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Procurable · sourced on request

MilliporeSigma Immobilon-P PVDF Membrane

UsedMilliporeSigmaImmunohistochemistryNetherlands
Procurable

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Pore Size
0.45 µm
Filter Dimensions
270 mm x 3750 mm
Insulin Adsorption Capacity
160 µg/cm²
BSA Adsorption Capacity
215 µg/cm²
Goat IgG Adsorption Capacity
294 µg/cm²
Availability
Procurable
Lead time
Confirmed with your quote
All 13 specifications
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Specifications 13

Brands
MilliporeSigma
New/Used
Used
Pore Size
0.45 µm
Filter Dimensions
270 mm x 3750 mm
Insulin Adsorption Capacity
160 µg/cm²
BSA Adsorption Capacity
215 µg/cm²
Goat IgG Adsorption Capacity
294 µg/cm²
Primary Binding Mechanisms
Electrostatic, Hydrophobic
Wettability
Hydrophobic
Detection Methods
Chemiluminescent, Chromogenic, Radioactive
Compatible Stains
Coomassie brilliant blue, Amido black, India ink, Ponceau-S red, Colloidal gold, CPTS, Toluidine blue, Transillumination, Sypro® ruby
Availability
Procurable
Lead time
Confirmed with your quote

Product description

Hydrophobic PVDF membrane with 0.45 µm pores for high-capacity protein transfer and multiple detection methods.

Immobilon-P PVDF Membrane is a hydrophobic polyvinylidene fluoride (PVDF) transfer membrane designed for western blotting and dot blot applications. This membrane offers high protein-binding capacity, ensuring efficient protein transfer and detection.

Key features include:

  • 0.45 µm pore size: Ideal for proteins greater than 20 kDa, facilitating effective transfer and retention.
  • Hydrophobic nature: Enhances protein binding through electrostatic and hydrophobic interactions.
  • Mechanical durability: Resists cracking, curling, and fracturing, allowing easy handling and cutting.
  • Low background: Provides clear results with various detection methods, including chemiluminescent, fluorescent, radioactive, chromogenic, and chemifluorescent techniques.
  • Reprobing capability: Supports multiple probing cycles without loss of sensitivity.

This membrane is widely used in:

  • Molecular biology: For protein analysis and characterization.
  • Biotechnology: In applications such as protein sequencing and immunoblotting.
  • Clinical diagnostics: For detecting disease markers through protein assays.

Commonly employed in:

  • University laboratories: For academic research and teaching.
  • Biotechnology companies: In product development and quality control.
  • Clinical research facilities: For diagnostic assay development.

Since its introduction, the Immobilon-P PVDF Membrane has remained a standard in protein blotting applications, continuing to be relevant and widely used in laboratories worldwide.

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Frequently asked questions

How long does laboratory equipment typically last?

With a service contract, ten to fifteen years is normal for most benchtop instruments. Detectors and pumps are the parts that wear first.

Can I get service for older equipment?

Often, yes. Verified suppliers frequently work with service partners converting the instrument. If applicable, we can list servicing options alongside the offer.

Where can I buy parts?

Ask a scientist through the request button. Parts are often available even when the instrument itself is discontinued.

What is the typical lead time for refurbished equipment?

Two to six weeks, depending on refurbishment scope and whether calibration is included.

Does laboratory equipment ship internationally?

We ship to 38 countries. Crating and customs handling are quoted with the instrument.

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Last updated 26 October 2026