Electrophoresis

The redesigned wide Mini-Sub Cell GT Electrophoresis Cell offers updated features that make electrop (more)horesis even easier. The wide Mini-Sub Cell GT Cell is suited for multiple-sample, rapid-screening applications. This popular system has a wide platform that can separate 30 samples per comb. The wide Mini-Sub Cell GT Cell is the same width as the Sub-Cell GT Cell, so the comb holder, combs, and 15 x 10 cm gel trays are interchangeable with the larger Sub-Cell GT units. All wide Mini-Sub Cell GT Systems accommodate ReadyAgarose Precast Gels to save time and allow highly reproducible separations. The cells include a buffer tank, a safety lid with cables, and a leveling bubble. Cell size (WxLxH): 178 x 255 x 68 mm Gel tray sizes (WxL): 150 x 70 mm ReadyAgarose gels accommodated: Yes, wide mini and 96 Plus formats Sample throughput: 10 – 60 Base buffer volume: ~650 ml Buffer recirculation: No Bromophenol blue migration: ~4.5 cm/hr (at 75 V)

System consists of: Bio-Rad Mini-Sub Cell GT Electrophoresis Cell Bio-Rad PowerPac HV High-Voltage P (more)ower Supply Mini-Sub Cell GT: Bio-Rad offers a range of mini horizontal electrophoresis chambers to resolve DNA fragments on either handcast agarose gels or ReadyAgarose precast gels. These cells include a buffer tank, safety lid with cables, and leveling bubble. Fast and flexible, the Mini-Sub cell GT cell is our most popular classroom gel box. The Mini-Sub cell GT cell holds either a 7 x 7 cm or a 7 x 10 cm gel tray. A 10 cm agarose gel poured with two 8-well combs easily accommodates two student teams at a time. At 100 V, this cell resolves DNA fragments from 20–20,000 base pairs in just under 30 min. Cell size (WxLxH): 120 x 260 x 65 mm Gel tray sizes (OD) (WxL): 70 x 70 mm ReadyAgarose gels accommodated: Yes, mini format Sample throughput: 8–30 Base buffer volume: ~270 ml Buffer recirculation: No Bromophenol blue migration: 4.5 cm/hr (at 75 V) PowerPac HV: Output (programmable): Voltage 20–5,000 V, Current 0.01–500 mA, Power 1–400 W Type of output: Constant voltage, constant current, constant power, or constant temperature (with automatic crossover) Timer control: 1 min–99 hr 59 min, fully adjustable Volt-hour control: Yes, 99,999 V-hr Pause/resume function: Yes Display functions: 128 x 64 pixel, yellow-green backlit graphics LCD Programmable methods: Stores up to 9 basic and 9 IEF methods, each with up to 9 steps Real-time editing: Yes Real-time clock: Yes Automatic recovery after power failure: Yes, user-selectable; setup values maintained Data transfer/archiving: Yes Temperature control: Yes, via temperature probe (not included); 30–90°C ±2°C Microampere readout and control: Yes Safety features: No-load detection, sudden load change detection, ground leak detection, overload/short circuit protection, overvoltage protection Number of output jacks: 4 sets in parallel Dimensions (WxLxH): 75 x 340 x 100 mm Weight: 2.85 kg

The QIAxcel Advanced system replaces traditional, labor-intensive gel analysis of DNA and RNA by ful (more)ly automating sensitive, high-resolution capillary electrophoresis of up to 96 samples per run. The QIAxcel ScreenGel software ensures convenient analysis and documentation of data. The QIAxcel Advanced system operates in conjunction with the QIAxcel DNA Kits and QIAxcel RNA QC Kit v2.0

Power Supply power-phor-N Power Supply for Electrophoresis, 220 – 260 V, 10 – 500 V Output Range Vol (more)tage, 10 – 1000 mA Output Range Current, 200 W max. Output Range Wattage

LM2536 Mini Electroforesis Cell

High voltage power supply with constant voltage, constant current or constant power regulation mode.

