Microplate equipment

Manifold Type: 96-well washing, 8-channel dispense/aspiration (1 x 8) Washing Speed: ≤130 seconds (3 (more)-cycle wash, 300 μL/well, 1 x 8) Wash Cycles: 1 – 10 Volume Range: 25 – 3000 μL/well Dispense Precision: ≤ 3% CV Residual Volume: ≤ 2 μL/well Soak Time: 1 – 600 seconds Shaking: User-programmable speeds and timing Dimensions (DxWxH): 406 x 356 x 165 mm Weight: 9.8 kg

2x Plate washer uWasher DG-34 Grifols (One in good condition, 1 for parts)

Microplate type: 96-well Onboard Software: Create, edit and run multiple protocols Soaking: Programm (more)able up to 60 minutes Volume range: 50 to 3000 microliter increments Wash cycles: 1 – 10 Dispense precision: ≤3% CV: 300 µL/well Residual volume: <2 µL/well Wash speed: 96-wells, 300 µL/well, 3 cycles: <30 seconds Vacuum filtration: 5 to 999 seconds Including vacuum pump

The SkanWasher 300 is an automatic 96-well microplate washer designed to give reliable, reproducible (more) results, with no cross-contamination between wells, and no carryover from plate to plate. The washer incorporates flexible software, and can handle most requirements for kit manufacturers, and research and clinical scientists. The SkanWasher 300 has eight user-defined programs, two inlets for buffer and rinse, and a special rinse program. The fluid pressure can be adjusted individually for each program, making it suitable for very gentle washing, as well as tougher, more turbulent washing. Washes all 96 wells simultaneously 8 user defined programs with 16 steps per program. Wash steps set by either time or volume (100 – 1000 ml) Versatile dispense-only function provides < 4% CV dispense accuracy and precision across the plate Easy to maintian: preset factory rinse program and removable wash head Wash head & manifold combined in one module, thereby reducing risk of contaminant growth Unique double probe washing system provides maximum washing efficiency with minimal reagent consumption Aspirate from top of liquids to avoid cross-contamination of wells Easily-accessible wash head channels for easy cleaning Motorized plate carriage, adjustable for flat & round bottomed plates Quick-disconnect fittings to avoid spills Capacity: 96-well Microplates Soak Time: Programmable to 3 Minutes Dimensions (WxDxH): 660 x 330 x 610 mm

The 2103 EnVision Multilabel Plate Reader is a fast, sensitive and versatile benchtop reader that de (more)livers optimized performance in every application and for every label. The unique design features modular label-specific optical mirror modules, high energy flash lamps, and high speed detectors. Light Source: Xenon flash lamp Plate Formats: 1 to 3456 wells Temperature range: from 2°C above ambient up to 45°C (0.5 °C increments) Uniformity: ± 1°C Heating time: < 10 minutes (from ambient up to 37°C) Shaking: linear, orbital, dual orbital Dispense volume: 2-475 µL (1 µL increments) Pump speed: 100-500 µL/s Dead volume: 750 µL. With pump-back function < 50 µL using stardard tubing length and factory settings. Typical Throughput: Fi/Abs (On the fly): 96 wells – 22 s, 384 wells – 27 s, 1536 wells – 36 s Fi/Abs (10 flash): 96 wells – 32 s, 384 wells – 1 min 03 s, 1536 wells – 2 min 56 s Fluorescence polarization (30 flash): 96 wells – 37 s, 384 wells – 1 min 22 s, 1536 wells – 4 min 15 s TRF LANCE (30 flash): 96 wells – 37 s, 384 wells – 1 min 22 s, 1536 wells – 4 min 15 s TRF DELFIA (100 flash): 96 wells – 55 s, 384 wells – 2 min 32 s, 1536 wells – 8 min 52 s AlphaScreen STD: 96 wells – 1 min 32 s, 384 wells – 4 min 54 s, 1536 wells – 19 min 28 s AlphaScreen HTS: 96 wells – 51 s, 384 wells – 1 min 52 s, 1536 wells – 9 min 23 s Detection Limit: Fluorometry (Fluorescein): 96 wells (200 µL) – < 4 pM/ < 0.8 fmol/well, 384 wells (50 µL) – < 4 pM/ < 0.2 fmol/well, 1536 wells (7.5 µL) – < 20 pM/ < 0.15 fmol/well Fluorescence polarization (Fluorescein 1 nM, SD): 96 wells- < 3 mP, 384 wells – < 2 mP, 1536 wells  – < 7 mP TR-Fluorometry (Europium): 96 wells (200 µL) – < 55 fM/ < 11 amol/well, 384 wells (50 µL) – < 55 fM/ < 3 amol/well, 1536 wells (6.6 µL) – < 150 fM/ < 1 amol/well Standard Luminometry (ATP): 96 wells (200 µL) – < 75 pM Enhanced Luminometry (ATP): 384 wells (50 µL) – < 10 pM Photometry Measuring range: 96 wells – 0 to 4 OD, 384 wells – 0 to 4 OD, 1536 wells – 0 to 3 OD Accuracy (at 2 OD): < 2 % Precision (at 2 OD): < 0.1% AlphaScreen (STD and HTS): 384 wells (25 µL, phosphorylated bio-peptide, kinase assay): < 100 amol Dimensions (HxDxW): 580 x 550 x 420 mm Weight: 50 kg

