Eppendorf BioFlo 120 Bioprocess Control System with 5 L Glass Autoclavable Vessel

The Eppendorf BioFlo 120 Bioprocess Control System is a compact benchtop controller for microbial fermentation, cell culture, protein expression, vaccine production, media optimization, and small-scale biomanufacturing. This configuration includes a 5 L glass autoclavable vessel with a working-volume range of 2.0–5.6 L.

Control and Connectivity

• Integrated touchscreen control for pH, dissolved oxygen (DO), temperature, agitation, gas mixing, and level monitoring
• Supports direct-drive or magnetic-drive vessel connections
• Accepts PT100 temperature probes
• Two digital sensor inputs for pH, DO, redox, or CO2 sensors
• One level-sensor input
• Optional advanced model configuration with three analog input/output connections
• Supports an exhaust condenser and heater blanket

Gas Flow and Pumping

• One installed thermal mass flow controller (TMFC)
• Four-gas mixing for air, nitrogen, oxygen, and carbon dioxide
• Gas supply pressure up to 10 psig (0.69 barg) with glass or autoclavable vessels
• Gas supply pressure up to 6 psig (0.44 barg) with BioBlu single-use vessels
• Sparge flow range: 0.04–20 SLPM
• Additional available TMFC ranges: 0.002–1 SLPM and 0.01–5 SLPM
• Three installed peristaltic pumps with Watson-Marlow 114DV heads
• Pump speed: 30 rpm fixed speed, 0–100% duty cycle

Vessel Compatibility

The BioFlo 120 supports glass/autoclavable vessels with nominal volumes of 1.0 L, 2.0 L, 5.0 L, and 10 L, as well as Eppendorf BioBLU single-use vessels. The included configuration uses a 5 L glass autoclavable vessel.

Available vessel configurations may include water-jacketed or heat-blanketed designs, direct-drive or magnetic-drive agitation, Rushton, pitched-blade, marine, or spin-filter impellers, and optional baffles, sparge attachments, cooling loops, level sensors, foam sensors, redox sensors, CO2 sensors, and exhaust condensers.

Technical Specifications

• Power requirement: 100–120/208–240 VAC
• Dimensions (W × D × H): 24.7 × 55.9 × 62.9 cm
• Dimensions (W × D × H): 9.7 × 22 × 24.8 in
• Net weight: 14.8 kg (32.7 lb)
• Autoclavable vessel operating range: 8°C above coolant to 40°C, or 45°C for 10 L vessels, depending on vessel type
• Liquid-cooled exhaust condenser pressure: 0.5 psig (0.035 barg)

The BioFlo 120 is suitable for biotechnology workflows including fermentation development, cell-line development, recombinant protein expression, molecular biology research, and small-scale bioprocessing. Its integrated sensor inputs, automated gas control, peristaltic pumping, and touchscreen operation support reproducible cultivation and biomanufacturing processes.