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ProteinSimple Wes Automated Capillary Western Blot System for Protein Separation and Immunodetection

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Proteins size range
2-440 kDa
Minimum sample size
3uL per capillary
Capillary
5 cm, 100 µm, 400 nL
Maximum Number of Samples
25 (75 samples per day)
Sizing CV
<10%
All 15 specifications
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Specifications 15

Brands
ProteinSimple
New/Used
Used
Proteins size range
2-440 kDa
Minimum sample size
3uL per capillary
Capillary
5 cm, 100 µm, 400 nL
Maximum Number of Samples
25 (75 samples per day)
Sizing CV
<10%
Intra-assay CV
<15%
Resolution (± percent difference in MW)
± 15-20% for MW <20 kDA ± 10% for MW >20 kDa
Dynamic Range
2-3 logs (Total Protein Specification)3-4 logs (Immunoassay Specification)
Sensitivity
ng(Total Protein Specification)Low pg (Immunoassay Specification)
Process Time
3 hours
Dimensions
14x21x13”
Weight
50lbs
Power
100-240vac, 50/80Hz, 510W

Product description

Automated capillary Western blot system enabling protein separation and immunodetection with high sensitivity and minimal sample volume.

The ProteinSimple Wes Western Blot System is an automated, capillary-based platform designed for precise protein separation and immunodetection, widely adopted in molecular diagnostics and biotechnology research. It supports high-throughput analysis, processing up to 24 samples per run with a total assay time of approximately 3 hours, optimizing workflows in bioprocessing and synthetic biology.

Key technical features include:

  • Capillary electrophoresis using 5 cm, 100 µm capillaries with 400 nL volume capacity
  • Protein size range capability from 2 to 440 kDa, suitable for a broad spectrum of protein targets
  • Minimal sample volume requirement of 3 µL per capillary to reduce reagent use and assay variation
  • Dynamic detection range spanning 3-4 logs for immunoassays and 2-3 logs for total protein, supporting quantitative molecular diagnostics
  • Sensitivity down to low picogram levels for immunodetection, essential for biomarker validation and biopharmaceutical research
  • Integration with Compass software for advanced assay analysis, 21 CFR Part 11 compliance, digital capillary imaging, and robust data management

This system automates all critical steps: sample loading, size-based separation, immunoprobing, washing, detection, and analysis, replacing labor-intensive traditional Western blotting. Its high reproducibility (sizing CV <10%, intra-assay CV <15%) and advanced HDR detection technology ensure reliable results critical in biomanufacturing pipelines and live-cell protein expression studies.

The ProteinSimple Wes is distinct from multiplex ELISA instruments like the ProteinSimple Ella, focusing on capillary-based Western blotting rather than immunoassay multiplexing. It is an essential laboratory instrument for researchers in cell imaging, neuroscience, and biotechnology, streamlining workflows in gene editing and molecular cloning projects with enhanced accuracy and throughput.

Why Questpair exists

Lab equipment markets are fragmented. Buyers spend weeks calling distributors, comparing quotes, and verifying supplier reliability. Sellers struggle to reach global research labs.

We built Questpair to fix this. Every supplier on the platform is verified before listing. Every quote you receive comes from a real, vetted seller. AI prepares your case in minutes, then a real specialist takes over.

We're an independent team based in The Netherlands, working with research labs and procurement teams across Europe and worldwide.

Frequently asked questions

How long does laboratory equipment typically last?

With a service contract, ten to fifteen years is normal for most benchtop instruments. Detectors and pumps are the parts that wear first.

Can I get service for older equipment?

Yes. Every verified supplier states which service partners cover the instrument, and we list that alongside the offer.

Where can I buy parts?

Ask a scientist through the request button. Parts are often available even when the instrument itself is discontinued.

What is the typical lead time for refurbished equipment?

Two to six weeks, depending on refurbishment scope and whether calibration is included.

Does laboratory equipment ship internationally?

We ship to 38 countries. Crating and customs handling are quoted with the instrument.

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Last updated 19 October 2026