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Spectroscopy

Spectroscopy is one of the most used tools to explore properties of matter and is an umbrella term for different techniques such as infrared spectroscopy, visible light spectroscopy, UV-light spectroscopy, X-ray spectroscopy, Raman spectroscopy and nuclear magnetic resonance. Spectroscopy instruments can be stand-alone devices, but are increasingly integrated as part of other equipment, for example in combination wit (more)h confocal microscopy.

Bruker LC-NMR-MS Interface-cover
Bruker LC-NMR-MS Interface

The LC-NMR-MS Interface is designed to sit between an HPLC system, an NMR spectroscopy probehead and (more) an MS unit. It would normally be mounted on top of the MS table immediately in front of the MS input connector. The BNMI-HP consists of 3 medium pressure (> 60Bar) syringe pumps, a 1:20 splitter, an 8-Port valve, 3 mixing chambers (T-Pieces) and associated control and interface electronics. The flow of the HPLC separations to the MS unit can be modified by pumping additional solvent from the syringe pumps. This improves the MS peak detection and shortens the time needed for the split separation to reach the MS. Separations can also be stored as a result of an MS peak detection as well as through UV absorption peak detection. The splitter splits the fluid flow from the HPLC in a 1:20 ratio. i.e. ca. 5% of the fluid is directed to the MS and the remainder to either the NMR probehead for immediate analysis or to the BPSU36 storage cassette for storage and later analysis.  The fluid directed to the MS is mixed in the mixing chamber (T-Piece) with the solvent pumped from the syringe pumps. The two left hand syringe pumps pump alternatively to guarantee a constant ’makeup’ flow. The 3rd pump at the right hand side is used to pump a calibration fluid into the flow path to allow the MS to be calibrated (or simply synchronized) at the start and end of each measurement cycle. The 8-Port valve is used to direct the fluid flow. Control of the BNMI Unit is performed using the HyStar program via an ethernet interface. Commands received from HyStar are interpreted in the BNMI Control Board. The control board then sets up the BNMI unit accordingly e.g.: Starts the pumps, changes the flow rate, turns the 8-Port valve. The splitter has a fixed mechanical function and cannot be controlled or adjusted. It simply accepts a fluid flow and splits it into 2 paths in a 1:20 ratio. However, other split ratios are available (for example 1:100 may be preferred if you have very high sample concentrations).

€1,499.00 ex VAT
Netherlands
nmr spectrometers
Shimadzu UV-1800 UV/Vis Spectrophotometer-cover
Shimadzu UV-1800 UV/Vis Spectrophotometer

The UV-1800 is an advanced high-resolution spectrophotometer utilizing a precision Czerny-Turner opt (more)ical system. Operation can be either as a stand-alone instrument or as a PC-controlled instrument with the included UV Probe software. USB memory can be connected directly to the UV-1800 for simple data transfer and printing is possible with the optional screen-copy printer or printers that support PCL control codes. Photometric Mode measures the absorbance or transmittance at a single wavelength or at multiple (up to eight) wavelengths. In multiple-wavelength measurement, calculations can be performed on the data obtained for up to four wavelengths, including the calculation of the difference between, or ratio of, the measurements obtained for two wavelengths. Spectrum Mode obtains sample spectra using wavelength scanning. Changes in the sample can be tracked using repeated scans. Data processing operations, such as the enlargement or reduction of the spectra obtained, peak detection, and area calculations, are also possible. Quantitation Mode generates a calibration curve from standard samples, and uses it to calculate the concentrations of unknown samples. Various combinations of wavelengths (1 to 3 wavelengths and derivatives) and calibration curves (K-factor and first-to-third order regression calibration curves) are possible. Kinetics Mode measures the change in absorbance as a function of time, and calculates enzymatic activity values. You can select either the kinetics measurement method or the rate measurement method. Time-Scan Mode measures the change in absorbance, transmittance, or energy as a function of time. Multi-Component Quantitation Mode separately quantitates up to eight components mixed in a single sample. Pure components or mixtures of components can be used as standard samples. Biomethod Mode is capable of analyzing DNA and proteins using the several common quantitation methods including quantitation of DNA or protein using the absorbance at 260/230 nm or 260/280 nm and protein quantitation via the Lowry method, BCA method, CBB method, Biuret method, and UV absorption method (direct measurement at 280 nm). Security and validation functionality are also preprogrammed. Security functions allow you to restrict functions according to three user levels: administrator, developer, and operator. Automatic or semiautomatic validation functions include wavelength accuracy, wavelength repeatability, resolution, stray light, photometric accuracy, photometric repeatability, baseline flatness, baseline stability, and noise level. The operating time of the lamps can be recorded and displayed so that you can anticipate replacement and avoid downtime. Stray Light: <0.02%T at 340 nm, 400 nm; <1.0%T at 198 nm Source Lamp: Deuterium; Tungsten-Halogen Beam Type: Double Bandwidth: 1.0 nm Min Wavelength: 190 nm Max Wavelength: 1100 nm Wavelength Accuracy: ±0.1 nm Wavelength Reproducibility: ±0.1 nm Min Photometric – Transmittance: 0%T Max Photometric – Transmittance: 400%T Min Photometric – Absorbance: -4 A Max Photometric – Absorbance: 4 A Photometric Drift: <0.0003 A/hr Detector: Silicon photodiode Including UV Probe software

