Real-Time PCR

The offered instrument / system is fully functional tested and ready to use. FURTHER DETAILS TO FOLL (more)OW

The offered instrument / system is fully functional tested and ready to use. FURTHER DETAILS TO FOLL (more)OW

QIAGEN’s real-time PCR cycler, the Rotor-Gene Q, combines multiple optimized design features to prov (more)ide the outstanding performance and reliable results that your research demands. Together with optimized QIAGEN kits for real-time PCR, the Rotor-Gene Q enables streamlined analysis for a wide range of applications. Q-Rex Software is the new operating and analysis software for life science qPCR applications, with a plug-in concept that lets you add new functionality without affecting established workflows. A range of QIAGEN kits for the Rotor-Gene Q enables reliable quantification in all your real-time PCR applications without the need for optimization of reaction and cycling conditions. Kits for real-time PCR and HRM applications are available for: Gene expression analysis Pathogen detection DNA methylation analysis Genotyping and gene scanning miRNA research Channel: Blue Excitation: 365 ± 20 nm Detection: 460 ± 20 nm Examples of fluorophores detected: Marina Blue, Edans, Bothell Blue, Alexa Fluor 350, AMCA-X Channel: Green Excitation: 470 ± 10 nm Detection: 510 ± 5 nm Examples of fluorophores detected: FAM, SYBR Green I, Fluorescein, EvaGreen, Alexa Fluor 488 Channel: Yellow Excitation: 530 ± 5 nm Detection: 557 ± 5 nm Examples of fluorophores detected: JOE, VIC, HEX, TET, MAX, CAL Fluor Gold 540, Yakima Yellow Channel: Orange Excitation: 585 ± 5 nm Detection: 610 ± 5 nm Examples of fluorophores detected: ROX, CAL Fluor Red 610, Cy 3.5, Texas Red, Alexa Fluor 568 Channel: Red Excitation: 625 ± 5 nm Detection: 660 ± 10 nm Examples of fluorophores detected: Cy5, Quasar 670, LightCycler Red640, Alexa Fluor 633 Channel: Crimson Excitation: 680 ± 5 nm Detection: 712 high pass nm Examples of fluorophores detected: Quasar 705, LightCycler Red705, Alexa Fluor 680 Channel: HRM Excitation: 460 ± 20 nm Detection: 510 ± 5 nm Examples of fluorophores detected: SYBR Green I, SYTO9, LC Green, LC Green Plus+, EvaGreen Operating software is available. Analysis software must be purchased.

The Applied Biosystems 7500 Fast Real-Time PCR System offers maximum performance in the minimum time (more) for labs running a variety of applications, including High Resolution Melting (HRM) analysis. Fully optimized for Fast cycling, the 7500 Fast System delivers high-quality results in as little as 30 minutes. The 7500 Fast System enables more runs per day with the excellent performance equivalent to that offered by the 7500 System. The Applied Biosystems 7500 Fast System is the original Fast solution including validated Fast reagents and over 700,000 TaqMan Gene Expression Assays. Specially designed Fast block ensures thermal uniformity at top speeds TaqMan Fast Universal PCR Master Mix and Fast SYBR Green Master Mix enable-rapid results with excellent assay performance equivalent to that of standard real-time PCR Fast optical plates ensure excellent precision in 5 – 30 μL reaction volumes Fast ramp rates enable rapid results without compromising extension times or assay quality

LightCycler Nano Instrument – Real-time PCR amplification and detection instrument for tube-based as (more)says of up to 32 (4 x 8) samples per run, with minimal noise, footprint and weight. Assembled from just a few building blocks, the robust LightCycler Nano System is easy to transport and install. Up to 32 samples can be run in 0.2 ml tubes connected as strips. Fast heating and cooling is done using Peltier elements. Assay performance is supported further by a heated lid. Excitation is provided by high intensity LEDs filtered to provide light at 500 nm that is capable of exciting all fluorophores commonly used in qPCR. A prism is used to generate spectra from fluorescent emissions. These spectra are imaged using a CMOS camera. The LightCycler Nano Instrument is a 32-sample real-time PCR device for gene expression and genetic variation analyses in mono and dual color mode. Due to its small size, low weight and easy handling of reaction devices and software, this exceptionally silent instrument is ideal for scientists using real-time PCR for everyday use. The LightCycler Nano Instrument is designed for life science research only and not intended for use in diagnostic procedures.

