Flow cytometers

Lasers: Blue Solid State: 488 nm, 55 mW, Software Controlled Red Solid State Diode: 638 nm, 50 mW, S (more)oftware Controlled Violet Solid State: 405 nm, 80 mW, Software Controlled Configuration: Nominal 90 µm spatially separated beam spots Flow cell: 430 µm x 180 µm rectangular channel Collection optics: Fixed integrated optics and quartz flow cell design with a numerical aperture of > 1.2 Detector filters: Forward Scatter: 488/20 Blue Laser: 525/40, 575/30, 614/20 , 675/20, 695/30, 755LP Dyes: FITC, PE, ECD, PC5 or PEC5.5, PECy7 Red Laser: 660/20, 725/20, 755 LP Dyes: APC or Alexa Fluor 647, APC-Alexa Fluor 700, APC-Cy7, APC-Alexa Fluor 750 Violet Laser: 450/50, 550/40 Dyes: Pacific Blue, Krome Orange Forward Scatter Detector: Fourier design providing up to 3 measurements of forward angle Side Scatter Detector: High performance photodiode with electronic attenuation Fluorescence Detectors: FL1 – FL6 Fluorescent Detectors Flow rates: Continuous pressure is applied to the sample tube based on user selected flow rates: Low, Medium and High Performance: Carryover: < 0.1%, Dead volume as low as 2 µL when using Beckman Coulter 12 x 75 mm polypropylene tube and adjustable probe Acquisition modes: 32 tube Multi Carousel Loader (MCL), Single tube sampling mode, Automated work list acquisition, Manual work list mode Mixing: The MCL vortexes each tube individually before sample acquisition Barcode reading: Carousel number, tube location, and tube barcode verified to worklist Biosafety: Biohazard contained wash station thoroughly rinses sample probe Throughput: 10000 normal leukocytes is 82 tubes/hour Scatter resolution: Resolves 0.4 micron particles from background noise using Forward Scatter, Maximum detection of up to 40 microns Fluorescence sensitivity: FITC < 107 MESF, PE < 64 MESF, PC5 < 13 MESF Acquisition rate: 25000 events per second, 90% yield Cytometer dimensions (WxHxD): 950 x 610 x 730 mm Cytometer weight: 104 kg Supply cart dimensions (WxHxD): 724 x 298 x 495 mm Supply cart weight: 30 kg Software included but requires a new licence purchase.

The Agilent xCELLigence Real-Time Cell Analysis (RTCA) SP instrument uses label-free cellular impeda (more)nce to continuously monitor cell health, behavior, and function with high accuracy, sensitivity, and reproducibility. The SP model can run a 96-well electronic microplate (E-Plate 96), which is ideal for cell health characterization, immune cell-mediated killing, viral cytopathic effects, cytotoxicity, cell adhesion, cell barrier function (TEER), cell signaling (e.g., GPCR) and other related applications. xCELLigence RTCA SP model can run one 96-well plate Measure cell health, viability, or response to treatments with high sensitivity, accuracy, and reproducibility Obtain continuous data at high temporal resolution (from seconds to days), with non-invasive and label-free real-time impedance technology Easy workflow allows users to simply add cells to E-plates and begin kinetic measurements at physiological conditions, with minimal hands-on time Intuitive RTCA Software Pro allows for analysis of real-time data to generate diverse plot types and automatically calculate parameters such as % of cytolysis, IC50 or KT50 RTCA Software Pro supports FDA 21 CFR Part 11 compliance to ensure electronic data integrity

The Countess 3 Automated Cell Counter is a benchtop cell counting system equipped with an advanced m (more)achine-learning algorithm, ruggedized optics, fully-automated focus and lighting, and image analysis software for rapid assessment of primary and immortalized cell samples. Capacity: 2 samples per slide Detection Method: Brightfield Memory: On Board, USB Drive Sample Type: Immortalized cell lines & primary cells For Use With (Application): Cell culture & analysis workflows For Use With (Equipment): EVOS microscopes, Attune flow cytometers Dimensions (LxWxH): 229 x 229 x 140 mm Weight: 3.6 kg

Met One offers battery-operated portability, up to six size channels and a built-in printerall in on (more)e simple, compact instrument. The Met One 237 counts airborne particles in up to six different size ranges simultaneously. Count data is displayed on the front-panel as total number of particles (cumulative count) or the particles in each size range (differential count). The bright LED display lets you see the data easily and the printout shows cumulative and differential count data for each size range. Channel Sizes: 2 ch; 0.5 µm, 5.0 µm – 4 ch; 0.5 µm, 0.7 µm, 1.0 µm, 5.0 µm – 5 ch; 0.5 µm, 0.7 µm, 1.0 µm, 2.0 µm, 5.0 µm – 6 ch; 0.5 µm, 0.7 µm, 1.0 µm, 2.0 µm, 3.0 µm, 5.0 µm Flow Rate: 0.1 CFM (2.83 LPM) Coincidence Limit: 2 million/ft3 Light Source: Laser diode Data Storage: 500 samples, rotating buffer Sample and Hold Times: 1 second to 24 hours Count Alarms: 1 to 9,999,999, counts each channel Count Modes: Manual, auto, concentration, beep Concentration Mode: 1 second samples, 1 to 10 samples averaged, display updated every second; displays counts/ft3, liter or counts/1000L (m3) Battery Recharge Time: 16 hours (with unit off) Battery Operating Time: 4 hours with printing, 8 hours without printing Dimensions (WxhXD): 170 x 120 x 300 mm Weight: 3.1 kg

