Chromatography is a technique based on the fact that different substances can pass through a stationary system at different speeds. There are many different types of devices that use chromatography to analyse and separate substances.

Chromatography

Shimadzu Prominence HPLC system features 5-channel degasser, dual-plunger pump, precise column oven, and autosampler for molecular diagnostics.

The **Shimadzu Prominence HPLC System** is an integrated analytical solution widely used in biotechnology, molecular diagnostics, and bioprocessing for precise separation and quantification of chemical compounds. This system comprises:

- **DGU-20A5 Degasser**: Equipped with 5 channels, providing effective solvent degassing with an internal volume of 380 µL, operational within 4°C to 35°C to maintain solvent integrity during liquid chromatography workflows.  
- **LC-20AT Pump**: Features a serial double-plunger design ensuring continuous, pulse-free flow with a flow rate range of 0.001 to 10.000 mL/min, optimized for high-precision gradient elution critical in molecular biology and synthetic biology applications. The pump also includes improved bubble removal technology enhancing system reliability.  
- **CTO-20A Column Oven**: Offers precise temperature control from 10°C above ambient up to 85°C with ±0.1°C accuracy, accommodating up to 10 columns with maximum length of 25 cm. The temperature range supports biomolecule stability during chromatography separation processes essential in biopharmaceutical production pipelines.  
- **SIL-20AC ht Autosampler**: Enables injections from 0.1 to 100 µL volumes with a sample capacity of 70 vials (1.5 mL) plus 10 control vials (1.5 mL), facilitating high-throughput sample processing required in clinical diagnostics and live-cell imaging studies.

This system’s modular configuration and reliable performance make it critical for advanced analytical tasks in **biotechnology research**, including gene editing product analysis, bioprocessing quality control, and synthetic biology research. Its precision and versatility support highly reproducible chromatography data in biomanufacturing and molecular cloning workflows.

Shimadzu Prominence HPLC System with Degasser, Binary Pump, Column Oven, and Autosampler

Integrated Knauer HPLC system with 4-channel manager, diode array UV detector, double-piston pump, column thermostat, and autosampler.

The Knauer HPLC System comprises multiple modules optimized for precise chromatographic analysis:  

- **Smartline Manager 5000**:  
  - Independent channel control (1-4)  
  - Flow path degassing via fluoropolymer membrane, max flow 10 ml/min  
  - Degassing capacity approx. 2 ppm oxygen at 1 ml/min  
  - Pressure rating: 70 PSIG, dead volume ~480 µl per channel  
  - Materials contacting solvents: PEEK, glass-filled PTFE, Teflon AF  
  - Suitable solvents: universal except hydrochloric acid and halogenated carbohydrates (e.g., HFIP)  

- **Smartline UV Detector 2600**:  
  - Lamp types: Deuterium (190–510 nm) and Tungsten Halogen (430–710 nm)  
  - Detection: 256 diode array with 1.25 nm dot pitch  
  - Bandwidth selectable: 4–25 nm  
  - 4 acquisition channels and 4 spectra stored  
  - Wavelength accuracy ≤1 nm, precision ≤0.5 nm  
  - Automatic wavelength validation with holmium oxide filter  
  - Noise (ASTM E1657-94) ≤1×10⁻⁵ AU (D2 lamp), drift ≤5×10⁻⁴ AU/h  
  - Measurement range up to 2.2 AU, time constants selectable 0.1 to 10 s  

- **Smartline Pump 1000**:  
  - Double-piston pump with main and auxiliary pistons, automatic backflushing  
  - Flow rate: 0.001–9.999 ml/min externally controlled  
  - Flow accuracy <0.5%, precision <0.1% at 1 ml/min and 12 MPa  
  - Residual pulsation <0.5% at 1 ml/min methanol:water (8:2)  
  - Max operating pressure 40 MPa  
  - Expandable with Smartline Manager 5000 to low pressure gradient system (up to 4 eluents)  
  - Option for high pressure gradient system (HPG)  

