Bio-Rad QX600 AutoDG Droplet Digital PCR System with Automated Droplet Generator, PX1 Plate Sealer, and C1000 Touch Thermal Cycler

The Bio-Rad QX600 AutoDG Droplet Digital PCR System is an advanced microfluidic platform engineered for precise nucleic acid quantification and robust molecular diagnostics. It features the AutoDG Automated Droplet Generator that partitions samples into approximately 20,000 highly uniform microdroplets per 20 µL reaction, enhancing assay precision critical in biotechnology applications such as gene expression analysis, mutation detection, and copy number variation (CNV) assessment.

Key Components and Specifications:

• AutoDG Automated Droplet Generator:
• Sample input volume: 20 µL
• Capacity: 96 samples per run
• Generates ~20,000 droplets/sample for high sensitivity and reproducibility
• QX600 Droplet Reader:
• Detects up to 12 targets multiplexed in a single well
• 6 fluorescent detection channels (FAM/EvaGreen, HEX/VIC, Cy5, Cy5.5, ROX, ATTO 590)
• Utilizes LED illumination and avalanche photodiode detectors for high signal fidelity
• Absolute quantification sensitivity ≥0.1%
• Capacity: 96 samples per run
• C1000 Touch Thermal Cycler with 96-Deep Well Module:
• Compatible with 96 x 0.2 mL tubes, 48 x 0.5 mL tubes, or one 96-well plate
• Temperature range: 0–100 °C with ±0.2 °C accuracy
• Maximum ramp rate: 2.5 °C/sec
• Memory capacity for over 1,000 PCR protocols
• Step-based graphical and automatic programming modes
• PX1 PCR Plate Sealer:
• Heat-up time under 3 minutes
• Sealing temperature range: 100–190 °C
• Adjustable sealing duration: 0.5–10 seconds

System Integration and Workflow Advantages:

• Seamless integration of droplet generation, thermocycling, and automated plate sealing reduces operator-induced variability, enhancing reproducibility in sensitive molecular diagnostics and bioprocessing workflows.
• High-throughput capability supports 96-well plate runs, ideal for clinical diagnostics, NGS assay validation, and drug discovery assays requiring multiplex detection.
• Automated microfluidic droplet partitioning and advanced fluorescence detection improve precision in molecular cloning, synthetic biology research, and biopharmaceutical production pipelines.

Applications:

• Mutation and variant detection in clinical and research settings
• Absolute quantification in gene expression and CNV studies
• Validation workflows for next-generation sequencing (NGS) assays
• Multiplexed target detection for environmental biotechnology, including wastewater monitoring and AAV genome integrity
• Essential for molecular diagnostics in biomedical engineering and biomanufacturing processes

This system comes fully serviced, tested for fluidics, thermal cycling performance, droplet uniformity, and software communication. It includes a computer preloaded with Bio-Rad software, power and communication cables, and remote setup/training options. The package is backed by a continental US 120-day warranty and international 60-day warranty, offering post-sale technical support and quality assurance highly valued in academic and industrial biotechnology laboratories.

---

This unit is optimized for professionals engaged in biotechnology, molecular biology, clinical diagnostics, and related fields who require a reliable, automated droplet digital PCR solution widely adopted in contemporary research and industry.