Thermo Scientific Gibco CTS Xenon Electroporation Instrument A50301 25mL Closed Transfection System

The Thermo Scientific Gibco CTS Xenon Electroporation Instrument (Model A50301) is a high-performance, closed electroporation system designed for efficient cell transfection in research and commercial bioprocessing workflows. Engineered to support electroporation volumes ranging from 1 mL up to 25 mL, it enables scalable delivery of DNA, RNA, proteins, and other molecules into hard-to-transfect cells while maintaining high cell viability and recovery.

Key features include:

• Closed, single-use electroporation chamber ensuring GMP compliance and minimized contamination risk, essential for cell therapy manufacturing and biomanufacturing pipelines
• Support for recommended cell concentrations of 20 x 10^6 to 100 x 10^6 cells/mL, accommodating a broad range of biotechnology applications such as gene editing, molecular diagnostics, and synthetic biology research
• Adjustable pulse parameters: pulse voltage of 500–2,500 V, pulse width of 1–30 ms, pulse interval of 500–1,000 ms, and up to 10 pulses per run, enabling optimization for diverse cell types
• Run times between 7–22 minutes for large volumes (5–25 mL), facilitating throughput suitable for biopharmaceutical production pipelines
• Integration compatibility with Invitrogen Neon NxT Electroporation System and Thermo automation software for a closed, controlled transfection workflow compliant with 21 CFR part 11 regulations
• Robust build with dimensions of 109.5 x 67.4 x 53.9 cm and weight of approximately 83.8 kg, designed for lab environments focusing on biotechnology and biomedical engineering

This instrument is widely adopted in molecular biology and cell therapy development settings, making it critical for workflows involving live-cell electroporation, gene editing technologies like CRISPR, and scalable cell transfection in biotech and clinical diagnostics laboratories. Note: The system is preowned, in good working condition, and sold without the computer interface.

This electroporation instrument is essential for researchers and professionals aiming to accelerate translational research and scale up protocols from bench-scale experiments to commercial cell therapy manufacturing processes.