Agilent NovoCyte Penteon Flow Cytometer with 5 Lasers, 30 Colors, 32 Detectors, NovoSampler Q & Fluidics Station II

The Agilent NovoCyte Penteon is a high-performance multiparameter flow cytometer engineered for advanced cell analysis and complex assay workflows in biotechnology, molecular diagnostics, and immunophenotyping research. Featuring 5 solid-state lasers (UV 349 nm, Violet 405 nm, Blue 488 nm, Yellow 561 nm, Red 637 nm) with onboard thermal-electric cooling, it delivers up to 30 fluorescent colors across 32 individual detectors, enabling detailed phenotyping and functional studies critical in synthetic biology and biopharmaceutical development.

Included is the NovoSampler Q, an automated sample handler compatible with 12×75 mm tubes and various microplate formats up to 384 wells, facilitating high-throughput screening and robust plate-based analyses in gene editing and cell engineering workflows. The Fluidics Station II ensures precise, stable flow rates (5 to 120 μL/min) and volumetric absolute count precision (<5%), minimizing carryover (<0.1%) for reproducible quantification essential in molecular cloning and bioprocessing pipelines.

Optical detection employs high-efficiency silicon photomultipliers (SiPM) for sensitive fluorescence measurement with thresholds as low as FITC ≤40 MESF, PE ≤10 MESF, and APC ≤10 MESF, supporting rare event detection in clinical diagnostics and live-cell imaging. Forward scatter (FSC) and side scatter (SSC) sensitivity reach 0.4 μm and 0.1 μm, respectively, enhancing particle sizing and granularity resolution.

The system ships with a one-year subscription to De Novo FCS Express 7 Analysis Software (RUO version), providing an intuitive interface, advanced gating and kinetic tools, and comprehensive visualization options including histograms and heat maps. The software supports multiple cytometry file formats, ensuring seamless data integration for detailed statistical analysis and automated reporting, widely adopted in synthetic biology research and immunophenotyping.

This flow cytometer is validated through stringent QC including bead calibration, laser power stability, fluidics priming, and automated performance tracking with reproducible coefficient of variation (CV) metrics. Compact and modular, it supports complex immunoassays, high-content immune profiling, and automated plate-based screening, making it a robust choice for biotechnology labs focusing on molecular diagnostics, biomanufacturing, and biomedical engineering.

Key features include:

The Agilent NovoCyte Penteon is essential for researchers requiring precise, multiparameter flow cytometry in molecular biology, clinical diagnostics, and bioprocessing environments, supporting workflows such as live-cell imaging, gene editing phenotyping, and synthetic biology applications.