The Applied Biosystems QuantStudio 7 Flex Real-Time PCR System advances your research further by ena (more)bling a broad range of real-time PCR-based applications through its multiplexing capabilities and interchangeable block formats. With a simplified workflow, intuitive software, automation capabilities, and touch-screen interface, the QuantStudio 7 Flex system offers exceptional reproducibility with minimal well-to-well and instrument-to-instrument variation. Features of the QuantStudio 7 Flex Real-Time PCR System include: Increased application versatility through the accomodation of 4 different block types Compatible with over 8 million TaqMan assays for gene expression, genetic variation, gene regulation, or protein expression experiments The intelligent design of the QuantStudio platform combined with Applied Biosystems instrument reliability and accuracy Easy-to-use software, responsive touch-screen, automation capabilities, and effortless block exchange without the need for any tools The QuantStudio 7 Flex system accommodates the interchange of a 96-well, 96-well Fast, 384-well, or TaqMan array card block. The system is delivered with one block type (a 96-well block in this case).

The Bax system is genetics based screening method that quickly and accurately detects target bacteri (more)a in raw ingredients, finished food products, environmental and water samples for fast accurate results. Through the polymerase chain reaction (PCR), the Bax system provides “yes-no” answers without the need for confirmationor expert interpretation of results. Tableted reagents provide minimal hands-on time using standard laboratory techniques in addition to accuracy, long shelf life and consistency. The Bax system cycler/detector provides even more ease of use through automated amplification and detection. Just load your samples and walk away. Electronic results that are clear, reliable and easily store are available in about four hours. Automated cycling, detection and analysis High capacity load of 96 samples per batch Single platform and protocol for all organisms allows testing for multiple targets in the same batch Electronic record of data for easy storage, retrieval and printing Pre-packaged PCR tablets have build-in control and ingredients to overcome PCR inhibition Excellent inclusivity and exclusivity for all targets No confirmation required Clear and reproducible results, independent of operator technique Minimal hands-on time using standard laboratory techniques Results as quickly as the day of sampling Performance meets or exceeds gel-based Bax systems Including software

BD MAX System is intended for in vitro diagnostic (IVD) use in performing FDA cleared or approved nu (more)cleic acid testing in clinical laboratories. It is capable of automated extraction and purification of nucleic acids from multiple specimen types, as well as the automated amplification and detection of target nucleic acid sequences by fluorescence-based PCR. The BD MAX System is capable of: Independent nucleic acid extraction and purification PCR amplification using thermal cycling across 24 lanes Real time detection of products of amplification Identification of each Sample Buffer tube by external barcode reader Work List verification by internal barcode reader Fluorescence detection at up to five wavelengths, allowing multiplex reactions by Cycle threshold (Ct) call, or multiplex reactions by melt analysis Qualitative, quantitative and melt sample analysis Features: Integrated and fully-automated extraction and thermocycling steps Processes and analyzes up to 24 specimens per run On-board lysis and extraction with pre-filled reagent strips Real-time PCR with two 24-lane microfluidic PCR readers Dedicated multi-color optics including: 475/520, 530/565, 585/630, 630/665, 680/715 Consistent results through standardized workflow and protocols Rapidly expanding menu and open-system options to help address emerging testing needs Less than one minute of hands on time per sample CLIA Moderately complex and easy to use All-in-One computer contains instrument software that is responsible for instrument control, self-calibration, data analysis and storage User interface can be navigated with point-and-click icons (keyboard and mouse), or the Computer’s touchscreen Modules included: Temperature control/heating subsystem to control thermal cycling A liquid handling head to transfer samples from Sample Buffer tubes to Unitized Reagent Strips Onboard readers to measure the amplification reaction and report results Software/user interface allows for: 24 unique thermal cycling channels per system (two onboard readers) Entering patient/sample information, either manually or by external barcode reader Monitoring operations, communication and storing of data Qualitative, quantitative and melt sample analysis Error warnings Download of results to a USB thumb drive LIS connectivity Dimensions (HxWxD): 940 x 755 x 725 mm Weight: 113 kg

