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The Synthetic Genomics BioXp 3200 DNA Printer System represents a significant advancement in gene assembly technology, designed to facilitate the rapid development of synthetic genomes and genome components for research and development purposes.
The BioXp 3200 System efficiently synthesizes high-quality, linear DNA fragments from custom-designed oligonucleotide pools and reagents, covering a broad spectrum of complexity. It is capable of assembling up to 32 DNA fragments or "Tiles" per run, with sizes ranging from 350 base pairs (bp) to 1.8 kilobases (kb), ensuring high fidelity in each assembly. The synthesized DNA fragments are ready for offline sequencing quality control, further assembly into larger constructs, and cloning into vectors with compatible ends, in addition to supporting a wide range of downstream applications.
Key Specifications:
- **Tile Size Range**: 350 – 1800 bp
- **Degenerate Library Tile Size Range (Recommended)**: 300 – 800 bp
- **Clone Size Range**: 350 – 1800 bp
- **GC Content Complexity**:
- Tiles: 20 – 70% GC for 350 bp – 1.0 kb, 40 – 60% GC for 350 bp – 1.8 kb
- Degenerate Library Tiles: 20 – 70% GC within the recommended size range
- Clones: 20 – 70% GC for 350 bp – 1.0 kb, 40 – 60% GC for 350 bp – 1.8 kb
- **Composition**:
- Tiles and Clones: A, T, C, or G residues
- Degenerate Library Tiles: IUPAC code residues
- **Yield**:
- Tiles: >200 ng of purified, linear double-stranded DNA in 45 µL TE Buffer
- Degenerate Library Tiles: >200 ng of purified, linear dsDNA library in 45 µL TE Buffer
- Clones: Approximately 20 µL of cloned product (± 2 µL) and approximately 40 µL of uncloned Tiles (± 2 µL)
- **Format**: 1 to 32 reactions per run in a 96-well plate format
Dimensions of the system are 660 mm in width, 530 mm in height, and 740 mm in depth, with a total weight of 63.4 kg. This second-hand BioXp 3200 system offers the reliability and accuracy expected by research professionals, providing a robust solution for synthetic biology applications.
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