The ETI-MAX 3000 is a fully automated microtiter plate analyzer performing the complete sample proce (more)ssing (sample pre-dilutions, sample and reagent dispensing, incubations, wash processes, plate transports) as well as the photometric measurement and evaluation. The instrument is controlled by Windows PC software. This software allows the user to process the pre-defined assays of DiaSorin as well as assays programmed by the user. The clear structure with intuitive user-guidance allows simple and quick operation of daily routine jobs as well as programming of user-specific assays. Liquid handling: 1 ml thinner Disposable tips: 300 or 1100 µl Pipette range: Simultaneous operation of 4 plates Precision (samples and reagents): CV < 8.0% at 10 µl, CV < 2.5% at 100 µl Level sensor: Electronic Clot detection: Yes Mixing: Yes (pre-dilution tube and microplate) Multipipetting: Yes (sample, control and reagent) Pipetting time samples: < 18 min/96 wells (100 µl/well) Pipetting time reagents: < 4 min/96 wells (100 µl/well) Carry-over: None Identification: Barcode scanner for primary vessels, controls and reagents, Barcode scanner for microtiter plates Tube: Diameter 10-16 mm, Height 55-100 mm Barcode type: 2/5 interleaved, UPC A & E, 2/5 IATA, 2/5 Industrial, EAN 8 of 13, Code 128, EAN 128, Pharmacode, EAN addendum, 2 of 5, Code-a-bar Capacity: Maximum 240 sample tubes Capacity: 4 separate incubators Temperature range: 5°C above room temperature to 50°C Accuracy: ± 1°C average of the plate Consistency: ± 0.7°C/whole plate Shaking: Lengthwise Capacity: Up to 4 wash buffers Wash head: 1 x 8 Pipette volume: 200 – 2500 µl/well, depending on the assay Precision: ± 5% CV at 300 µl Residual volume: < 2.5 µl in round-bottom wells, < 4 µl for flat-bottom wells Level sensor buffer: Yes Level sensor waste container: Yes Wash programs: 1 to 9, depending on the assay Incubation time: 1 to 999 seconds, depending on the assay Pipette pressure: Adjustable for each assay individually Measurement: Vertical with photodiodes, Extinction or kinetic measurement Channels: 8 Operation: Single or double, with second measurement when the measuring range is exceeded Spectrum: 400 – 700 nm Filter: Up to 8 positions are available, of which 5 are already on board (405, 450, 492, 550, 620 nm) Measurement time: < 10 seconds Dynamic range: – 0.100 to 3,000 absorption units Linearity: 0 –2,000 absorption units ± 1.0% Precision: ± 0.005 absorption units or 2.5% Dimensions (WxDxH): 1130 x 760 x 1000 mm Weight: 130 kg

