Flow cytometers

Hersteller: Beckman Coulter Modell: CytoFLEX V0-B3-R2 Beschreibung: Das Gerät verfügt über eine Kapa (more)zität von 15 Parametern, darunter 13 für die Fluoreszenzdetektion. Das vollständig aktivierte Gerät verfügt über fünf Kanäle des Lasers mit 405 nm (Violett), fünf Kanäle des Lasers mit 488 nm (Blau) und drei Kanäle des Lasers mit 638 nm (Rot). Laser Spezifikationen: Violet 405 nm 80 mW Blue 488 nm 50 mW Red 638 nm 50 mW Durchflusszelle: Fest integrierte Optik und Quarzdurchflusszelle mit >1,3 numerischer Apertur. Abmessungen der Durchflusszelle: 430 μm x 180 μm Innendurchmesser Vorwärtsstreuungserkennung: Proprietäres Homodyne-FSC-Sensorsystem mit Silizium-Fotodioden und eingebautem 488/8-nm-Bandpassfilter Bandpassfilter Enthält 13 neu positionierbare Filter: 450/45 660/10 (2) 525/40 (2) 690/50 585/42 712/25 610/20 (2) 780/60 (3) Streuauflösung: Blau (488 nm) Auflösung der Seitenstreuung: <300 nm Violett (405 nm) Seitenstreuungsauflösung (VSSC): 80 nm Polystyrol-Partikel Die Streuleistung ist für die Auflösung von menschlichen Lymphozyten, Monozyten und Granulozyten sowie von Nanopartikeln optimiert. Fluoreszensauflösung: Das CytoFLEX-Durchflusszytometer ist in der Lage, einen rCV von 3 % zu erreichen, wobei die Partikel zur Überprüfung der Ausrichtung einen rCV von <3 % erreichen. Probenflussraten: Feste Durchflussraten: 10, 30 und 60 μL/min Benutzerdefinierter Durchflussratenregelungsmodus von 10 bis 240 μL/min in 1 μL-Schritten. Gravimetrische Kalibrierung für absolute Zählungen innerhalb der CytExpert Software. Sonstige Daten: Spannung: 100-240 V Leistung: 150-250 W Maße: 42.5 cm x 42.5 cm x 34 cm Gewicht: 23,4 kg

The offered instrument / system is fully functional tested and ready to use. FURTHER DETAILS TO FOLL (more)OW

The offered instrument / system is fully functional tested and ready to use. FURTHER DETAILS TO FOLL (more)OW

The FACSCanto II system is an easy-to-use benchtop analyzer that delivers proven performance, accura (more)cy, and high-quality results. The FACSCanto II can be configured with two or three lasers to detect up to eight colors. It features many innovations, including a true fixed alignment flow cell to minimize startup time and improve  reproducibility. The optical system maximizes signal detection and increases sensitivity and resolution for each color in a multicolor assay. These and other capabilities make the BD FACSCanto II ideal for today’s busy clinical lab, providing a high degree of automation and quality control. With optimal reproducibility, the FACSCanto II system reduces hands-on technician time and costs associated with repeat testing. Lasers: 405-nm solid state diode, 30-mW fiber power output 488-nm solid state, 20-mW laser output 633-nm HeNe, 17-mW laser output Laser Configuration: Spatially separated beams with 9 x 65- μm elliptical spots Optical Alignment Procedure: Fixed, no operator alignment required Flow Cell: 180 x 430-μm rectangular quartz flow cell Collection Optics: Optical-gel coupled 1.2 NA lens FSC Resolution: 1.0 μm SSC Resolution: 0.5 μm Fluorescence Detector Design: Reflective optics with single transmission filter in front of each PMT FSC Detector: Photodiode with 488/10 BP SSC Detector: PMT with 488/10 BP Fluorescence Detectors: 8 PMTs in 4-2-2 configuration Violet Laser Dyes: Pacific Blue, AmCyan (455, 488 nm) Blue Laser Dyes: FITC, PE, PerCP or PerCP-Cy 5.5, PE-Cy 7 (525, 575, 678 or 695, 785 nm) Red Laser Dyes: APC, APC-Cy7 (660, 785 nm) Detector Bands: Violet Laser: 450/50; 502 to 525 nm Blue Laser: 530/30; 585/42; >670; 780/60 nm Red Laser: 660/20; 780/60 nm Fluorescence Threshold Sensitivities: FITC <100 MESF; PE <50 MESF Max Particle Size: 50 μm Sample Flow Rate: 10 μL/min to 120 μL/min Sample Acquisition Rate: 10,000 events/second, 6 compensated fluorescence parameters and 2 scatter parameters Sample Dead Volume: 30 μL (BD Falcon tubes 12 x 75-mm) Dimensions (DxWxH): Cytometer: 610 x 910 x 640 mm Fluidics cart: 610 x 790 x 640 mm Weight: Cytometer: 145 kg Fluidics cart: 51 kg