- **Spark Triathlon 900 Autosampler**:  
  - Injection reproducibility: full loop RSD ≤0.3%, partial loop RSD ≤0.5%, µl pick-up RSD ≤1.0%  
  - Sample capacity: 96 vials (1.5 ml), vial height 32–47 mm inclusive cap  
  - Injection volume range: 1 µl to 1 ml, increments of 1 µl  
  - Programmable needle wash and wash solvent bottle (250 ml)  
  - Built-in vial sensor and compressor for headspace pressure  
  - Fast sample access (<10 seconds), fast injection valve switching (<100 ms)  
  - Programmable priority sample option  

- **Jetstream 2 Plus Column Thermostat**:  
  - Provides precise temperature control for column stability (specific specs not detailed)  

This integrated system enables precise separation, detection, and sample handling with advanced degassing, flow accuracy, and UV detection capabilities suitable for routine and advanced HPLC applications.

Knauer Smartline 5000 HPLC System with UV Detector 2600, Pump 1000, Jetstream 2 Plus Thermostat, and Spark Triathlon 900 Autosampler

Advanced HPLC pump for efficient mg to gram sample purification with intuitive control and adaptability.

The Knauer Azura P2.1L is a preparative HPLC pump designed to handle a wide range of flow rates and pressure capabilities. It is expertly crafted for the purification of milligram and gram samples. Featuring Mobile Control, it offers intuitive standalone operation and seamless automation.

One of the highlights is the integrated automatic recognition of the pump head with RFID technology, ensuring quick adaptability for various applications. This preparative pump supports a broad flow range from 0.1 to 1,000 ml/min and accommodates different liquid chromatography software platforms. It includes improved electronic drive compensation, compressibility compensation, and leak management. The constant pressure operation mode ensures consistent performance.

Specifications:
- **Pump Type**: Preparative HPLC pump  
- **Delivery System**: Double piston pump with Al²O³ pistons  
- **Pump Head**: 250 ml/min, with titanium materials  
- **Pressure Sensor Materials**: PEEK  
- **Max Pressure**: 22.5 MPa (225 bar, 3260 psi) up to 100 ml/min  
- **Flow Rate Range**: 0.1-250 ml/min, recommended 0.5-200 ml/min  
- **Flow Rate Accuracy**: ± 2% using ethanol/water 10:90  
- **Flow Rate Precision**: < 0.2% RSD  

Gradient support includes isocratic, and it can be upgraded to a binary HPG or ternary LPG system. The system includes advanced features like soft start, programmable minimum and maximum pressure, and automatic detection of the pump head via RFID. Communication options include LAN, RS-232, and analog control. The device operates within a temperature range of 10–40 °C and features a leak sensor for security.  

With a robust design featuring titanium, stainless steel, and PEEK materials, this pump delivers durability and efficiency for demanding laboratory environments. The Azura P2.1L supports analytical flexibility and rapid adaptation for high precision chromatography needs.

Knauer Azura P2.1L Preparative HPLC Pump

Comprehensive HPLC system with intelligent components for precise chemical analysis.

The Jasco 1500 HPLC System is a sophisticated analytical tool designed for high-performance liquid chromatography applications. The system includes the following components: 

- **Jasco UV-1570M Intelligent UV Detector**: Delivers reliable and precise detection with intelligent features for data acquisition and management.
  
- **Jasco LG-1580-02 Ternary Gradient Unit**: Offers versatile and accurate gradient formation for complex separations.
  
- **Jasco PU-1580 Intelligent HPLC Pump**: Ensures stable flow rates and high precision in solvent delivery for consistent analysis results.
  
- **Jasco AS-1555 Intelligent Sampler**: Facilitates automated sample introduction, enhancing throughput and reducing human error.
  
- **2 Alltech Elite Degassing System**: Ensures bubble-free operation, enhancing chromatographic performance and reliability.
  
- **PC**: Integrates with the system for comprehensive control and data processing.

The Jasco 1500 HPLC System is ideal for laboratories requiring high precision and dependability in chromatographic analyses.

Jasco 1500 HPLC System: Advanced Analytical Solution

Laboratory refrigerator with 46 L capacity, temperature range 0-10°C, compact 510x430x480 mm size.

