Eppendorf BioFlo 320 DUAL Bioprocess Control System for Autoclavable and Single-Use Bioreactors

The Eppendorf BioFlo 320 DUAL is a versatile bioprocess control system designed for laboratory-scale fermentation and cell culture applications. It supports autoclavable glass vessels (1 to 10 L) and BioBLU single-use vessels, providing flexible options for microbial production and mammalian cell culture bioprocessing — essential for molecular diagnostics, synthetic biology, and biopharmaceutical development workflows.

Key technical features include:

• Modular Controller: Integrated touchscreen interface with advanced control of agitation, temperature, pH, dissolved oxygen (DO), and gas mixing parameters for precise bioprocess monitoring and control.
• Gas Management: Equipped with Thermal Mass Flow Controllers (TMFCs) facilitating independent control of up to four gases (Air, N₂, O₂, CO₂) with sparge and overlay flow options spanning 0.002 to 20 SLPM, supporting aerobic and anaerobic process optimization.
• Compatibility: Supports both glass autoclavable jacketed or dish-bottom vessels and a wide range of BioBLU single-use vessels (0.3 L to 50 L working volumes), enabling scale-up/down experiments and disposable bioprocessing strategies favored in biomanufacturing.
• Sensor Integration: Accepts PT100 temperature probes, pH, DO, Redox, and CO₂ sensors, including an optional optical pH sensor, enabling comprehensive process analytical technology (PAT) approaches in biotechnology research.
• Agitation and Pumps: Dual bioreactor drive with direct or magnetic vessel drives; four built-in Watson-Marlow peristaltic pumps with adjustable speed for nutrient feeding and media exchange critical in continuous culture and fermentation process control. Optional external pumps available.
• Liquid Handling: Supports external chilled water supply and cooling loops with operating temperature ranges up to 80°C, allowing precise temperature regulation required for mammalian cell culture and microbial bioprocesses.
• Advanced Features: Includes vessel scale interface for real-time mass monitoring, level and foam sensors, heated blankets for single-use vessels, and Peltier exhaust condensers.

This control system is widely adopted in biotechnology laboratories, bioprocess development, and biopharmaceutical production pipelines, where real-time process control and scalable bioreactor platforms are critical. It is essential for upstream bioprocess optimization, live-cell bioreactor monitoring, and cell culture scale-up studies.

The BioFlo 320 DUAL comes fully tested and serviced with a 120-day US warranty, remote setup training, and technical support, ensuring reliability and rapid deployment in research or production environments. Its compact bench-scale footprint and configurable options make it ideal for laboratory-scale bioprocess control in biotechnology, molecular cloning workflows, and synthetic biology research.

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