This listing is for the Becton Dickinson FACSCalibur Flow Cytometer. It is being sold as the unit on (more) its own without cables or software, it needs its laser replaced, otherwise it is in full working condition.The Becton Dickinson FACSCalibur Flow Cytometer offers aunique approach to flow cytometry, allowing users to perform both cell analysisand cell sorting in a single system, providing flexibility for a variety ofresearch and clinical applications. The BD FACSCalibur is a fully integratedmultiparameter system that has the performance and sensitivity to ensureobjective and reproducible results through the use of dual-laser technology,interbeam compensation, and an alignment-free optical design. Efficientcollection optics, coupled to the flow cell with an optical gel, permit the useof a low-powered, air-cooled argon laser and a red diode laser to ensure thehigh sensitivity required for analysis of low-density antigens.

Lasers: Blue Solid State: 488 nm, 55 mW, Software Controlled Red Solid State Diode: 638 nm, 50 mW, S (more)oftware Controlled Violet Solid State: 405 nm, 80 mW, Software Controlled Configuration: Nominal 90 µm spatially separated beam spots Flow cell: 430 µm x 180 µm rectangular channel Collection optics: Fixed integrated optics and quartz flow cell design with a numerical aperture of > 1.2 Detector filters: Forward Scatter: 488/20 Blue Laser: 525/40, 575/30, 614/20 , 675/20, 695/30, 755LP Dyes: FITC, PE, ECD, PC5 or PEC5.5, PECy7 Red Laser: 660/20, 725/20, 755 LP Dyes: APC or Alexa Fluor 647, APC-Alexa Fluor 700, APC-Cy7, APC-Alexa Fluor 750 Violet Laser: 450/50, 550/40 Dyes: Pacific Blue, Krome Orange Forward Scatter Detector: Fourier design providing up to 3 measurements of forward angle Side Scatter Detector: High performance photodiode with electronic attenuation Fluorescence Detectors: FL1 – FL6 Fluorescent Detectors Flow rates: Continuous pressure is applied to the sample tube based on user selected flow rates: Low, Medium and High Performance: Carryover: < 0.1%, Dead volume as low as 2 µL when using Beckman Coulter 12 x 75 mm polypropylene tube and adjustable probe Acquisition modes: 32 tube Multi Carousel Loader (MCL), Single tube sampling mode, Automated work list acquisition, Manual work list mode Mixing: The MCL vortexes each tube individually before sample acquisition Barcode reading: Carousel number, tube location, and tube barcode verified to worklist Biosafety: Biohazard contained wash station thoroughly rinses sample probe Throughput: 10000 normal leukocytes is 82 tubes/hour Scatter resolution: Resolves 0.4 micron particles from background noise using Forward Scatter, Maximum detection of up to 40 microns Fluorescence sensitivity: FITC < 107 MESF, PE < 64 MESF, PC5 < 13 MESF Acquisition rate: 25000 events per second, 90% yield Cytometer dimensions (WxHxD): 950 x 610 x 730 mm Cytometer weight: 104 kg Supply cart dimensions (WxHxD): 724 x 298 x 495 mm Supply cart weight: 30 kg Software included but requires a new licence purchase.

