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Bio-Rad CFX96 Real-Time PCR Detection System with Multiplex 5-Channel Fluorescence and Deep Well 96-Well Thermal Cycler

UsedBio-RadPCR equipmentUnited States

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Description
The Bio-Rad CFX96 Real-Time PCR Detection System is a 96-well thermal cycler optimized for real-time fluorescence detection across multiple channels. It provides accurate temperature control and uniformity with a Peltier-based heating/cooling system, coupled with high-resolution photomultiplier tube detectors. The instrument supports multiplex PCR with 5-color multiplexing capability and features the CFX Manager software for assay setup, data acquisition, and analysis.
Technical Data
Model: CFX96 Software Version: CFX Manager v3.1 Lasers: Count: 5 Wavelengths: 450 nm (blue), 494 nm (green), 530 nm (yellow), 630 nm (red), 690 nm (far-red) Manufacturing Year: 2018
Software Version
CFX Manager v3.1
Lasers
Count: 5 Wavelengths: 450 nm (blue), 494 nm (green), 530 nm (yellow), 630 nm (red), 690 nm (far-red)
Count
5
All 12 specifications
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Specifications 12

Brands
Bio-Rad
New/Used
Used
Description
The Bio-Rad CFX96 Real-Time PCR Detection System is a 96-well thermal cycler optimized for real-time fluorescence detection across multiple channels. It provides accurate temperature control and uniformity with a Peltier-based heating/cooling system, coupled with high-resolution photomultiplier tube detectors. The instrument supports multiplex PCR with 5-color multiplexing capability and features the CFX Manager software for assay setup, data acquisition, and analysis.
Technical Data
Model: CFX96 Software Version: CFX Manager v3.1 Lasers: Count: 5 Wavelengths: 450 nm (blue), 494 nm (green), 530 nm (yellow), 630 nm (red), 690 nm (far-red) Manufacturing Year: 2018
Software Version
CFX Manager v3.1
Lasers
Count: 5 Wavelengths: 450 nm (blue), 494 nm (green), 530 nm (yellow), 630 nm (red), 690 nm (far-red)
Count
5
Wavelengths
450 nm (blue), 494 nm (green), 530 nm (yellow), 630 nm (red), 690 nm (far-red)
Manufacturing Year
2018
Accessories
Optical calibration plate, Standard 96-well plate holder, AC power cord, User manual
Dimensions
20.2 × 18.8 × 15.7 in
Weight
51 lbs

Product description

High-sensitivity Bio-Rad CFX96 real-time PCR system with rapid thermal cycling and 5-channel multiplex fluorescence detection.

The Bio-Rad CFX96 Real-Time PCR Detection System is a precision-engineered thermal cycler optimized for *quantitative and qualitative PCR analysis* in biotechnology and clinical diagnostics. Featuring a 96-well deep well block, this instrument enables rapid thermal cycling with accurate and uniform temperature control via a Peltier-based heating and cooling system.

  • Multiplexing Capability: Supports up to 5 fluorescent channels, including blue (450 nm), green (494 nm), yellow (530 nm), red (630 nm), and far-red (690 nm) wavelengths, enabling complex multiplex PCR assays essential for gene expression profiling, pathogen detection, and mutation analysis.
  • Detection System: High-resolution photomultiplier tube detectors provide sensitive fluorescence measurement, critical for applications such as *molecular diagnostics, synthetic biology*, and *bioprocessing* where precise quantification is required.
  • Software: Equipped with CFX Manager v3.1, the intuitive software facilitates assay setup, data acquisition, and comprehensive analysis workflows widely adopted in *molecular biology research* and *biopharmaceutical production pipelines*.
  • Design and Usability: Compact footprint (20.2 × 18.8 × 15.7 inches) and robust mechanical components ensure reliability and durability in high-throughput laboratory environments.
  • Accessories Included: Optical calibration plate, standard 96-well plate holder, AC power cord, and user manual streamline deployment in research and clinical laboratory settings.

The Bio-Rad CFX96 system is *critical for advanced biotechnological workflows* such as gene editing validation, live-cell gene expression analysis, and molecular cloning workflows, making it a valuable asset in *neuroscience*, *cell imaging*, and *clinical diagnostics laboratories*. Its multi-channel fluorescence detection and rapid thermal cycling capabilities make it a versatile choice for researchers requiring high throughput and multiplex analysis in molecular diagnostics and synthetic biology research.

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Frequently asked questions

How long does laboratory equipment typically last?

With a service contract, ten to fifteen years is normal for most benchtop instruments. Detectors and pumps are the parts that wear first.

Can I get service for older equipment?

Yes. Every verified supplier states which service partners cover the instrument, and we list that alongside the offer.

Where can I buy parts?

Ask a scientist through the request button. Parts are often available even when the instrument itself is discontinued.

What is the typical lead time for refurbished equipment?

Two to six weeks, depending on refurbishment scope and whether calibration is included.

Does laboratory equipment ship internationally?

We ship to 38 countries. Crating and customs handling are quoted with the instrument.

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Last updated 19 October 2026