€2,499.00 ex VAT
Netherlands
spectrophotometers uv vis & fluor
Perkin Elmer 343 Polarimeter-cover
Perkin Elmer 343 Polarimeter

Polarimetry is an important technique for the investigation and analysis of optically active substan (more)ces. Common applications include: Characterization of newly synthesized compounds Quality control of pharmaceutical drugs Precise quantitative analysis of sugars Selective detection of optically active components by HPLC Monitoring and control of enzymatic reactions Principle: Automatic precision Polarimeter with optical null principle by rotation of the analyzer; automatic gain control. Radiation: Na spectral lamp Spectral lines: Selectable from instrument keypad (Na 589 nm) Apertures: Selectable apertures for standard cells and microcells. Detector: Photomultiplier 1P28A. Polarizer and analyzer: Vis-transmitting polarizers Rotatory range: ± 85° Balancing speed: 1.3°/s at 50 Hz; 1.5°/s at 60 Hz. Accuracy: ± 0.002° for rotations ≤1º; ± 0.2% for rotations >1° Reproducibility: Better than 0.002° (for zero and rotational reading). Internal angular resolution: 1 x 10-4 º Integration times: 0.1s, 0.2s, 0.5s, 1s, 2s, 5s, 10s, 20s, 50s and 100s. Autozero setting: Electronically from instrument keypad at any desired position in the rotatory range. User interaction: Either menu-driven from integrated touch-keys (English, German, French, Spanish and Italian), or PC-controlled from POL WinLab or POL WinLab ES software. Display: Stand-alone operation: vacuum fluorescence display, two lines with 20 characters each. Format of results: Selectable from instrument keypad – angular rotation, specific rotation, molar rotation, international sugar scale, user-defined scale with factor and offset. Dimensions (WxHxD): 825 x 310 x 390 mm Weight: 40 kg

€5,999.00 ex VAT
Netherlands
polarimeter, polarimeters
Perkin Elmer Model 343 S Polarimeter-cover
Perkin Elmer Model 343 S Polarimeter

Polarimetry is an important technique for the investigation and analysis of optically active substan (more)ces. Common applications include: Characterization of newly synthesized compounds Quality control of pharmaceutical drugs Precise quantitative analysis of sugars Selective detection of optically active components by HPLC Monitoring and control of enzymatic reactions Principle: Automatic precision Polarimeter with optical null principle by rotation of the analyzer; automatic gain control. Radiation: Na spectral lamp Spectral lines: Selectable from instrument keypad (Na 589 nm) Apertures: Selectable apertures for standard cells and microcells. Detector: Photomultiplier 1P28A. Polarizer and analyzer: Vis-transmitting polarizers Rotatory range: ± 85° Balancing speed: 1.3°/s at 50 Hz; 1.5°/s at 60 Hz. Accuracy: ± 0.002° for rotations ≤1º; ± 0.2% for rotations >1° Reproducibility: Better than 0.002° (for zero and rotational reading). Internal angular resolution: 1 x 10-4 º Integration times: 0.1s, 0.2s, 0.5s, 1s, 2s, 5s, 10s, 20s, 50s and 100s. Autozero setting: Electronically from instrument keypad at any desired position in the rotatory range. User interaction: Either menu-driven from integrated touch-keys (English, German, French, Spanish and Italian), or PC-controlled from POL WinLab or POL WinLab ES software. Display: Stand-alone operation: vacuum fluorescence display, two lines with 20 characters each. Format of results: Selectable from instrument keypad – angular rotation, specific rotation, molar rotation, international sugar scale, user-defined scale with factor and offset. Dimensions (WxHxD): 825 x 310 x 390 mm Weight: 40 kg

€5,999.00 ex VAT
Netherlands
polarimeters, polarimeter
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