The Agilent xCELLigence Real-Time Cell Analysis (RTCA) SP instrument uses label-free cellular impeda (more)nce to continuously monitor cell health, behavior, and function with high accuracy, sensitivity, and reproducibility. The SP model can run a 96-well electronic microplate (E-Plate 96), which is ideal for cell health characterization, immune cell-mediated killing, viral cytopathic effects, cytotoxicity, cell adhesion, cell barrier function (TEER), cell signaling (e.g., GPCR) and other related applications. xCELLigence RTCA SP model can run one 96-well plate Measure cell health, viability, or response to treatments with high sensitivity, accuracy, and reproducibility Obtain continuous data at high temporal resolution (from seconds to days), with non-invasive and label-free real-time impedance technology Easy workflow allows users to simply add cells to E-plates and begin kinetic measurements at physiological conditions, with minimal hands-on time Intuitive RTCA Software Pro allows for analysis of real-time data to generate diverse plot types and automatically calculate parameters such as % of cytolysis, IC50 or KT50 RTCA Software Pro supports FDA 21 CFR Part 11 compliance to ensure electronic data integrity

Met One offers battery-operated portability, up to six size channels and a built-in printerall in on (more)e simple, compact instrument. The Met One 237 counts airborne particles in up to six different size ranges simultaneously. Count data is displayed on the front-panel as total number of particles (cumulative count) or the particles in each size range (differential count). The bright LED display lets you see the data easily and the printout shows cumulative and differential count data for each size range. Channel Sizes: 2 ch; 0.5 µm, 5.0 µm – 4 ch; 0.5 µm, 0.7 µm, 1.0 µm, 5.0 µm – 5 ch; 0.5 µm, 0.7 µm, 1.0 µm, 2.0 µm, 5.0 µm – 6 ch; 0.5 µm, 0.7 µm, 1.0 µm, 2.0 µm, 3.0 µm, 5.0 µm Flow Rate: 0.1 CFM (2.83 LPM) Coincidence Limit: 2 million/ft3 Light Source: Laser diode Data Storage: 500 samples, rotating buffer Sample and Hold Times: 1 second to 24 hours Count Alarms: 1 to 9,999,999, counts each channel Count Modes: Manual, auto, concentration, beep Concentration Mode: 1 second samples, 1 to 10 samples averaged, display updated every second; displays counts/ft3, liter or counts/1000L (m3) Battery Recharge Time: 16 hours (with unit off) Battery Operating Time: 4 hours with printing, 8 hours without printing Dimensions (WxhXD): 170 x 120 x 300 mm Weight: 3.1 kg

The HORIZON system from Halo Labs employs Backgrounded Membrane Imaging (BMI) technology to measure  (more)and characterize subvisible particles in a high-throughput, low volume format. BMI has its roots in membrane microscopy – a USP lot release method that images particles on a membrane surface. The fully automated, fluidics-free HORIZON system utilizes disposable membrane plates and automated image processing to rapidly generate reliable and accurate particle counts and sizing. It can perform particle characterization in up to 96 samples in a single run using as little as 25 µL per sample. BMI is also insensitive to solution refractive index, enabling reliable measurement of translucent protein aggregates. The HORIZON system uses sophisticated imageprocessing techniques to analyze images and acquire particle data. The key is to first take a background image of the membrane. After samples are filtered through by vacuum and the particles are captured, the same membrane is re-imaged with sample particles on the surface. The sample image is precisely aligned with the background image and then processed pixel by pixel so that the background texture is eliminated, and particles are viewed in high contrast. Backgrounding the image leads to high reproducibility and minimal interference from membrane texture and imperfections.

