Sartorius Biostat B-CC Twin Bioreactor Controller with 5L and 10L Single-Wall Glass Univessels for Cell Culture

The Sartorius Biostat B-CC Twin is a benchtop bioreactor controller system designed for precise cell culture process control with integrated management of pH, dissolved oxygen (DO), temperature, agitation, and automated gas flow regulation. It includes dual control modules to support simultaneous cultivation in 5L and 10L Single-Wall (SW) glass Univessels, making it widely adopted in biotechnology, molecular diagnostics, and bioprocessing workflows.

Key technical features include:

• Dual independent control units enabling parallel operation of two vessels, allowing optimization of batch, fed-batch, and perfusion bioprocesses.
• 5L and 10L SW glass cultivation vessels equipped with two pitched impellers each for homogenous mixing, critical for sensitive mammalian and insect cell culture applications.
• Agitation system: 200 W direct drive motor with agitation range from 20–1,500 rpm (5L) and 20–800 rpm (10L), providing gentle shear conditions necessary for cell viability.
• 4-gas mixing system: Includes Air, O2, N2, and CO2 with thermal mass flow controllers (±1% accuracy) and solenoid-controlled rotameters for precise sparging and overlay gas supply, replicating CO2 incubator conditions essential for live-cell cultures.
• Integrated peristaltic pumps: Two fixed-speed Watson Marlow pumps for base and feed delivery, facilitating automation in nutrient addition and pH adjustment, critical in bioprocess optimization and scale-up studies.
• Temperature control: External heating blankets (435W for 5L, 780W for 10L) combined with exhaust coolers enable a control range of 8°C above cooling water inlet temperature up to 60°C, ensuring stable environment for metabolic and growth kinetics assessments.
• Advanced sensor inputs: Multiple digital ports for Pt100 temperature sensors, optical pO2 sensors, combined pH/Redox electrodes, substrate weight sensors, and inline biomass measurement systems (BioPAT ViaMass), supporting comprehensive process monitoring and control.
• Compact footprint: Dimensions 35×82.2×43 cm, weighing 55 kg, ideal for benchtop implementation in research laboratories specializing in biopharmaceutical development, synthetic biology, and molecular biology.
• Applications: Suitable for mammalian and insect cell culture, seed train development, media and feed strategy evaluation, metabolic studies, and small-scale bioproduction.
• Utility: The system’s precise control and flexibility are critical in biomanufacturing pipelines, gene editing research, and live-cell imaging preparations, widely used by molecular biology researchers and clinical diagnostics labs.

This system is favored for its reliability and comprehensive process integration, offering researchers an effective platform for cell culture process development with meticulous control over environmental parameters and nutrient supply. Its robust gas mixing and sensor integration make it essential for biotechnological applications requiring reproducible and scalable results.

Relevant long-tail keywords naturally embedded: benchtop bioreactor controller for cell culture, single-wall glass bioreactor vessels, precise pH and DO control for mammalian cell culture, 4-gas mixing bioreactor system, peristaltic pump bioreactor for fed-batch processes, cell culture bioreactor for metabolic studies, bioprocessing in synthetic biology research, biopharmaceutical production scale-up bioreactor.

This pre-owned unit is fully tested, serviced, and includes remote setup and training support, making it ideal for university laboratories, research institutions, and biotech companies focused on advanced cell culture and molecular diagnostics.