The Countess II Automated Cell Counter is a fully automated cell counter and assay platform that use (more)s state-of-the-art optics and image analysis algorithms to analyze trypan blue-stained cells in suspension. Processing time: ~15 seconds Sample concentration range: 1 x 10⁴ to 1 x 10⁷ cells/mL Particle/cell diameter range: 4 60 µm (particles), 7 – 60 µm (cells) Required sample volume: 10 µL Optics: 1 channel (brightfield) Camera: 5 Mega pixels, 2.5x Optical Magnification

The Countess II Automated Cell Counter is a fully automated cell counter and assay platform that use (more)s state-of-the-art optics and image analysis algorithms to analyze trypan blue-stained cells in suspension. Processing time: ~15 seconds Sample concentration range: 1 x 10⁴ to 1 x 10⁷ cells/mL Particle/cell diameter range: 4 60 µm (particles), 7 – 60 µm (cells) Required sample volume: 10 µL Optics: 1 channel (brightfield) Camera: 5 Mega pixels, 2.5x Optical Magnification

The HORIZON system from Halo Labs employs Backgrounded Membrane Imaging (BMI) technology to measure  (more)and characterize subvisible particles in a high-throughput, low volume format. BMI has its roots in membrane microscopy – a USP lot release method that images particles on a membrane surface. The fully automated, fluidics-free HORIZON system utilizes disposable membrane plates and automated image processing to rapidly generate reliable and accurate particle counts and sizing. It can perform particle characterization in up to 96 samples in a single run using as little as 25 µL per sample. BMI is also insensitive to solution refractive index, enabling reliable measurement of translucent protein aggregates. The HORIZON system uses sophisticated imageprocessing techniques to analyze images and acquire particle data. The key is to first take a background image of the membrane. After samples are filtered through by vacuum and the particles are captured, the same membrane is re-imaged with sample particles on the surface. The sample image is precisely aligned with the background image and then processed pixel by pixel so that the background texture is eliminated, and particles are viewed in high contrast. Backgrounding the image leads to high reproducibility and minimal interference from membrane texture and imperfections.

Partec Cyflow Space FACS stand alone system with Analytical cuvette. Equipped with: 4 measurable par (more)ameters: FCS, SSC, D3 and D4 bandpass filters (expandable to 9 parameters). Blue diode laser 20 mW 488 nm (expandable to 3 laser light sources). Sorting unit with green laser (sorting cuvette not included, but can be ordered from Sysmex). Fujitsu PC with Flowmax software for flow data analysis. Bottle rack for 1 liter bottles (for e.g. sheat fluids, buffers and waste).

The UF-100 is a fully automated urine cell analyzer using flow cytometry method combined with fluore (more)scent dye and electric resistance method to detects casts. This unit is further automated tham UA-1000/2000. All the process from mixing and aspirating of sample to output of analyzed parameters are performed automatically. Analysis includes quantitative analysis and flagging. Quantitative analysis measures: RBC, WBC, epithelial cells, casts and bacteria count (concentration) in the unit of µL or view field. Flagging marks: Path, CAST (casts containing organized structures),  SRC (small round epithelial cell), YLC (yeast liked fungi cell), X’TAL (crystal), SPERM (spermatozoa) and outputs the flagging informarion (information of their presence). Analyzed results are displayed on screen and if required printed by card type printer of color printer, and they are transmitted to a host computer. Dimensions (WxHxD): 600 x 705 x 606 mm Weight: 68 kg

Imaging performance Cell size: 5 – 300 microns (Cellometer CHT4-PD300 Slides are required for cells  (more)> 80 microns in diameter) Concentration range: 105 – 107 cells/ml Bright field imaging, fluorescent imaging and pattern-recognition software to quickly and accurately decluster, identify and count individual cells Fluorescent optics Excitation/Emission: 470nm/535nm Example fluorophores: Acridine Orange (+DNA) Calcein AM CFDA SYTO 9 SYTO 13 Excitation/Emission: 540nm/605nm Example fluorophores: Propidium iodide Ethidium bromide Dimensions (WxDxH): 280 x 300 x 330 mm Weight: 11.8 kg

Address the full range of current and future multi-parameter cellular analysis research needs with t (more)he NovoCyte flow  ​cytometer. ACEA brings researchers high performance flow cytometry at a low investment cost with the NovoCyte platform. Lasers: Blue 488 nm, Red 640 nm Detectors: 530, 585, 675 nm Laser Configuration: Spatially separated beams with 10 x 80 μm elliptical spots Optical Alignment Procedure: Fixed, no operator alignment required Flow Cell: 170 x 290 μm rectangular quartz flow cell FSC/SSC Resolution: FSC < 0.5 μm, SSC < 0.2 μm Cell Size: 0.2 – 50 μm Fluorescence Threshold Sensitivity: FITC < 75 MESF, PE < 50 MESF Fluorescence Resolution: < 3% CV for CEN Filters: User Exchangeable Sample Acquisition Rate: 35,000 events/second Volumetric Absolute Count Precision: CV < 5% Sample Flow Rate: 5 – 120 μL/min Sheath Flow Rate: 6.5 mL/min Sample Aspiration Volume: 10 – 100 μL Fluid Container Capacity: 3 L sheath, 3 L waste, 500 mL cleaning, 500 mL decontamination Carryover: < 0.1% Fluidics Maintenance: Automated startup, cleaning, decontamination and shutdown Manual Sample Loading: 12 x 75 mm tube Dimension (WxDxH): 600 x 450 x 390 mm Weight: 39 k