This system measures the growth of micro-organisms. This system features a unique incubator incorpor (more)ating a liquid circulation heat exchanger which provides constant temperatures to all wells, a linear shaker to assure good mixing in each well, a sensitive reader that measures turbidity, a technique more sensitive than a spectrophotometer. Additionally, the system is equipped with 8 filters to measure specific wavelengths, two covered honeycomb plates may be run at one time that makes it possible to run 200 samples simultaneously. Linear range: 0.0 – 2.0 A (max deviation <2%) Noise: max 0.002 A at 0.0 A Drift: max 0.003 A Resolution: 0.001 A Warm-up time: 30 min Light source: Quartz-Halogen lamp 8V/50W Filters: 405, 420, 450, 492, 540, 580, 600 nm and wide band filter 420 – 580 nm Half band with: 3 – 10 nm Detector: Silicon photocell Microplate type: Honeycomb 2 microplate, 100 wells per plate Capacity: 1 – 2 microplates Maximum volume: 400 µL/well Incubating temperature: 1.0 – 60.0°C, selectable with steps of 0.1°C. (Depends on the ambient temperatue. Bioscreen can go 6°C below or 30°C above ambient temperature up to a max of 60°C) Temperature uniformity: ± 0.1°C Heating time from 20°C to 30°C: 30 min Heat transfer fluid required: Mixture of ethylene glycol and water 1:1 Dimensions (WxDxH): 620 x 380 x 230 mm Weight: 22 kg

Tecan Genios Pro Multifunction Microplate Reader: The Tecan Genios Pro is a fully automatic, compute (more)r controlled Fluorescence. Luminescence and Absorbance instrument for measuring samples in a microplate. The instrument fulfills the requirements for numerous fluorescence, absorbance and luminescence applications in research and routine investigations. The filters are arranged in filter slides; four excitation filters and four emission filters respectively can be installed in the slides. Handles 6, 24, 96 and 384 well plates. Spectral range 360-700nm for fluorescence. 230-900nm absorbance spectral range. Sensitivity ≤1.5 picogram fluorescein on each well or ≤4 fmol well. The emission signal is measured by a highly sensitive PMT. Different detection techniques with one instrument change of measurement directions (top / bottom) by PC software. Thermal incubation at +5º C to + 40º C. Dual optical channels allow measurements from above or below the plate by mouse-click. Light to and from the samples is focused by a lens and high quality filters are easily accessible from the front of the instrument. Each filter slide can hold up to four filters. Orbital shaking and temperature control are standard. Plate formats: 6 to 384 well plates Absorbance Range: 230-999nm Temperature control: Ambient + 5 °C to 42 °C Shaking: Linear or orbital, 3 velocities Fluorescence Intensity Wavelength range: Excitation: 230 – 850 nm, emission: 280 – 850 nm Bandwidth: Adjustable from 2.5 nm – 10 nm below 280 nm, 5 nm – 20 nm above 280 nm Wavelength accuracy: ± 1 nm Wavelength precision: ± 1 nm Sensitivity (top reading – 384 well plate): 0.2 fmol/well Sensitivity (bottom reading – 384 well plate): 0.6 fmol/well Time Resolved Fluorescence Sensitivity (384 well plate): 60 amol/well Precision (384 well plate): 3 mP standard deviation Fluorescence Polarization Wavelength Range: 275-750nm Absorbance (96 well) Wavelength range: 230 – 999 nm Wavelength accuracy (492nm): ±/- 1% and ±/- 0.010 OD OD Wavelength precision (492nm): ±/- 10,5% and ±/- 0.005 OD OD Measurement range: 0 – 4 OD Glow Luminescence Type: Chemi or Bioluminescence Wavelength readings: 400-700nm

MicroFill is an economical, compact, and reliable dispenser for 24-, 96-, and 384-well microplates.  (more)An autoclavable fluid path is ideal for applications that require sterility. The dispense flow rates are easily selected to accommodate sensitive cell-based assays or vigorous dispensing for ELISA. Accurate and precise dispensing are guaranteed, without the time-consuming recalibration, cassette replacement, and maintenance commonly associated with other dispensers. Intuitive, on-board software is easy to use and saves time in instrument setup. Microplate Types: 24-, and 96-well, Low-profile, standard, and deep-well formats Manifold Type: One 8-tube (1 x 8) manifold−316 stainless steel tubes Dispense Speed: 96 wells, 10 µL/well, 1 x 8: 8 s Volume Range 5−6,000 µL/well, Minimum prime volume: 10 mL Flow Rates: User-programmable rates from high to low Dispense Accuracy: ± 1 µL at 5 µL, ± 1 µL at 20 µL, ± 1% at 100 µL Dispense Precision: ≤ 5% CV at 5 µL, ≤ 2.5% CV at 20 µL, ≤ 1% CV at 100 µL Dimensions (DxWxH): 460 x 380 x 180 mm Weight: 8.9 kg