- **Temperature Range**: 0°C to 10°C  
- **External Dimensions (HxWxD)**: 510 x 430 x 480 mm  
- **Internal Dimensions (HxWxD)**: 425 x 360 x 390 mm  
- **Capacity**: 46 liters  
- **Weight**: 16.5 kg

Gastro-Cool GCKW50 Laboratory Refrigerator

An integrated solution for streamlined HPLC separation processes.

The Waters 2695 Separations Module offers an integrated platform for solvent and sample management, efficiently combining two essential HPLC functions. This integration enhances the overall workflow by streamlining all critical separation processes, providing reliable and precise results in high-performance liquid chromatography applications.

Waters 2695 HPLC Separation Module

Comprehensive HPLC system with advanced liquid handling and sample processing.

The Gilson GX-281 HPLC System is a comprehensive solution for high-performance liquid chromatography. This system integrates a Gilson GX-281 sample robot for precise automation, along with two Gilson 6 position valves ensuring efficient fluid management. Equipped with two Gilson 306 pumps and an additional Gilson 322 pump, this system offers reliable and versatile liquid handling capabilities, making it ideal for sophisticated chromatographic applications.

Gilson GX-281 HPLC System

Comprehensive HPLC system ideal for diverse analytical needs.

The Agilent Series 1100 HPLC System is a robust and versatile high-performance liquid chromatography solution. It includes the Agilent G1322A Degasser for efficient solvent degassing, the G1311A Quaternary Pump for precise solvent delivery, and the G1313A Autosampler for automated sample injection. Additionally, the system features the G1316A Thermostatted Column Compartment to maintain optimal column temperature, and the G1314A Variable Wavelength Detector for accurate detection across a wide range of wavelengths. This complete system is designed to enhance the accuracy and efficiency of your chromatographic analysis.

Agilent Series 1100 HPLC System

Efficient HPLC pump paired with precise autosampler for analytical precision.

The Thermo Scientific P4000 HPLC Pump combined with the AS3000 AutoSampler provides a highly efficient and precise solution for HPLC applications. The P4000 pump ensures consistent flow rates, while the AS3000 offers automated sampling accuracy, both essential for high-quality chromatographic performance.

TSP P4000 HPLC Pump and AS3000 AutoSampler

Multi-technique GC autosampler with syringe-only concept, up to 500µl injection, for headspace, SPME, LVI methods.

- **Application range**: Environmental, Food/Beverages/Consumer Products, Forensics, Chemicals/Polymers, Pharmaceuticals  
- **Key features**:  
  - Combines Static Headspace, SPME, In-Tube Extraction, and Liquid Injection in a single instrument  
  - Liquid injection volume capacity up to 500 µl, suitable for Large Volume Injection (LVI)  
  - Syringe-only concept: no sample loops, transfer lines, or valves in the sample path  
  - Capable of injecting into 4 different injectors without hardware modifications  
  - Top-mounted design conserves bench space  
  - High precision and increased sample capacity  
  - Compatible with major GC and GC-MS systems  
- **Technical specifications**:  
  - System type: XYZ robot with syringe-only concept (no tubing in sample path)  
  - Local user interface: Control panel with 4 function keys, graphical LCD display, unique scroll knob for teach functions  
  - Remote control software: Cycle Composer (compatible with Windows 98/NT4/2000/XP)  
  - Available GC mounting kits for:  
    - Agilent 5890 / 6850 / 6890  
    - ThermoFinnigan Trace 2000 / GC 8000 top  
    - Varian GC 3400 / 3600 / 3800 / 3900  
    - Shimadzu GC 14 / 17 / 2010  
    - Perkin Elmer Autosystem XL / Clarus 500

CTC Analytics Combi PAL GC Autosampler for multi-technique sample introduction

Comprehensive liquid chromatography system with precision flow control and variable detection options.

