Bio-Rad CFX Opus 96 Real-Time PCR System with Gradient Thermal Cycling and Multiplex Fluorescence Detection

The Bio-Rad CFX Opus 96 Real-Time PCR System is engineered for advanced molecular diagnostics, biotechnology research, and quantitative gene expression analysis. This 96-well qPCR instrument is equipped with a Peltier-based thermal cycler capable of precise temperature control between 4°C and 100°C, featuring a gradient function programmable from 1–24°C to optimize assay conditions across samples.

• Thermal performance: Maximum ramp rate of 5°C/sec (average 3.3°C/sec), heated lid adjustable from 30–110°C ensures uniform amplification and minimizes condensation.
• Optical system: Solid-state optics with six filtered LEDs (excitation wavelengths 450–684 nm) and six filtered photodiodes (emission wavelengths 515–730 nm) enable sensitive, multiplexed fluorescence detection across diverse chemistries, including SYBR Green, TaqMan, and hydrolysis probes.
• Detection sensitivity: Capable of detecting a single copy of target sequences within human genomic DNA, suitable for high-sensitivity molecular cloning, gene editing validation, and molecular diagnostics workflows.
• Connectivity & control: Supports Ethernet and Wi-Fi connections for remote operation; includes a tiltable touchscreen interface and compatibility with Windows 10-based control software, facilitating seamless integration into networked laboratory environments.
• Sample capacity & handling: Standard 96-well block supporting 1–50 µL reaction volumes (recommended 10–50 µL), ideal for high-throughput qPCR assays in genomics, synthetic biology, and bioprocessing research.
• Dimensions & power: Compact footprint (13"x26"x14"), weighing 48 lbs, with universal power input (100–240V, 50/60Hz), suitable for diverse laboratory settings.

This instrument is critical in biotechnology and biomedical engineering applications, widely used in gene expression profiling, synthetic biology research, and biopharmaceutical development where precision thermal cycling and multiplex fluorescence analysis are essential. Its robust dynamic range spanning 10 orders of magnitude supports quantitative analyses across a broad spectrum of nucleic acid concentrations, making it indispensable for molecular cloning workflows, molecular diagnostics, and bioprocessing pipelines.