Eppendorf DASbox Mini Bioreactor System Unit 2 for Small-Scale Bioprocess Development

The Eppendorf DASbox Mini Bioreactor System Unit 2 is a modular, parallel cultivation platform designed to support small-scale bioprocess development in biotechnology and cell culture research. It features four independent bioreactors optimized for *precise control* of critical parameters including pH, dissolved oxygen (DO), and temperature, enabling reproducible experimental conditions essential for bioprocess optimization and scale-down studies.

Key technical specifications include:

• Parallel bioreactor units: 4 vessels operating simultaneously
• Working volumes: 60–250 mL for glass vessels; 100–250 mL for single-use vessels (vessels not included)
• Agitation: Overhead drive with speed ranges of 20–2,500 rpm (glass) and up to 500 rpm (single-use impellers)
• Temperature control: Liquid-free Peltier system enabling heating and cooling with ranges of 10–60 °C (glass) and 10–45 °C (single-use)
• Gas flow control: Thermal mass flow controllers (TMFC) handle gas mixtures of Air, N₂, O₂, and CO₂ with flow rates from 0.04 to 5 sL/h, supporting overlay or sparger gassing strategies
• pH control: Automated via CO₂/base addition with flexible system configurations
• DO control: Cascade control using O₂ concentration and gas flow rate adjustments for fine-tuned oxygenation
• Additional features: Peltier-based exhaust condensation preventing liquid waste, two feeding lines per vessel for media or reagent addition

This system is widely adopted in *biotechnology* and *molecular diagnostics* for applications such as cell culture optimization, microbial cultivation, media screening, and early-stage bioprocess development. It is critical for workflows in *synthetic biology*, *biopharmaceutical production pipelines*, and *bioprocess scale-up modeling* due to precise parameter control and scalability.

The DASbox Mini Bioreactor System supports both traditional glass and single-use vessels, facilitating diverse bioprocessing strategies including biomanufacturing and gene editing research. Its compact footprint and parallel design provide an efficient platform for process development requiring multiple conditions tested simultaneously.

Included in the Unit 2 package:

• DASbox MP8-PHPO with PH4PO4LS option
• DASbox Base controller
• Four Peltier exhaust condensers
• Four magnetic overhead drives
• Four temperature probes
• Power and communication cables

*Note*: Reaction vessels, consumables, and software are sold separately. Configuration and full operational setup may require manufacturer support. This system is sold as-is without warranty or software.

Keywords: Mini bioreactor system, parallel bioreactor, cell culture bioreactor, microbial bioreactor, small-scale bioprocessing, biotechnology development, bioprocess optimization, synthetic biology research, molecular diagnostics, biopharmaceutical scale-down, Peltier temperature control, TMFC gas mixing, dissolved oxygen monitoring, pH control in bioprocessing.