The Agilent 1100 HPLC System offers an advanced solution for precise liquid chromatography analysis. It includes the Agilent 1100 Degasser G1379A, two Agilent 1100 Isocratic Pumps G1310A, a Variable Wavelength Detector G1314A, and a Fluorescence Detector G1321A. The package also comes with two solvent trays and a manual injector featuring a 1 ml sample loop, all conveniently operated via an included laptop.

**Agilent 1100 IsoPump**:  
- Hydraulic system features dual piston with a servo-controlled variable stroke drive, floating pistons, and an active inlet valve.  
- Adjustable flow range from 0.001 to 10 ml/min with precision advancements of 0.001 ml/min.  
- Flow precision: < 0.3% RSD, typically 0.15% at 1 ml/min.  
- Pressure capabilities range up to 40 MPa for 5 ml/min flow, or up to 20 MPa for 10 ml/min flow.  
- Offers user-selectable compressibility compensation, with a pH tolerance from 1.0 to 12.5.  
- Compact design with dimensions of 140 x 345 x 435 mm and weight of 11 kg.

**Agilent 1100 VWD**:  
- Utilizes a double-beam photometer with a deuterium lamp spectral range of 190–600 nm.  
- Precision capabilities include a short-term noise of ± 0.75 x 10-5 AU at 254 nm.  
- Wavelength accuracy is maintained at ± 1 nm, using self-calibration and verification filters.  
- Flow cell features a 14-µl volume with a 10-mm path length enduring pressures up to 40 bar.  
- Dimensions (HxWxD) measure 140 x 345 x 435 mm, weighing 11 kg.

**Agilent 1100 FLD**:  
- Offers advanced multi-signal fluorescence detection with rapid on-line scanning, using a xenon flash lamp.  
- Excitation ranging from 200 to 700 nm, with emission ranging from 280 to 900 nm, gathered swiftly at 28 ms per data point.  
- Ensures wavelength accuracy of ±3 nm and repeatability of ±0.2 nm.  
- Features an 8 µl flow cell, capable of supporting up to 20 bar pressure.  
- Measurements are compactly encompassed within 135 x 345 x 435 mm dimensions, with a weight of 11.5 kg.

Agilent 1100 HPLC System

Complete HPLC system with degasser, pump, autosampler, and wavelength detector.

The Agilent Series 1100 High-Performance Liquid Chromatography (HPLC) System is a comprehensive solution designed for advanced chemical analysis. Components included in the system are:

- **Solvent Tray**
- **Vacuum Degasser**: Features a 4-channel configuration with tubular plastic membranes and a vacuum pump. Offers a flow rate of 0-10 ml/min per channel, adaptable for different degassing requirements.
- **Binary Pump**: Provides precise flow control with setpoints ranging from 0.001 to 5.0 ml/min. The pump can handle pressures up to 40 MPa with user-selectable compressibility compensation for various mobile phases.
- **Autosampler**: Incorporates safety features, including leak detection and low voltages in maintenance areas. Offers an injection range of 0.01 to 8 µl with high precision.
- **Thermostatted Column Compartment**: Allows temperature settings ranging from 10°C below ambient up to 80°C, maintaining temperature stability at ±0.15°C.
- **Variable Wavelength Detector**: Double beam photometer with a wavelength range of 190 – 600 nm and high accuracy to ensure reliable results.

This system is engineered for precision and reliability, supporting a wide range of research and analytical applications in high-performance liquid chromatography.

Agilent 1100 HPLC System with Integrated Detector

High-efficiency gas chromatography column with included performance test report.

The Agilent Technologies J&W DB-624 GC column excels in analytical performance, designed for precise separation in gas chromatography applications. It is accompanied by a test report that confirms its reliability and efficiency in analytical tasks, ensuring accurate and reproducible results in your laboratory processes.

GC Column with Test Report

Compact gas chromatograph for qualitative and quantitative analysis.

The Interscience Focus GC is a compact, one-channel gas chromatograph designed for both qualitative and quantitative analysis of mixture compositions. This sophisticated instrument is ideal for applications in toxicology, doping research, forensics, the petrochemical industry, food safety monitoring, and the pharmaceutical sector. It is equipped with an FID (Flame Ionization Detector) and a Thermo TriPlus autosampler with a reagent heater, ensuring precise and efficient analysis across various fields.

