Applied Biosystems 7900HT Fast Real-Time PCR System with 96/384-Well High-Throughput Capability

The Applied Biosystems 7900HT Fast Real-Time PCR System is a robust instrument designed for *high-throughput molecular diagnostics*, gene expression analysis, and SNP genotyping applications central to biotechnology and clinical research. This system supports both 96- and 384-well thermal cycling blocks, enabling flexible throughput for bioprocessing and molecular cloning workflows.

Key features include:

• Fast real-time PCR capability with reduced run times (~33 minutes for 96-well in Fast Mode), optimizing throughput in synthetic biology and biomanufacturing projects.
• Continuous wavelength fluorescence detection (500–660 nm) utilizing calibrated dyes such as SYBR Green I, ROX, FAM, VIC, and others, essential for multiplex PCR assays.
• Sensitive detection down to 10 DNA copies, supporting applications in molecular diagnostics and pathogen quantitation.
• Thermal cycling precision with temperature accuracy ±0.25°C and uniformity ±0.5°C, ensuring reproducibility critical for quantitative PCR in gene editing validation.
• Hands-free sample plate loading automation and optional Enterprise software enable streamlined data management and workflow automation required in biopharmaceutical production pipelines.
• Compatible with a reaction volume range of 10–30 µL and equipped with optics including a 488 nm Argon-ion laser and CCD spectrograph for robust fluorescence detection.

The system’s dynamic range extends up to 9 logs, making it ideal for high-sensitivity applications such as molecular cloning, synthetic biology research, and gene expression profiling in clinical diagnostics and biotechnology. Its interoperability with multiplexing and high-throughput formats renders it indispensable for labs requiring reliable performance in bioprocessing and advanced molecular research environments.