The nanoPAC Mini Power supplies are an ultra compact and economic unit ideal for use with DNA (Horiz (more)ontal) and Protein (Vertical) electrophoresis systems. With enhanced features, such as a maximum constant voltage up to 300 and maximum constant current output of 400mA. The nanoPAC’s user- friendly interface is easily adjustable in 1V and 1mA increments, making it perfect for separations where precise settings are required. Its ultra compact size and two pairs of parallel power terminals, which can run two electrophoresis units simultaneously, save time and bench space. Maximum Voltage: 300 V Maximum Current: 400 mA Maximum Power: 60 W Number of Output Pairs: 2

The Horizon 20-25 Horizontal Gel Electrophoresis Apparatus is designed for separation of preparative (more) and analytical quantities of nucleic acids. It is suitable for agarose gel electrophoresis procedures in which buffer circulation may be required. The Horizon 20-25 apparatus is designed for simplified gel casting and electrophoresis. This apparatus is engineered for durable performance and easy storage. Current range: 4 – 360 mA, 0.5 A max Voltage range: 250 VDC Max

Cell size (WxLxH): 92 x 255 x 56 mm Gel tray sizes (WxL): 70 x 70 mm Sample throughput: 8-30 Base bu (more)ffer volume: ~270 ml Bromophenol blue migration: ~4.5 cm/hr (at 75 V)

Voltage range: 0 – 500 V Amperage range: 0 – 500 mA Power range: 0 – 150 W Timer: 1 – 999 min/0.1 –  (more)99.9 h Outputs dual in parallel, floating Leakage detection: > 500 μA No load detection: < 0.1 W Dimensions: 300 x 120 x 180 mm Weight: 5 kg

The Criterion cell is a midi electrophoresis cell dedicated to running one or two Criterion gels, wh (more)ich are wider and longer than traditional mini gels. With a single Criterion gel, you can run up to 26 samples in less than 1 hr or accommodate an 11 cm ReadyStrip IPG strips for 2-D applications. The Criterion cell accommodates Criterion precast or handcast gels. Features and Benefits: Compact size requires only 1 L running buffer Integrated cassette buffer chamber simplifies assembly and ensures that the system will never leak Safe, easy-to-open cassettes use a built-in wedge on the lid to open gel cassettes in a single step Locator slots built into the tank walls allow easily and quickly slide cassettes into position Ability to separate more samples in fewer runs and resolve more proteins Run 2-D gels in less than a day Applications and Uses: Polyacrylamide gel electrophoresis (PAGE or SDS-PAGE) 2-D gel electrophoresis Screen new samples Evaluate sample preparation conditions Number of gels: 1–2 Precast gels: Criterion precast gels Handcast gels: Gels prepared in Criterion empty cassettes Gel size (WxL): 133 x 87 mm Gel thickness: 1.0 mm Total buffer volume: 1 L Typical running conditions: 200 V constant Recommended power supply: PowerPac Basic or PowerPac HC Dimensions (WxLxH): 144 x 223 x 195 mm Weight: 0.86 kg

The power source of the brand Pharmacia type MultiDrive XL is designed for use in combination with e (more)lectrophoresis and isoelectric focusing (IEF). This power source can control and supply the three electrical parameters voltage, current and power. The duration of the run can be controlled by setting values for the following parameters: time, voltage and milliamperes per hour. In addition, the Pharmacia MultiDrive XL contains nine pre-programmed methods. Specifications Voltage: 3500V Current: 400mA Power: 150W Dimensions (WxDxH): 365 x 300 x 160 mm

Bio-Rad offers a range of mini horizontal electrophoresis chambers to resolve DNA fragments on eithe (more)r handcast agarose gels or ReadyAgarose precast gels. These cells include a buffer tank, safety lid with cables, and leveling bubble. Fast and flexible, the Mini-Sub cell GT cell is our most popular classroom gel box. The Mini-Sub cell GT cell holds either a 7 x 7 cm or a 7 x 10 cm gel tray. A 10 cm agarose gel poured with two 8-well combs easily accommodates two student teams at a time. At 100 V, this cell resolves DNA fragments from 20–20,000 base pairs in just under 30 min. Cell size (WxLxH): 120 x 260 x 65 mm Gel tray sizes (OD) (WxL): 70 x 70 mm ReadyAgarose gels accommodated: Yes, mini format Sample throughput: 8–30 Base buffer volume: ~270 ml Buffer recirculation: No Bromophenol blue migration: 4.5 cm/hr (at 75 V)