Partec Cyflow Space FACS stand alone system with Analytical cuvette. Equipped with: 4 measurable par (more)ameters: FCS, SSC, D3 and D4 bandpass filters (expandable to 9 parameters). Blue diode laser 20 mW 488 nm (expandable to 3 laser light sources). Sorting unit with green laser (sorting cuvette not included, but can be ordered from Sysmex). Fujitsu PC with Flowmax software for flow data analysis. Bottle rack for 1 liter bottles (for e.g. sheat fluids, buffers and waste).

The UF-100 is a fully automated urine cell analyzer using flow cytometry method combined with fluore (more)scent dye and electric resistance method to detects casts. This unit is further automated tham UA-1000/2000. All the process from mixing and aspirating of sample to output of analyzed parameters are performed automatically. Analysis includes quantitative analysis and flagging. Quantitative analysis measures: RBC, WBC, epithelial cells, casts and bacteria count (concentration) in the unit of µL or view field. Flagging marks: Path, CAST (casts containing organized structures),  SRC (small round epithelial cell), YLC (yeast liked fungi cell), X’TAL (crystal), SPERM (spermatozoa) and outputs the flagging informarion (information of their presence). Analyzed results are displayed on screen and if required printed by card type printer of color printer, and they are transmitted to a host computer. Dimensions (WxHxD): 600 x 705 x 606 mm Weight: 68 kg

Imaging performance Cell size: 5 – 300 microns (Cellometer CHT4-PD300 Slides are required for cells  (more)> 80 microns in diameter) Concentration range: 105 – 107 cells/ml Bright field imaging, fluorescent imaging and pattern-recognition software to quickly and accurately decluster, identify and count individual cells Fluorescent optics Excitation/Emission: 470nm/535nm Example fluorophores: Acridine Orange (+DNA) Calcein AM CFDA SYTO 9 SYTO 13 Excitation/Emission: 540nm/605nm Example fluorophores: Propidium iodide Ethidium bromide Dimensions (WxDxH): 280 x 300 x 330 mm Weight: 11.8 kg

Address the full range of current and future multi-parameter cellular analysis research needs with t (more)he NovoCyte flow  ​cytometer. ACEA brings researchers high performance flow cytometry at a low investment cost with the NovoCyte platform. Lasers: Blue 488 nm, Red 640 nm Detectors: 530, 585, 675 nm Laser Configuration: Spatially separated beams with 10 x 80 μm elliptical spots Optical Alignment Procedure: Fixed, no operator alignment required Flow Cell: 170 x 290 μm rectangular quartz flow cell FSC/SSC Resolution: FSC < 0.5 μm, SSC < 0.2 μm Cell Size: 0.2 – 50 μm Fluorescence Threshold Sensitivity: FITC < 75 MESF, PE < 50 MESF Fluorescence Resolution: < 3% CV for CEN Filters: User Exchangeable Sample Acquisition Rate: 35,000 events/second Volumetric Absolute Count Precision: CV < 5% Sample Flow Rate: 5 – 120 μL/min Sheath Flow Rate: 6.5 mL/min Sample Aspiration Volume: 10 – 100 μL Fluid Container Capacity: 3 L sheath, 3 L waste, 500 mL cleaning, 500 mL decontamination Carryover: < 0.1% Fluidics Maintenance: Automated startup, cleaning, decontamination and shutdown Manual Sample Loading: 12 x 75 mm tube Dimension (WxDxH): 600 x 450 x 390 mm Weight: 39 k

Coulter ZM Cell Counter, &nbsp;S.ST.II Sampling Stand &amp; V.C.U. VWII Vacuum Container This syste (more)m was removed from a university lab where it was surplus to requirements. Included are; 1x Coulter Counter ZMModel No. Z.M .The unit powers on and is in good cosmetic condition. There are some tape marks from general use. All knobs, dials and buttons are responsive to the unit.Both digital LCD screens are working and display well. 1x Coulter Sampling StandModel No. S.ST.IIWe were unable to power up the unit. Both the fuses were fine. 1x Coulter VW iiModel VCUThe unit is in good cosmetic condition, it has 2x 500ml glass jars with lids housed inside it. These lids have 2x hoses attached to them. Manuals:9x manuals are in good cosmetic condition and appear to be complete1x Datasheet book.

