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Agilent Series 1100 HPLC System
System contains:
Solvent tray
Vacuum degasser
Binary pump
Autosampler
Thermostatted colomn compartment
Variable wavelength detector
Vacuum degasser:
The Agilent 1100 HPLC Degasser features a 4-channel vacuum container, including four tubular plastic membranes, and a vacuum pump. This degasser has a maximum flow rate of 0-10ml/min flow rate per channel and 5-10ml/min at reduced degassing performance.
Binary pump:
Flow Precision: <0.3 % RSD (typically <0.15 % RSD)
Flow Range: Setpoints from 0.001 to 5.0 ml/min, in 0.001 ml/min increments
Operating range: 0 – 40 MPa (0 – 400 bar, 0 – 5880 psi)
Pulsation: <2 % amplitude (typically <1 %) at 1 ml/min isopropanol at all pressures >1 MPa (> 10 bar, >147 psi)
Compressibility compensation: User selectable, based on mobile phase compressibility
Delay volume: 180 – 480 µl (600-900 µl with mixer) dependent on back pressure
Recommended pH range: 1.0 – 12.5. Solvents with pH <2.3 should not contain acids which attack steel
Well-plate autosampler:
Safety features: Leak detection and safe leak handling, low voltages in maintenance areas, error detection and display
Injection range: 0.01 – 8 µl in 0.01 µl increments
Precision: Typically < 0.5 % RSD of peak areas from 5 – 40 µl, Typically < 1 % RSD from 1 – 5 µl, Typically < 3 % RSD from 0.2 – 1 µl
Sample viscosity range: 0.2 – 5 cp
Sample capacity: 2 × well-plates (MTP) + 10 × 2 ml vials, 100 x 2 ml in one tray, 40 x 2 ml in half tray
Injection cycle time: Typically < 30 s using following standard conditions: Default draw speed: 4 µl/min, Default eject speed: 10 µl/min, Injection volume: 0.1 µl
Carry-over: Typically < 0.05 % using the following conditions: Column: 150 x 0.5 mm Hypersil ODS, 3 µm
Mobile phase: Water/Acetonitrile = 85/15
Column Flow rate: 13 µl/min
Injection volume: 1 µl caffeine (=25ng caffeine), 1 µl water to test carryover. Outside wash of needle before injection: 20 sec with water using flush port
Thermostatted colomn compartment:
Temperature range: 10 degrees below ambient to 80 °C, up to 80 °C: flow rates up to 5 ml/min
Temperature stability: ± 0.15 °C
Temperature accuracy: ± 0.8 °C (± 0.5 °C With calibration)
Column capacity: Three 30 cm
Warm-up/cool-down time: 5 minutes from ambient to 40 °C, 10 minutes from 40 – 20 °C
Dead volume: 3 µl
Communications: Controller-area network (CAN), GPIB, RS-232C, APG Remote: ready, start, stop and shut-down signals, LAN via other series module
Safety and maintenance: Extensive diagnostics, error detection and display (through control module and Agilent ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas.
Variable wavelength detector:
Detection type: Double beam photometer
Light source: Deuterium lamp
Wavelength range: 190 – 600 nm
Wavelength accuracy: ± 1 nm
Bandwidth: 6.5 nm
Linearity:> 2AU (5%) upper limit
Flow cell standard: 14 µl volume, 10 mm cell path length, 40 bar
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