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GE healthcare Streamline 25 Column
Expanded bed adsorption (EBA) is a single pass operation to recover target molecules such as proteins from crude stock feedstock without prior clarification. In extracellular processes, EBA is effective in capturing molecules from fermentation or cell culture. In intercellular processes with soluble molecules, it is suitable for use with broth from cell lysis or homogenization.
Streamline columns are specially designed for use with Streamline adsorbents. Column design incorporates spacial flow distributors and a moveable adaptor. The distributor at the bottom of the column creates the stable fluidized bed required for the technique. The moveable adaptor allows the bed height to be adjusted for elution in sedimented bed mode.
Diameter: 25 mm
Column cross section: 491 mm2
Tube height: 1000 mm
Stand foot print: 430 x 430 mm
Sedimented bed height: 150 – 300 mm
Sedimented adsorbent volume: 75 – 150 mL
Max pressure: 1 bar
Operating temperature: 4°C to 40°C
Feed application: 200 – 400 cm/h
Elution sedimented bed: 50 – 150 cm/h
Elution expanded bed: 100 – 300 cm/h
Chemicals used with Streamline 100 column:
Acetic acid: Concentration – 25%, pH = 1.8; Time/cycle – 3 h
Acetone: Concentration – 2%; Time/cycle – 15 min
Ammonium sulphate: Concentration – 2 M; Time/cycle – 5 h
Ethanol: Concentration – 20%; Time/cycle – 24 months
Ethanol: Concentration – 70%; Time/cycle – 1 h
Ethanol/Acetic acid: Concentration – 20%/10%; Time/cycle – 3 h
Ethylene glycol: Concentration – 50%; Time/cycle – 3h
Guanidine-Hydrochloric acid: Concentration – 6 M; Time/cycle – 5 h
Glycerol: Concentration – 10%; Time/cycle – 3 h
Hydrocloric acid: Concentration – 0.1 M, pH = 1; Time/cycle – 1 h
Isopropanol: Concentration – 30%; Time/cycle – 3 h
Sodium acetate/Ethanol: Concentration – 0.2 M/20%; Time/cycle – 24 h
Sodium chloride: Concentration – 0-3 M; Time/cycle – 3 h
Sodium hydroxide: Concentration – 2 M, pH = 14; Time/cycle – 24 h
Sodium hydroxide: Concentration – 0.01 M, pH = 12; Time/cycle – 12 months
Sodium sulphate: Concentration – 1 M; Time/cycle – 3 h
Triton X 100: Concentration – 1%; Time/cycle – 5 h
Tween: Concentration – 1%; Time/cycle – 5 h
Urea: Concentration – 8 M; Time/cycle – 5 h
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