Wavelength range excitation: 250 – 850 nm Wavelength range emission: 360 – 850 nm Wavelength bandwid (more)th: 9 nm Dynamic range: 6 decades Sensitivity top read: 3 fmol/well FITC Detector: Photomultiplier tube Read types: endpoint, kinetic, spectrum scan, well scan Wavelength selection: Monochromator tunable in 1-nm increments Light source: Xenon flash lamp Read time: 15 seconds Dimensions (HxWxD): 340 x 420 x 420 mm Excluding software As-is

Equipped with a StakMax microplate handler Washing Times 96 Wash Head, 300 µL/well: Single cycle; 11 (more) sec, Three cycles; 26 sec FastWash Times (optimized for speed), 300 µL/well: Three cycles; 14 sec Process Time With StakMax Microplate Handler (Fast Wash, 300 µL/well, three cycles): < 13 min for 20 plates Wash Volume: 100 – 3000 µL Residual Volume: ≤5 µL Dispense Volume: 10 – 400 µL per well Precision (at 300 µL): < 4% CV Shake Options: 3 speeds, Up to 600 sec max Wash Head: 96 wells, PET (polyethylene terephthalate), 304 stainless steel Liquid Contact Materials: Nylon, Polypropylene, Teflon, Polyethylene, Stainless Steel, and Silicone Dimensions washer (HxWxD): 210 x 466 x 517 mm Weight washer: 18.5 kg Dimensions StakMax (WxHxD): 440 x 231 x 509 mm Weight StakMax: 11.8 kg Total dimensions (WxD): 869 x 669 mm Total weight: 30.3 kg

Detection modes: Absorbance Read methods: End point, kinetic, linear scanning Microplate types: 96-w (more)ell plates Light source: Tungsten halogen Detector: photodiode Wavelength range: 380 to 900 nm Incl. Filters: 340, 405, 450, 490, 630 nm Dynamic range: 0 to 4.0 OD Resolution: 0.001 OD Pathlength correction: no OD accuracy: < 1% at 2.5 OD OD linearity: <1% at 2.5 OD, < 2% at 3.5 OD OD repeatability: < 0.5% at 2.5 OD, < 1.5% at 3.5 OD Reading speed (kinetic): 96 wells: 8 seconds Dimensions (WxDxH): 394 x 406 x 222 mm Weight: 13.6 kg Excl. software. Click for possible GEN5 basic/secure/IVD configurations

The SECTOR Imager offers high sensitivity and six logs of dynamic range. The SECTOR Imager 2400 read (more)s plates at a rate of approximately 3 ½ minutes per plate. Using MSD DISCOVERY WORKBENCH software, all models provide the capability to interact with a third party external robotic controller. The SECTOR Imager 2400 is compatible with: • 96- and 384-well MULTI-ARRAY plates • 96-well, 4-, 7-, and 10-spot MULTI-SPOT plates Signals generated by Free TAG, 15,000 [Cat # R94AA]: 12,000 – 18,000 counts Signals generated by PQ Low Control [Cat # R92TE-2]: 25 – 100 counts Dynamic Range: 106 Standard Deviation Dark Noise (depends on plate type): 0 – 16 counts Dimensions: 681 x 775 x 861 mm Weight: 64 kg Including PC

The HydroSpeed 384HT is Tecan’s advanced plate washer, designed for quick, parallel washing of entir (more)e 384-well plates. This avoids time differences between the wells for uniform results. In addition, the HydroSpeed provides full control of wash power for optimized washing of cells, beads and ELISAs. Extra gentle cell washing with tuneable wash power. Reliable operation through active prevention of needle clogging.

The BTX HT-200 plate handler allows for the Highthroughput System HT 25 or HT 96 for advanced molecu (more)lar delivery. The HT 96 (96-well) plates or the HT 25 (25-well) plates can be electroporated using a BTX generator (models: ECM 830 and ECM 630) with a plate handler. The Model HT-200 automatically switches columns in a Multi-well plate during electroporation using the Auto-track sensing featute. The Auto-track sensing allows rapid electroporation of an entire plate by setting up all columns and pressing one button. Voltage Range: 0 to 3000V DC Pulse Length Range: 10 µsec to 10 sec Pulse Number Range: 1 to 99 Dimensions (WxDxH): 230 x 215 x 140 mm Weight: 4.8 kg Including: 1x 96-Well Disposable Electroporation Plates, 4 mm, 250 µl, pkg. of 1 1x 96-Well Disposable Electroporation Plates, 2 mm, 125 µl, pkg. of 1 1x HT 25-Well Adapter for Plate Handler