Sartorius Biostat B Twin Benchtop Bioreactor with 1 L Jacketed Autoclavable Glass Vessels for Cell Culture

The Sartorius Biostat B Twin Bioreactor is a benchtop stirred-tank system designed for advanced cell culture applications, featuring two autoclavable 1-liter double-wall jacketed glass vessels optimized for precise temperature regulation via a recirculating cooling water system. Each vessel includes a direct-drive 200 W motor capable of agitation speeds from 20 to 2000 rpm employing a low-shear 3-blade segment impeller, critical for maintaining controlled hydrodynamic conditions in mammalian cell culture processes such as CHO and HEK cell line cultivation.

Key technical features include:

• Integrated gas management system: 4-gas mixing (Air, O2, N2, CO2) with ring sparger aeration and headspace overlay, utilizing thermal mass flow controllers (TMFCs) for *±1% full scale precision*—essential for optimizing dissolved oxygen (DO) and pH levels in bioprocess development.

• Advanced process control: Twin control tower supporting dual vessels with multiple probe ports for pH, dissolved oxygen (DO), and temperature sensors (Pt100 with ±0.1 °C accuracy), enabling real-time monitoring and regulation critical in biopharmaceutical production pipelines and synthetic biology research.

• Built-in peristaltic pumps (Watson Marlow 114) for acid/base addition and medium feed support fine-tuned environmental control and dosing strategies, vital for seed train optimization and media/feed strategy testing.

• Temperature control module: Open thermostat system with 600 W heating capacity and recirculation pump, capable of maintaining vessel temperatures from 8 °C above the cooling water inlet temperature up to 80 °C, suitable for mammalian and microbial culture temperature ranges.

• Robust digital interfacing: RS-232/M12 ports facilitate integration with balances, external feed pumps, exhaust cooling, and overlay aeration controls, supporting complex bioprocess workflows and automation.

This system is particularly widely adopted in molecular diagnostics and bioprocess development labs, enabling small-scale parallel bioreactor experimentation necessary for upstream biomanufacturing, gene editing research, and synthetic biology workflows. Its compact 35 × 82.2 × 43 cm footprint and weight of 55 kg make it suitable for laboratory benches, supporting fast process development cycles with reliable instrumentation.

The Biostat B Twin is supplied with operational testing verification, technical consultation, remote setup, and user training, ensuring scientific reproducibility and operational efficiency. It supports precise control of culture parameters, critical in live-cell imaging preparations, fluorescence-based assays, and neurobiological cell line cultivation.

Applications include:

• Mammalian cell culture process development and optimization
• Seed train scale-up preparation
• Parallel bioprocess screening and media strategy evaluations
• Biopharmaceutical production and quality control

This pre-owned unit is fully tested to industry calibration standards with NIST-traceable flowmeters and sensor calibrations, backed by a warranty. Optional accessories and customized vessel sizes are available to tailor the system for specific experimental or production needs in biotechnology research and industrial bioprocessing.

Keywords integrated: benchtop bioreactor, jacketed glass vessel, cell culture bioreactor, stirred-tank bioreactor, biopharmaceutical production, bioprocess development, mass flow controller, dissolved oxygen sensor, mammalian cell culture, biotechnology research, molecular diagnostics, biomanufacturing, gene editing workflows, synthetic biology applications.