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Shimadzu MCE-202 MultiNA Microchip Electrophoresis System
This system is used to analyze the size of DNA/RNA samples, with convenientanalytical operability. It achieves analysis costs on par with agarose gel electrophoresis, and can perform fully automatic analyses of up to 108 samples. Using optimized reagent kits (four types for DNA analysis and one type for RNA analysis), the system achieves a high resolution and high sensitivity. It can significantly improve the workflow for mutation checks in genome editing, and genotype determination.
Sample rack: Compatible with 96-well PCR plate and 12/8-strip PCR tube (Shimadzu recommended product)
Microchip: Quartz, 23 mm separation channel length, on-chip electrodes (insert up to four microchips)
Pretreatment: Automatic sample injection, automatic separation buffer replenishing, automatic chip cleaning
Electrophoresis Voltage: Max. rated voltage: 1.5 kV, max. current: 250 μA
Analysis Cycle time: Approx. 80 s (using four chips) DNA standard analysis (DNA-1000/premixed). This does not include time required for carrying out the first and final wash and initial analysis.
Detection Method: LED-excited fluorescence detector (470 nm excitation wavelength)
Separation Size Range: 25 to 500 bp (DNA-500 Kit), 100 to 1000 bp (DNA-1000 Kit), 100 to 2500 bp (DNA-2500 Kit), 100 to 12000 bp (DNA-12000 Kit), Up to 28S rRNA (5.0 knt) (RNA Kit)
Resolution: 5 bp (25 to100 bp), 5% (100 to 500 bp), 10% (500 to 1000 bp), 20% (1000 to 12000 bp)
Sizing Accuracy: ±5 bp (25 to 100 bp), ±5% (100 to 500 bp), ±15% (DNA-1000, DNA-2500, DNA-12000)
Required Sample Volume: DNA analysis: Premix mode: 2 to 10 μL (after mixing with marker solution: 6 to 30 μL), On-Chip Mixing mode: 5 to 30 μL, RNA analysis: Premix mode: 3 to 15 μL (after mixing with marker solution: 6 to 30 μL), In the Premix mode, the marker solution is mixed with the sample before loading in the instrument, In the On-Chip Mixing mode, the sample and marker solution are loaded separately and mixed on the microchip under program control.
Maximum Salt Concentration: DNA analysis: 10mM Tris-HCI containing 125 mM KCl or NaCl max, RNA analysis: 10mM Tris-HCI, containing 1 mM EDTA max.
Min. Detection Limit: DNA analysis: 0.2 ng/μL (at 10mM Tris-HCI buffer, containing 50 mM KCl and 1.5 mM MgCl2), RNA analysis: 5 ng/μL (total RNA), 25 ng/μL (mRNA) (at 10 mM Tris-HCI buffer, containing 1mM EDTA)
Quantitation Range: DNA analysis: 0.5 to 50 ng/μL (at 10mM Tris-HCI, containing 50 mM KCl and 1.5 mM MgCl2), RNA analysis: 25 to 500 ng/μL (total RNA), 25 to 250 ng/μL (mRNA) (10 mM Tris-HCI buffer, containing 1 mM EDTA)
Quantitation Accuracy: DNA analysis: ±30% (at 10 mM Tris-HCI buffer, containing 50 mM KCL) (DNA-500, DNA-1000, and DNA-2500 Kits), ±40 % (DNA-12000 kit. Quantitative accuracy is based on verification from 200 bp to 12000 bp.)
Quantitation Repeatability: RNA analysis: CV 10% or less (CV 20% or less for eukaryotic-origin total RNA at concentrations of 150 ng/μL or more)
Dimensions (WxDxH): 415 x 545 x 508 mm
Weight: 43 kg
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