Halo Labs Horizon Particle Analyzer

The HORIZON system from Halo Labs integrates Backgrounded Membrane Imaging (BMI) technology to accurately measure and characterize subvisible particles in a high-throughput, low volume format. Originating from membrane microscopy, a USP lot release method, BMI images particles on a membrane surface. This fully automated, fluidics-free HORIZON system employs disposable membrane plates and automated image processing for quick, reliable particle counts and sizing. Capable of characterizing particles in up to 96 samples per run using only 25 µL per sample, BMI is unaffected by solution refractive index, ensuring accurate measurement of translucent protein aggregates. Sophisticated image processing techniques are used to analyze and acquire particle data, beginning with a background image of the membrane. After filtering samples by vacuum and capturing particles, the membrane is re-imaged with sample particles on its surface. The sample image is meticulously aligned with the background and processed pixel by pixel to remove background texture, achieving high contrast for the particles. This imaging approach yields high reproducibility and minimizes interference from membrane texture or imperfections.