HPLC, Liquid Chromatography

System consists of: 1x Agilent 1100 solvent tray 1x Agilent 1100 degasser type G1379A 1x Agilent 110 (more)0 quaternary pump type G1311A 1x Agilent 1100 autosampler type G1313A 1x Agilent 1100 column compartment type G1316A 1x Agilent 1100 variable wavelength detector type G1314A

Programmable flow rate: 0.00 to 5.00 mL/min, 0.01 mL/min increments Maximum operating pressure: 401  (more)bar Pressure ripple: <2% at 0.5mL/min Flow precision: 2 seconds SD Flow accuracy: 2% of setting Gradient accuracy: <1% between 10 to 90% Gradient precisison: <0.5% RSD between 10 to 90% Gradient delay volume: <60 µL (without mixer), <200 µL (with one 50 µL mixer) Inline degasser channels: 2 Dimensions (HxDxW): 430 x 610 x 305 mm Weight: 27.2 kg

Agilent 1100 system consisting of: 1x Agilent 1100 Degasser type G1379A 2x Agilent 1100 Isocratic Pu (more)mp type G1310A 1x Agilent 1100 Variable Wavelength Detector type G1314A 1x Agilent 1100 Fluorescence Detector type G1321A 2x Solvent tray Manual injector with 1 ml sample loop Laptop Agilent 1100 IsoPump: Hydraulic system: Dual piston in series pump with proprietary servo-controlled variable stroke drive, floating pistons and active inlet valve Setable flow range: 0.001 – 10 ml/min, in 0.001 ml/min increments Flow range: 0.2 – 10.0 ml/min Flow precision: < 0.3 % RSD (typically 0.15 %), based on retention time, at 1 ml/min Pressure: Operating range 0 – 40 MPa (0 – 400 bar, 0 – 5880 psi) up to 5 ml/min, Operating range 0 – 20 MPa (0 – 200 bar, 0 – 2950 psi) up to 10 ml/min Pressure pulsation: < 2 %amplitude (typically < 1 %), at 1 ml/min isopropanol, at all pressures > 10 bar (147 psi) Compressibility compensation: User-selectable, based on mobile phase compressibility Recommended pH range: 1.0 – 12.5, solvents with pH > 2.3 should not contain acids which attack stainless steel Dimensions (HxWxD): 140 x 345 x 435 mm Weight: 11 kg Agilent 1100 VWD: Detection type: Double-beam photometer Light source: Deuterium lamp Wavelength range: 190–600 nm Short term noise (ASTM): ± 0.75 x 10-5 AU at 254 nm Drift: 3 x 10-4 AU/hr at 254 nm Linearity: > 2 AU (5%) upper limit Wavelength accuracy: ± 1 nm Self-calibration with deuterium lines, verification with holmium oxide filter Band width: 6.5 nm typical Flow cell: 14-µl volume, 10-mm cell path length and 40 bar (588 psi) pressure maximum Dimensions (HxWxD): 140 x 345 x 435 mm Weight: 11 kg Agilent 1100 FLD: Detection type: Multi-signal fluorescence detector with rapid on-line scanning capabilities and spectral data analysis Light source: Xenon flash lamp Pulse frequency: 296 Hz for single signal mode, 74 Hz for spectral mode Excitation monochromator: range; 200 – 700 nm and zero-order,  bandwith; 20 nm, monochromator; concave holographic grating F/1.6 blaze 300 nm Emission monochromator: range; 280 – 900 nm and zero-order,  bandwith; 20 nm, monochromator; concave holographic grating F/1.6 blaze 400 nm Spectrum acquisition: Excitaiotn or emmision spectra, scan  speed; 28 ms per data point, step size; 1 – 20 nm Wavelength characterisric: Repeatability; ±0.2 nm, accuracy; ±3 nm Flow cell: 8 µl volume and 20 bar pressure, quartz Dimensions (HxWxD): 135 x 345 x 435 mm Weight: 11.5 kg

TSP HPLC P4000 + AS3000

System consists of: Agilent G1322A Degasser Agilent G1311A Quaternary Pump Agilent G1313A Autosample (more)r Agilent G1316A Thermostatted Column Compartment Agilent G1314A Variable Wavelength Detector

