Agilent 1100 HPLC System

Agilent 1100 system consisting of:

1x Agilent 1100 Degasser type G1379A

2x Agilent 1100 Isocratic Pump type G1310A

1x Agilent 1100 Variable Wavelength Detector type G1314A

1x Agilent 1100 Fluorescence Detector type G1321A

2x Solvent tray

Manual injector with 1 ml sample loop

Laptop

Agilent 1100 IsoPump:

Hydraulic system: Dual piston in series pump with proprietary servo-controlled variable stroke drive, floating pistons and active inlet valve

Setable flow range: 0.001 – 10 ml/min, in 0.001 ml/min increments

Flow range: 0.2 – 10.0 ml/min

Flow precision: < 0.3 % RSD (typically 0.15 %), based on retention time, at 1 ml/min

Pressure: Operating range 0 – 40 MPa (0 – 400 bar, 0 – 5880 psi) up to 5 ml/min, Operating range 0 – 20 MPa (0 – 200 bar, 0 – 2950 psi) up to 10 ml/min

Pressure pulsation: < 2 %amplitude (typically < 1 %), at 1 ml/min isopropanol, at all pressures > 10 bar (147 psi)

Compressibility compensation: User-selectable, based on mobile phase compressibility

Recommended pH range: 1.0 – 12.5, solvents with pH > 2.3 should not contain acids which attack stainless steel

Dimensions (HxWxD): 140 x 345 x 435 mm

Weight: 11 kg

Agilent 1100 VWD:

Detection type: Double-beam photometer

Light source: Deuterium lamp

Wavelength range: 190–600 nm

Short term noise (ASTM): ± 0.75 x 10-5 AU at 254 nm

Drift: 3 x 10-4 AU/hr at 254 nm

Linearity: > 2 AU (5%) upper limit

Wavelength accuracy: ± 1 nm Self-calibration with deuterium lines, verification with holmium oxide filter

Band width: 6.5 nm typical

Flow cell: 14-µl volume, 10-mm cell path length and 40 bar (588 psi) pressure maximum

Dimensions (HxWxD): 140 x 345 x 435 mm

Weight: 11 kg

Agilent 1100 FLD:

Detection type: Multi-signal fluorescence detector with rapid on-line scanning capabilities and spectral data analysis

Light source: Xenon flash lamp

Pulse frequency: 296 Hz for single signal mode, 74 Hz for spectral mode

Excitation monochromator: range; 200 – 700 nm and zero-order,  bandwith; 20 nm, monochromator; concave holographic grating F/1.6 blaze 300 nm

Emission monochromator: range; 280 – 900 nm and zero-order,  bandwith; 20 nm, monochromator; concave holographic grating F/1.6 blaze 400 nm

Spectrum acquisition: Excitaiotn or emmision spectra, scan  speed; 28 ms per data point, step size; 1 – 20 nm

Wavelength characterisric: Repeatability; ±0.2 nm, accuracy; ±3 nm

Flow cell: 8 µl volume and 20 bar pressure, quartz

Dimensions (HxWxD): 135 x 345 x 435 mm

Weight: 11.5 kg

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