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Applied Biosystems ABI ViiA 7 Real-Time PCR System with Multiplex Fluorescence Detection and OptiFlex Optics

UsedApplied BiosystemsPCR equipmentUnited States

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Detection Method:
Primer-Probe Detection, SYBR
Reaction Volume Range:
10-100 µl
Run Time:
About 35 min. (varies depending on reagents and other factors)
Dynamic Range:
Up to 9 logs of linear dynamic range
Optics:
6 emission filters (500-720 nm) 6 excitation filters (450-670 nm) OptiFlex System (enhanced fluorescence detection)
All 16 specifications
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Specifications 16

Brands
Applied Biosystems
New/Used
Used
Detection Method:
Primer-Probe Detection, SYBR
Reaction Volume Range:
10-100 µl
Run Time:
About 35 min. (varies depending on reagents and other factors)
Dynamic Range:
Up to 9 logs of linear dynamic range
Optics:
6 emission filters (500-720 nm) 6 excitation filters (450-670 nm) OptiFlex System (enhanced fluorescence detection)
Program Features:
Remote monitoring of up to 15 instruments
Reaction Speed:
Standard
Resolution:
Detects as little as 1.5-fold changes in target quantities (in singleplex reactions)
Sensitivity:
Down to 1 copy
Temperature Range (Metric):
4-100°C
Temperature Uniformity:
±0.5°C
Dimensions
21x26x25″
Power
100–240 V, 50/60 Hz
Weight:
140 lbs

Product description

Compact real-time PCR with multiplex detection, 6-channel OptiFlex optics, compatible with 384/96-well plates and microfluidic arrays.

The Applied Biosystems ABI ViiA 7 Real-Time PCR System is a precision instrument designed for quantitative PCR (qPCR) workflows in molecular diagnostics, biotechnology, and bioprocessing research. Featuring a compact footprint, this system supports multiple interchangeable block formats including 384-well plates, 96-well plates, and TaqMan Micro Fluidic Cards, enabling high-throughput and flexible sample processing.

Key technical features include:

  • OptiFlex system with 6 decoupled excitation (450-670 nm) and emission filters (500-720 nm) to support robust multiplex fluorescence detection and enhance sensitivity.
  • Reaction volume range from 10 to 100 µL with temperature control from 4°C to 100°C and ±0.5°C uniformity, providing accurate thermal cycling conditions critical for molecular cloning, gene expression analysis, and synthetic biology workflows.
  • Capability to detect as little as 1 copy of target DNA and discern 1.5-fold changes in singleplex assays, essential for precise molecular quantification and diagnostics.
  • Remote monitoring of up to 15 instruments and user-friendly touchscreen with one-button protocols to streamline biomanufacturing and lab automation integration.
  • Compatibility with TaqMan Micro Fluidic Cards enables molecular diagnostics platforms and high-throughput qPCR applications popular in clinical research and biotechnology.

This system is widely adopted in molecular biology, biomedical engineering, and biopharmaceutical production pipelines for gene editing validation, synthetic biology experiments, and high-throughput fluorescence-based quantitative assays. Integrated software supports multiple analysis methods for reliable data recovery from over 100 runs, making it a versatile tool for advanced live-cell imaging studies, gene expression quantification, and fluorescence analysis.

Included with the system are a Windows 11 workstation running QuantStudio RT software, 384-well block with heated lid, communication cables, user manual, and power cord. The ABI ViiA 7 is compliant with 21 CFR Part 11 when equipped, further supporting regulated environments in molecular diagnostics and biomedical research.

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Frequently asked questions

How long does laboratory equipment typically last?

With a service contract, ten to fifteen years is normal for most benchtop instruments. Detectors and pumps are the parts that wear first.

Can I get service for older equipment?

Often, yes. Verified suppliers frequently work with service partners converting the instrument. If applicable, we can list servicing options alongside the offer.

Where can I buy parts?

Ask a scientist through the request button. Parts are often available even when the instrument itself is discontinued.

What is the typical lead time for refurbished equipment?

Two to six weeks, depending on refurbishment scope and whether calibration is included.

Does laboratory equipment ship internationally?

We ship to 38 countries. Crating and customs handling are quoted with the instrument.

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Last updated 5 November 2026