System consisting of: Gilson GX-281 sample robot 2 Gilson 6 position valves 2 Gilson 306 pump Gilson (more) 322 pump

System consisting of: Gilson GX-281 sample robot 2 Gilson 6 position valves Gilson 306 pump Gilson 1 (more)59 UV-Vis detector Gilson 321 pump Software

System contains: 2 Solvent cabinets 2 Capillary pumps 1 Well-plate samplers 2 Autosampler thermostat (more)s 1 Thermostatted colomn compartment 1 Diode array detector 1 Handheld control module Vacuum degasser cel is missing in the vacuum degasser Capillary pump: Settable column flow range: 0.01 – 20 µl/min Recommended column flow range: 1 – 20 µl/min Column flow precision: < 0.7 % RSD or 0.03 % SD (typically 0.4 % RSD or 0.02 % SD), at 10 µl/min and 50 µl/min column flow (based on RT, default setting) Optimum composition range: 1 to 99% or 5 µl/min per channel (primary flow), whatever is greater Composition precision: < 0.2 % SD, at 10 µl/min (20 µl flow sensor), 50 µl/min (100 µl flow sensor) and 1 ml/min (normal mode) default setting Delay volume: Typically 3 µl from the electronic flow control to the pump outlet for flow rates up to 20 µl/min. Pressure range: 20 to 400 bar (5880 psi) system pressure Compressibility compensation: User-selectable, based on mobile phase compressibility Recommended pH range: 1.0 – 8.5, solvents with pH < 2.3 should not contain acids which attack stainless steel. Upper pH range is limited by fused silica capillaries. Well-plate sampler: Safety features: Leak detection and safe leak handling, low voltages in maintenance areas, error detection and display Injection range: 0.01 – 8 µl in 0.01 µl increments Precision: Typically < 0.5 % RSD of peak areas from 5 – 40 µl, Typically < 1 % RSD from 1 – 5 µl, Typically < 3 % RSD from 0.2 – 1 µl Sample viscosity range: 0.2 – 5 cp Sample capacity: 2 × well-plates (MTP) + 10 × 2 ml vials, 100 x 2 ml in one tray, 40 x 2 ml in half tray Injection cycle time: Typically < 30 s using following standard conditions: Default draw speed: 4 µl/min, Default eject speed: 10 µl/min, Injection volume: 0.1 µl Carry-over: Typically < 0.05 % using the following conditions: Column: 150 x 0.5 mm Hypersil ODS, 3 µm Mobile phase: Water/Acetonitrile = 85/15 Column Flow rate: 13 µl/min Injection volume: 1 µl caffeine (=25ng caffeine), 1 µl water to test carryover. Outside wash of needle before injection: 20 sec with water using flush port Autosampler thermostat: Temperature range: setable from 4 °C to 40 °C in 1 ° increments Temperature accuracy at ambient temperatures < 25 °C and humidity < 50%: – 1°C to + 4 °C at a setpoint of 4 °C Temperature accuracy at ambient temperatures > 25 °C and/or humidity > 50%: – 1°C to + 5 °C at a setpoint of 4 °C Thermostatted colomn compartment: Temperature range: 10 degrees below ambient to 80 °C, up to 80 °C: flow rates up to 5 ml/min Temperature stability: ± 0.15 °C Temperature accuracy: ± 0.8 °C (± 0.5 °C With calibration) Column capacity: Three 30 cm Warm-up/cool-down time: 5 minutes from ambient to 40 °C, 10 minutes from 40 – 20 °C Dead volume: 3 µl Communications: Controller-area network (CAN), GPIB, RS-232C, APG Remote: ready, start, stop and shut-down signals, LAN via other series module Safety and maintenance: Extensive diagnostics, error detection and display (through control module and Agilent ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas. Diode array detector: Detection type: 1024-element photodiode array Light source: Deuterium and tungsten lamps Wavelength range: 190 – 950 nm Short term noise (ASTM) Single and Multi-Wavelengt: ± 1 × 10-5 AU at 254 and 750 nm Drift: 2 × 10-3 AU/hr at 254 nm Linear absorbance range: > 2 AU (upper limit) Wavelength accuracy: ± 1 nm Self-calibration with deuterium lines, verification with holmium oxide filter Wavelength bunching: 1 – 400 nm Programmable in steps of 1 nm Slit width: 1, 2, 4 , 8, 16 nm Programmable slit Diode width: < 1 nm Flow cells: 13 µl volume, 10 mm cell path length and 120 bar (1760 psi) pressure maximum Control and data evaluation: Agilent ChemStation for LC Analog outputs: Recorder/integrator: 100 mV or 1 V, output range 0.001 – 2 AU, two outputs Communications: Controller-area network (CAN), GPIB, RS-232C, APG Remote: ready, start, stop and shut-down signals, LAN optional Safety and maintenance: Extensive diagnostics, error detection and display through control module and ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas. GLP features: Early maintenance feedback (EMF) for continuous tracking of instrument usage in terms of lamp burn time with user-settable limits and feedback messages. Electronic records of maintenance and errors. Verification of wavelength accuracy with built-in holmium oxide filter. Handheld control module: The control module provides complete local control and monitoring of a single module or an entire Agilent series system. There is no data evaluation in the control module. The control module allows you to do a variety of HPLC tasks including automated sample preparation and injection, isocratic, gradient and multiple method analyses.

