Hyclone 25mM Tris, 75mM NaCl, 300mM Sucrose Buffer Solution, pH 7.4

The Hyclone 25mM Tris, 75mM NaCl, 300mM Sucrose, pH 7.4 Solution is a precisely formulated laboratory buffer commonly used in cell biology, molecular diagnostics, and biotechnology research. This solution contains:

• 25 mM Tris as a buffering agent, stabilizing physiological pH conditions
• 75 mM Sodium Chloride (NaCl) to maintain osmotic balance
• 300 mM Sucrose to provide osmotic support ideal for cell and tissue sample preservation
• Adjusted to pH 7.4, mimicking physiological conditions critical for live-cell imaging and biochemical assays

This buffer solution is widely utilized in workflows involving cell lysis, subcellular fractionation, and protein isolation, especially in neuroscience and synthetic biology fields. Its composition supports bioprocessing protocols and is essential for maintaining cellular integrity during molecular cloning and gene editing procedures. The stable buffering capacity and osmolarity make it suitable for applications in biopharmaceutical production pipelines requiring reliable cell culture reagents.

Ideal for research environments demanding high reproducibility, the Hyclone solution is a standard component in protocols involving fluorescence analysis, live-cell imaging, and biomedical engineering projects.