Eppendorf BioFlo 120 Bioprocess Controller for BioBLU Single-Use and Glass Vessels

The Eppendorf BioFlo 120 Bioprocess Controller is a compact bioprocess control system for microbial fermentation and mammalian, insect, bacterial, yeast, or fungal cell culture. It supports Eppendorf BioBLU single-use vessels and glass/autoclavable vessels from 250 mL to 40 L, making it suitable for cell line development, protein expression, vaccine production, media optimization, and small-scale biomanufacturing.

Control and Process Management

• Built-in touchscreen interface
• Control of pH, dissolved oxygen (DO), temperature, agitation, and gas mixing
• Four-gas mixing capability for air, N2, O2, and CO2
• One thermal mass flow controller with a gas-out sparge flow range of 0.04–20 SLPM
• Additional thermal mass flow controller ranges may include 0.002–1 SLPM or 0.01–5 SLPM
• Three installed peristaltic pumps with Watson-Marlow 114DV heads
• Pump speed: 30 rpm, with 0–100% duty cycle
• Direct or magnetic vessel drive connection
• PT100 temperature probe compatibility
• Two digital sensor inputs for pH, DO, redox, or CO2 sensors
• One level sensor input
• Heater blanket control
• Compatibility with liquid or Peltier exhaust condensers
• Advanced models may provide three analog input/output connections

Vessel Compatibility

The BioFlo 120 can be configured for:

• Eppendorf BioBLU single-use vessels: BioBLU 1c, 1f, 3f, 10f, 3c, 5c, 5p, 14c, and 50c, including µ or M variants where applicable
• Glass/autoclavable vessels: 1.0 L, 2.0 L, 5.0 L, and 10 L
• Cell culture vessels with pitched-blade or packed-bed impellers
• Fermentation vessels with Rushton-type impellers

Reported BioBLU working-volume ranges include 320 mL–1.25 L for BioBLU 1c, 250 mL–1.25 L for BioBLU 1f, 1.25–3.75 L for BioBLU 3c and 3f, 3.75 L for BioBLU 5p, 3.5–10.5 L for BioBLU 14c, and 18–40 L for BioBLU 50c.

Included Configuration

• Eppendorf BioFlo 120 bioprocess control station
• Vessel-matched drive, quantity 1
• Four-gas regulator with valves, quantity 1
• pH sensor cable, quantity 1
• pH sensor, quantity 1
• Dissolved oxygen sensor, quantity 1
• Temperature sensor, quantity 1
• Heated blanket for BioBLU vessel applications, with size selected according to vessel configuration

Technical Specifications

• Power requirement: 100–120/208–240 VAC
• Dimensions (W × D × H): 24.7 × 55.9 × 62.9 cm (9.7 × 22 × 24.8 in)
• Net weight: 14.8 kg (32.7 lb)
• Gas supply pressure with glass/autoclavable vessels: maximum 10 psig (0.69 barg)
• Gas supply pressure with BioBLU single-use vessels: maximum 6 psig (0.44 barg)
• Liquid-cooled exhaust condenser pressure: 0.5 psig (0.035 barg)

This pre-owned bioprocess controller is tested for touchscreen operation, agitation control, peristaltic pump delivery, pH and DO sensor readings, heating control, temperature accuracy, gas output, and accessory functionality. It is suited to biotechnology workflows including microbial fermentation, cell culture, bioprocess development, molecular biology research, and small-scale biopharmaceutical production.