This system is used to analyze the size of DNA/RNA samples, with convenientanalytical operability. I (more)t achieves analysis costs on par with agarose gel electrophoresis, and can perform fully automatic analyses of up to 108 samples. Using optimized reagent kits (four types for DNA analysis and one type for RNA analysis), the system achieves a high resolution and high sensitivity. It can significantly improve the workflow for mutation checks in genome editing, and genotype determination. Sample rack: Compatible with 96-well PCR plate and 12/8-strip PCR tube (Shimadzu recommended product) Microchip: Quartz, 23 mm separation channel length, on-chip electrodes (insert up to four microchips) Pretreatment: Automatic sample injection, automatic separation buffer replenishing, automatic chip cleaning Electrophoresis Voltage: Max. rated voltage: 1.5 kV, max. current: 250 μA Analysis Cycle time: Approx. 80 s (using four chips) DNA standard analysis (DNA-1000/premixed). This does not include time required for carrying out the first and final wash and initial analysis. Detection Method: LED-excited fluorescence detector (470 nm excitation wavelength) Separation Size Range: 25 to 500 bp (DNA-500 Kit), 100 to 1000 bp (DNA-1000 Kit), 100 to 2500 bp (DNA-2500 Kit), 100 to 12000 bp (DNA-12000 Kit), Up to 28S rRNA (5.0 knt) (RNA Kit) Resolution: 5 bp (25 to100 bp), 5% (100 to 500 bp), 10% (500 to 1000 bp), 20% (1000 to 12000 bp) Sizing Accuracy: ±5 bp (25 to 100 bp), ±5% (100 to 500 bp), ±15% (DNA-1000, DNA-2500, DNA-12000) Required Sample Volume: DNA analysis: Premix mode: 2 to 10 μL (after mixing with marker solution: 6 to 30 μL), On-Chip Mixing mode: 5 to 30 μL, RNA analysis: Premix mode: 3 to 15 μL (after mixing with marker solution: 6 to 30 μL), In the Premix mode, the marker solution is mixed with the sample before loading in the instrument, In the On-Chip Mixing mode, the sample and marker solution are loaded separately and mixed on the microchip under program control. Maximum Salt Concentration: DNA analysis: 10mM Tris-HCI containing 125 mM KCl or NaCl max, RNA analysis: 10mM Tris-HCI, containing 1 mM EDTA max. Min. Detection Limit: DNA analysis: 0.2 ng/μL (at 10mM Tris-HCI buffer, containing 50 mM KCl and 1.5 mM MgCl2), RNA analysis: 5 ng/μL (total RNA), 25 ng/μL (mRNA) (at 10 mM Tris-HCI buffer, containing 1mM EDTA) Quantitation Range: DNA analysis: 0.5 to 50 ng/μL (at 10mM Tris-HCI, containing 50 mM KCl and 1.5 mM MgCl2), RNA analysis: 25 to 500 ng/μL (total RNA), 25 to 250 ng/μL (mRNA) (10 mM Tris-HCI buffer, containing 1 mM EDTA) Quantitation Accuracy: DNA analysis: ±30% (at 10 mM Tris-HCI buffer, containing 50 mM KCL) (DNA-500, DNA-1000, and DNA-2500 Kits), ±40 % (DNA-12000 kit. Quantitative accuracy is based on verification from 200 bp to 12000 bp.) Quantitation Repeatability: RNA analysis: CV 10% or less (CV 20% or less for eukaryotic-origin total RNA at concentrations of 150 ng/μL or more) Dimensions (WxDxH): 415 x 545 x 508 mm Weight: 43 kg