Interscience Focus GC Gas Chromatograph

Versatile FPLC system for precise protein and nucleic acid separations.

The GE Healthcare ÄKTApurifier UPC10 System is a highly versatile and modular liquid chromatography system designed for the fast and reliable separation of proteins, peptides, and nucleic acids through FPLC methods. It's engineered to purify microgram to milligram quantities of proteins with operational flow rates ranging from 0.001 to 10 ml/min under pressures up to 25 MPa. Its flow rate range is adaptable: from 0.01 to 100 ml/min in isocratic and gradient modes, with a doubled range in double mode up to 200 ml/min. It handles pressure up to 10 MPa accurately, ensuring pH stability from 1 to 13, or 1 to 14 with limited exposure. The system features a high degree of accuracy and reproducibility in flow rate and gradient composition. With a total volume of less than 800 µl/module, the wavelength range between 190–700 nm can be utilized in 1 nm steps. The system's comprehensive components include a UPC-900 monitor, P-900 and P-960 pumps, valves, and a fraction collector, making it an ideal solution for laboratory professionals.

GE Healthcare ÄKTApurifier UPC10 System

Advanced HPLC system with automated sample processing and precise detection capabilities.

The Gilson GX-281 HPLC System is an advanced laboratory solution designed for automated high-performance liquid chromatography. The system comprises a Gilson GX-281 sample robot, which ensures efficient sample processing. It features two 6-position valves and is equipped with both a Gilson 306 pump and a Gilson 321 pump for accurate liquid handling. Detection is achieved through a Gilson 159 UV-Vis detector, ensuring precise analysis results. The system is supported by robust software for enhanced control and data management.

Compact system for automated protein purification with real-time monitoring.

ÄKTA start is a compact, single-step purification system designed for the efficient and reliable purification of proteins. This modern, user-friendly system automates the protein purification workflow, offering features such as automated sample injection, fraction collection, and real-time monitoring. Method templates support common chromatography techniques, including Affinity Chromatography, Ion Exchange Chromatography, Gel Filtration, and De-salting. Quick start methods facilitate the purification of various common proteins, while predefined system methods clean the flow path. Operated via a touch screen on the instrument, the ÄKTA start system can also be controlled from a computer using the UNICORN start software. It includes a dedicated Fraction collector, Frac30, which accommodates fractions in four different tube sizes. 

Specifications:  
- Buffer selection: 2 buffer inlets  
- Flow rate: 0.5 – 5 ml/min  
- Operating pressure max: 0.5 MPa  
- Dimensions (DxHxW): 280 x 360 x 340 mm  
- Weight: 9 kg

GE Healthcare Äkta Start FPLC System

Comprehensive FPLC system for precise separation and analysis needs.

The General Electric Äkta Purifier FPLC System is a versatile and advanced setup designed for precise purification tasks in laboratory environments. This high-performance system includes a pH/C-900 module for accurate pH and conductivity detection, and a UV-900 multi-wavelength UV-Vis detector for comprehensive spectral analysis. The P-900 pump module ensures consistent and reliable fluid management, while the Frac-950 fraction collector, equipped with dual trays, efficiently gathers separated fractions, enhancing throughput and efficiency in chromatographic applications.

General Electric Äkta Purifier FPLC System

Portable and user-friendly device for precise INR monitoring of warfarin therapy.

The CoaguChek XS system is a convenient, portable and user-friendly instrument for monitoring warfarin therapy. It determines the INR value (International Normalized Ratio) from a drop of capillary whole blood – simple, precise and reliable. Accurate PT/INR results in one minute Built-in quality control checks every strip automatically Lab-comparable accuracy Detection system: Amperometric (electrochemical) determination of the PT time after activation of the coagulation with human recombinant thromboplastin User interface: Icon-based LCD display; on/off, mem and set buttons Measuring range: INR: 0.8 – 8.0, %Quick: 120 – 5, Seconds: 9.6 – 96

Chromatography

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