fluorescence detection, making it essential for molecular biology researchers seeking reliable quantitative PCR data in high-throughput biotechnology environments.