2 x Becton-Dickinson Remote Pre Amp - BD Biosciences LSR II Boards These were removed from a univers (more)ity lab where they were surplus to requirements. They are in good cosmetic condition but have not been tested.

BD FACSCalibur Flow Cytometer This was removed from a university lab where it was surplus to require (more)ments. The unit has a main board issue and is sold for spares/repairs

Beckman Coulter Z1 Particle Counter with Controller This was removed from a university lab where it  (more)was surplus to requirements. The unit powers up but has not been tested further. it is in good cosmetic condition with minor marks.

Beckman Coulter MoFlo XDP Cell Sorter Laser Cytomation System + Optics Table Lab This system was rem (more)oved from a university where it was surplus to requirement. It is in good cosmetic condition, the working condition of each unit that we were able to test is listed below. Parts of the system:- Optics Table - MoFlo Optical BenchSN - 2086Dimensions - L153 W122 H80.5cmMeasurement - Imperial Parts fitted on table:- Pressure Console UnitSN - 2086 Coherent SapphireModel - Sapphire LP USB CDRH Driver Unit w/displayPN - 1189089SN - LDP.1189089.C02070- Powers on Coherrent Sapphire 488-200 CW CDRHPN - 1137970SN - LDP.1137970.521683Heatsink Sapphire LP RoHS compl- NOT TESTED Coherrent CubePN - 1170509SN - C20010803- NOT TESTED Coherrent CubePN - 1193821/AASN - C14070901- NOT TESTED Hamamatsu Parts:-H957-27 (No.T0050)H957-18 (No.K0149)H957-27 (No.T3721)H957-25 (No.S0319)H957-25 (No.S0360)H957-25 (No.S0359)H957-25 (No.S0322)H957-25 (No.S0324)H957-27 (No.T0051)H957-27 (No.T0054)H957-25 (No.S0358)H957-25 (No.S0321)H957-25 (No.S0323) Smart Sampler 2 x Preamplifier UnitsSN - 2086 DTx Data Display 19" LCD w/TouchscreenBCI PN - B44222DTx PN - 90819SN - BCM0514F37003- Powers on via Miami MoFlo XDP Controller Under table units:- DTx BCI Miami MoFlo XDP ControllerDTx PN - 90692BCI PN - B20566DTx SN - BCM0915F44602- Powers on - When powered up, an error message reads 'OdinInstControl::connectMachine failed: AttachedInstrument::GetInstance:Repository had communication error!' Gast Vacuum PumpDOA-P501-BN- Powers on Intellipower UPSModel - IQ200V-C-DiAC-Isol-PFCSN - 2FKVDIFC5B15110524- Powers on Beckman Coulter Aerosol Evacuation System Buffalo Filter w/ PedalModel - 200-0179SN - 502135Has ViroSafe Filter fitted (PN-200-8166)- Powers on. 3 x 316L UM Alloy Products Corp Pressure Vessels:-SN - S533735-1-005 (2014)SN - S514233-1-011 (2010)SN - S507165-1-008 (2008) Other units:- Thermo Scientific Recirculating Water BathControl - C10 Type - 003-5009/SN - 1 08004133017Bath - K20 Type - 002-4276/SN - 08004052 003- Powers on Testing:-37˚c - Thermometer read 37.6˚c100˚c - Thermometer read 100.0˚c-20˚c - Thermometer didn't read lower than -13.5˚c Jun Air CompressorType - OF302-25MQSN - 0119802962- Powers on with compressor running Hours - 00898.3 Other spare bits:-1 x Black Case of various filters1 x Black Case containing 3 x Merlin Gerin fuses, Methanol &amp; Acetone2 x Sets of Spare Shaped Charged Plates (one pair boxed)1 x Box of Filters1 x Box of mixed size O-ringsRed Ethernet cable5 x IEC Extension cables