Solvent delivery method: Parallel-type double plunger Plunger capacity: 10µL Maximum discharge press (more)ure: 40MPa Flow-rate setting range: 0.0001 to 10.0000 mL/min Flow-rate accuracy: No more than 1% or 0.5µL/min, whichever is greater (0.01 to 2 mL/min.) Flow-rate precision: No more than 0.06% RSD or 0.02 min SD, whichever is greater Pulsation: 0.1 MPa (for water at 1.0 mL/min. and 7MPa) Constant-pressure solvent delivery: Supported Plunger rinsing mechanism: Manual rinsing or automatic rinsing using optional product Safety measures: Liquid-leakage sensor, high-pressure/low-pressure limits Gradient type: Low-pressure mixing Number of mixed solvents: 4 max Dimensions (WxHxD): 260 x 140 x 420 mm Weight: 10 kg

Ultra high performance means the highest resolution, highest sensitivity and fastest analysis – in o (more)ther words, a superlative LC system. Flexar FX-15 is the ultimate in UHPLC with maximum throughput and minimum stress for you. Getting more sample information in less time lets you be more productive.

Autosampler: Isocratic pump:

System contains: Solvent tray Vacuum degasser: G1322A Vacuum degasser: The Agilent 1100 HPLC Degasse (more)r features a 4-channel vacuum container, including four tubular plastic membranes, and a vacuum pump. This degasser has a maximum flow rate of 0-10ml/min flow rate per channel and 5-10ml/min at reduced degassing performance. Quaternary pump: Hydraulic system: Dual plunger in series pump with proprietary servo-controlled variable stroke drive, floating plungers and active inlet valve Setable flow range: 0.001 – 10 ml/min, in 0.001 ml/min increments Flow range: 0.2 – 10.0 ml/min Flow precision: < 0.07% RSD, or < 0.02 min SD whatever is greater, based on retention time at constant room temperature Flow accuracy: ± 1% or 10 µl/min whatever is greater Pressure: Operating range 0 – 400 bar up to 5 ml/min, 0 – 200 bar up to 10 ml/min Pressure pulsation: < 2 % amplitude (typically < 1 %), at 1 ml/min isopropanol, at all pressures > 10 bar Compressibility compensation: User-selectable, based on mobile phase compressibility Recommended pH range: 1.0 – 12.5, solvents with pH < 2.3 should not contain acids which attack stainless steel Gradient formation: Low pressure quaternary mixing/gradient capability using proprietary high-speed proportioning valve Delay volume 800 – 1100 µl, dependent on back pressure Composition Range: 0 – 95 % or 5 – 100 %, user selectable Composition Precision: < 0.2 % RSD, at 0.2 and 1 ml/min Autosampler thermostat: Temperature range: setable from 4°C to 40°C in 1°C increments Temperature accuracy (at ambient temperatures < 25 °C and humidity < 50%): – 1°C to + 4°C at a setpoint of 4°C Temperature accuracy (at ambient temperatures > 25 °C and/or humidity > 50%): – 1°C to + 5°C at a setpoint of 4°C Standard autosampler: Pressure: Operating range 0 – 400 bar GLP features: Early maintenance feedback (EMF), electronic records of maintenance and errors Safety features: Leak detection and safe leak handling, low voltages in maintenance areas, error detection and display Injection range: 0.1 – 100 µl in 0.1 µl increments Replicate injections: 1 – 99 from one vial Precision: <0.25% RSD from 5 – 100 µl, <1% RSD 1 – 5 µl Minimum sample volume: 1 µl from 5 µl sample in 100 µl microvial, or 1 µl from 10 µl sample in 300 µl microvial Carryover: Typically <0.1% Sample viscosity range: 0.2 – 50 cp Replicate injections per vial: 1 – 99 Sample capacity 100 x 2 ml vials in 1 tray Injection cycle time: Typically 50 s depending on draw speed and injection volume Thermostatted colomn compartment: Temperature range: 10 degrees below ambient to 80 °C, up to 80 °C: flow rates up to 5 ml/min Temperature stability: ± 0.15 °C Temperature accuracy: ± 0.8 °C (± 0.5 °C With calibration) Column capacity: Three 30 cm Warm-up/cool-down time: 5 minutes from ambient to 40 °C, 10 minutes from 40 – 20 °C Dead volume: 3 µl Communications: Controller-area network (CAN), GPIB, RS-232C, APG Remote: ready, start, stop and shut-down signals, LAN via other series module Safety and maintenance: Extensive diagnostics, error detection and display (through control module and Agilent ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas. Diode array detector: Detection type: 1024-element photodiode array Light source: Deuterium and tungsten lamps Wavelength range: 190 – 950 nm Short term noise (ASTM) Single and Multi-Wavelengt: ± 1 × 10-5 AU at 254 and 750 nm Drift: 2 × 10-3 AU/hr at 254 nm Linear absorbance range: > 2 AU (upper limit) Wavelength accuracy: ± 1 nm Self-calibration with deuterium lines, verification with holmium oxide filter Wavelength bunching: 1 – 400 nm Programmable in steps of 1 nm Slit width: 1, 2, 4 , 8, 16 nm Programmable slit Diode width: < 1 nm Flow cells: 13 µl volume, 10 mm cell path length and 120 bar (1760 psi) pressure maximum Control and data evaluation: Agilent ChemStation for LC Analog outputs: Recorder/integrator: 100 mV or 1 V, output range 0.001 – 2 AU, two outputs Communications: Controller-area network (CAN), GPIB, RS-232C, APG Remote: ready, start, stop and shut-down signals, LAN optional Safety and maintenance: Extensive diagnostics, error detection and display through control module and ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas. GLP features: Early maintenance feedback (EMF) for continuous tracking of instrument usage in terms of lamp burn time with user-settable limits and feedback messages. Electronic records of maintenance and errors. Verification of wavelength accuracy with built-in holmium oxide filter.

The 515 HPLC pump is a programmable, free-standing HPLC pump available in isocratic, gradient, and s (more)emi-preparative configurations: Precise stepper motor control (48 steps/µL resolution) of dual reciprocating pistons Non-circular drive gears provide smooth, uniform eluent delivery User-programmable flow rate calibration and compressibility compensation factors ensure straightforward pump operation qualification Tests to retention time variations of less than 0.1% for highly reproducible peak performance Easy-to-read display and keypad for error-free programming Automatic purging and flow calibration

System consists of: Jasco UV-1570M Intelligent UV Detector Jasco LG-1580-02 Ternary Gradient Unit Ja (more)sco PU-1580 Intelligent HPLC Pump Jasco AS-1555 Intelligent Sampler 2 Alltech Elite Degassing System PC