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Used, in good condition
Peltier-based 96-well real-time PCR system with CCD optics, fast thermal cycling, and multiplex dye detection.
The Applied Biosystems 7500 Fast Real Time PCR System is a high-performance thermal cycler optimized for quantitative molecular diagnostics and biotechnological applications such as gene expression analysis, synthetic biology, and molecular cloning workflows. Featuring a Peltier-based 96-well block, it enables rapid thermal cycling with a peak heating rate of 5.5°C/sec and sample ramp rates of 3.5°C/sec in fast mode and 1.6°C/sec in standard mode, supporting reaction volumes from 10 to 30 µL.
The instrument incorporates a CCD camera paired with a halogen lamp for fluorescence detection, utilizing 5 excitation and 5 emission filters to accurately detect multiple fluorophores. It comes calibrated for key dyes including FAM/SYBR Green, VIC/JOE, NED/TAMRA/Cy3, ROX/Texas Red, and Cy5, supporting multiplex assays essential for advanced molecular diagnostics and bioprocessing pipelines. The system optionally uses ROX or other calibrated passive reference dyes to normalize signal variability.
Designed to accommodate 96-well low-profile PCR strip plates or 8-tube strips, the system ensures precise temperature control with a range from 4°C to 99.9°C, an accuracy of ±0.5°C, and uniformity within ±1°C. Its compact footprint (340 x 450 x 490 mm) and weight (34 kg) make it suitable for core laboratories engaged in clinical diagnostics, biotechnology research, and biomanufacturing processes.
This real-time PCR instrument is widely adopted in synthetic biology research and critical in biopharmaceutical production pipelines, offering reliable performance for quantitative PCR assays in neuroscience, cell imaging, and molecular diagnostics requiring fast, accurate, and multiplex-capable thermal cycling technology.
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With a service contract, ten to fifteen years is normal for most benchtop instruments. Detectors and pumps are the parts that wear first.
Often, yes. Verified suppliers frequently work with service partners converting the instrument. If applicable, we can list servicing options alongside the offer.
Ask a scientist through the request button. Parts are often available even when the instrument itself is discontinued.
Two to six weeks, depending on refurbishment scope and whether calibration is included.
We ship to 38 countries. Crating and customs handling are quoted with the instrument.
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Last updated 5 November 2026
The middle half of the 393 with a price runs from €201 to €3,070.
1 other listing of this model is available.
