Sartorius Biostat B Benchtop Dual-Vessel Cell Culture Bioreactor System with Integrated pH and DO Control

The Sartorius Biostat B Benchtop Dual Fermentation Cell Culture Bioreactor System is a compact, versatile bioprocessing platform designed for parallel cultivation of cell cultures with robust control over critical bioprocess parameters. This system is widely adopted in biotechnology research and molecular diagnostics, supporting scale-down modeling and media optimization experiments essential for biopharmaceutical production pipelines.

Key technical features include:

• Dual 2-Liter autoclavable univessel reactors supporting working volumes from 0.6 to 2 liters, allowing simultaneous parallel bioprocesses for enhanced throughput and experiment reproducibility.
• Motor-driven agitation with a 200 W direct drive unit capable of stirring speeds between 20 and 2000 rpm. The system uses two 3-blade segment impellers per vessel, ensuring gentle mixing ideal for shear-sensitive mammalian cell cultures.
• Integrated pH control and foam management pumps, enabling precise bioprocessing control for long-term culture applications in bioprocess development and synthetic biology workflows.
• Advanced gas control module with four solenoid valves for regulated supply of Air, O₂, N₂, and CO₂ gases, coupled with five rotameters for manual flow rate adjustments (±5% full scale accuracy). Flow rates cover typical bioreactor gas supply ranges (e.g. O₂, N₂, CO₂ up to 300 ccm). This gas regulation is critical for maintaining optimal dissolved oxygen levels and pH in cell culture bioreactors.
• Temperature control via 185 W external heating blankets combined with an optional cooling loop, facilitating precise temperature management from 8°C above cooling water temperature up to 80°C, supporting various bioprocess conditions.
• Multiple integrated sensors including the Hamilton EasyFerm Plus PHI VP 225 pH sensors, VisiFerm ECS225 dissolved oxygen sensors, and temperature probes, ensuring reliable, real-time monitoring critical for process control in biomedical engineering and biomanufacturing environments.
• Peristaltic pumps (two Watson Marlow 114 fixed-speed for feed and addition, plus pumps dedicated to base addition and antifoam control) provide precise reagent delivery for maintaining culture environment stability during bioprocess operation.

This system excels in cell culture process development, media optimization, and scale-down bioprocess modeling, making it essential equipment for molecular cloning workflows, gene editing research, and bioprocess control optimization in biopharmaceutical production pipelines. The modular design supports customization with options such as alternative impellers and chillers, adapting to specific bioprocess requirements.

The Sartorius Biostat B system features an intuitive touchscreen interface with digital control, facilitating ease of use in busy research laboratories. Its dual bioreactor configuration is ideal for comparative studies and reproducible experimental design. The system undergoes comprehensive functionality testing, including calibration of gas flow, pH and dissolved oxygen sensors, and agitation motor verification, ensuring reliable performance on delivery.

The benchtop footprint combined with a full warranty and after-sales technical support makes this bioreactor system a popular choice among molecular biology researchers, clinical diagnostics labs, and bioprocess engineers involved in synthetic biology and bioprocess development. It is widely used in live-cell imaging and fluorescence analysis studies that require tightly controlled culture conditions.

Keywords integrated: Benchtop bioreactor system, cell culture bioreactor, dual fermentation bioreactor, dissolved oxygen control, pH regulation, biotechnology research equipment, bioprocess control, synthetic biology research, molecular diagnostics instrument, biomanufacturing process development.