MoFlo Astrios EQS Cell Sorter in BSL Hood. All units are in good used cosmetic condition. The system (more) powers up as it should. We were informed by the lab that the Red laser is faulty and requires replacement. The unit has the following lasers installed: 1 X 640NM - 106MW.1 X 561NM - 202MW.1 x 488NM - 200MW.1 X 405NM - 55MW. The writing is quite faint on the MW output, so the above figures might not be 100% correct. The complete system consists of: 1 x Baker Company SG503HE-BC-INT SterilGARD hood.Manufactured 09/30/2013. 1 x Moflo Astrios EQS Main Unit.The unit has various marks and scratches from general use.Powers up fine. 1 x Moflo Astrios Lower/Laser/Computer Section.The laser &amp; computer system powers up fine. 1 x Thermo/Haake SC100/A28 re-circulator/chiller.Powers up fine. 1 x Intellipower UPS.Powers up fine. 1 x Jun-Air OF302 Silent Air Compressor.It was noted by the lab that this is quite loud. We powered it on and it sounds normal for one of these units. Also includes various spares and accessories shown in pictures.3 x '100' spare nozzle tips8 x '70' spare nozzle tips.

Beckman Coulter MoFlo Astrios EQS Flow Cytometer System &amp; Sterilgard Cabinet This was removed f (more)rom a university lab where it was surplus to requirements. The PC with the system cannot run the software. The software is included but will require a Beckman OEM PC system to run. The unit has the following lasers installed: 1 X 640NM - 106MW.1 x 592NM - 200MW.1 X 561NM - 202MW.1 x 532NM - 150MW.1 x 488NM - 200MW.1 X 405NM - 55MW. The writing is quite faint on the MW output, so the above figures might not be 100% correct. The complete system consists of: 1 x Baker Company SG503HE-BC-INT SterilGARD hood.Manufactured 04/13/2015.The unit has been powered up. 1 x Moflo Astrios EQS Main Unit.The unit has various marks and scratches from general use. It powers up fine. 1 x Moflo Astrios Lower/Laser/Computer Section.The laser &amp; computer system power up fine.

Beckman Coulter Z1 Particle Counter with Controller This was removed from a university lab where it  (more)was surplus to requirements. The unit powers up but has not been tested further. it is in good cosmetic condition with minor marks.

Biotest APC M3 P52100S Airborne Particle Counter and APC Plus 943201 These items were removed from a (more) university where they were surplus to requirement. Both items are in good cosmetic condition and power up, we are unable to test them further at our facility. The following items are included: APC M3Model P52100SSerial No. F0708-010310Year 2008 APC PlusModel 943201Serial No. B0408-2275Year 2008 Boxer Custom CaseSerial No. 13665External 44cm x 52cm x 39cmInternal 39cm x 47cm x 20cm Accessories included:1 x power lead1 x 7-volt power transformer supply lead1 x stainless suction cup with 1 meter hose

Beckman Coulter Particle Counter &amp; Size Analyser Z2 with Controller This was removed from a uni (more)versity lab where it was surplus to requirements. The unit powers up but we were unable to test it further without a diluted solution. It is in good cosmetic condition with minor marks from use.

Beckman/MoFlo Optical Imperial TableModel: 1422Manufactured: Nov 2005 This was removed from a univer (more)sity lab where it was surplus to requirements. The unit has not been tested but is in reasonably good cosmetic condition. The table has imperial 1-inch spacing.