The CFX96 Touch System is a powerful, precise, and flexible real-time PCR detection system. This six (more)-channel (five colors and one FRET channel) real-time PCR instrument combines advanced optical technology with precise temperature control to deliver sensitive, reliable detection for singlexplex or multiplex reactions. Quickly set up runs and monitor amplification traces in real time on the integrated LCD touch screen, or use the included CFX Maestro Software to easily and intuitively design your experiment and analyze results from a connected computer. With up to five-target detection, unsurpassed thermal cycler performance, unrivaled stand-alone functionality, and powerful yet easy-to-use software, the CFX96 Touch System is designed to advance your qPCR. Maximum ramp rate: 5°C/sec Average ramp rate: 3.3°C/sec Lid temperature: Heats up to 105°C Temperature range: 0 – 100°C Temperature accuracy (at 90°C): ±0.2°C Temperature uniformity (at 90°C): ±0.4°C, within 10 sec/well-to-well Gradient operational range: 30 – 100°C Gradient programmable span: 1 – 24°C Excitation: 6 filtered LEDs Detection: 6 filtered photodiodes Range of excitation/emission wavelengths: 450 – 730 nm Sensitivity: Detects 1 copy of target sequence in human genomic DNA Dynamic range: 10 orders of magnitude Scan time all channels: 12 sec Scan time FAM/SYBR Green only: 3 sec Sample size: 1 – 50 µl (10 – 25 µl recommended) Dimensions (WxDxH): 330 x 460 x 360 mm Weight: 21 kg

This Applied Biosystems ABI Prism 7700 Sequence Detection System  Model - 7700 was removed from (more) a university lab where it was surplus to requirements. The unit powers up but we were unable to test it further. It is in reasonable cosmetic condition but has marks from use. Pre-installation ManualPlanned Maintenance ProtocolSite Preparation & Safety Guide

Life Technologies Ion Torrent OneTouch 2 & ES System This was removed from a university lab whe (more)re it was surplus to requirements. The system powers up and is in good cosmetic condition with minor marks. Included are; Ion Torrent OneTouch 2Ref. INS1005527SN - 2456280-0958 Ion Torrent OneTouch ESModel - 844 1-21SN - 402237

Eppendorf Realplex 4 MasterCycler Epgradient S Real Time Cycler 96-Well This item was removed from a (more) university where it was surplus to requirement. It is in good cosmetic condition and powers on, however it does not include the required controller. We made several attempts to connect it to the software from another unit (not included), but it would not communicate Therefore, it is listed as Spares/Repair. Includes an Eppendorf USB drive with pre-installed software and a digital user manual.

Applied Biosciences 7900 HT Fast Real-Time PCR Complete System This was removed from a university l (more)ab where it was surplus to requirements. We were able to get the unit to communicate with the PC but we were unable to get it to run cycles due to a serial number conflict. We were able to open the door but not close it again. Included with it are; 1 x DELL PCWindows Vista Business 1 x SDS 2.3Item is in a good used condition. The Item powered on and working, but not connecting to PCR Tester 1 x Bti PRGF Desktop CentrifugeIt is in good used condition. The item powered on and was tested, all working 4 x PGRF CF10142 LOT 2894 1 x Diagnostica Stago PrinterItem powered on and tested, all working 1 x Datalogic QS6000 Plus Barcode ReaderThe item powered on and was tested, all working Associated books and a spare Block Assembly.

Applied Biosystems QuantStudio® 5 Real-Time PCR Instrument This item is sold on behalf of a client.  (more)It is surplus to requirements. It is in good cosmetic condition and in full working order. It is designed and calibrated for the 384-well block. Year of manufacture- Feb 2016 Last Preventative maintenance was carried out March 2024. It is available for collection from London UK, but we may be able to offer shipping for an additional fee. The normal terms and conditions do not apply to this item. This item is non returnable and non refundable.

This Agilent Stratagene Mx3005P Real-Time PCR System was removed from a university lab where it was  (more)surplus to requirements. The main unit is in reasonable used cosmetic condition but it does have some scrapes, scratches and marks from use. The unit powers up and both Green LED's light. You can also feel the well block heating up. The well block is 96 hole. The system includes:1 x Agilent Stratagene MX3005P. (various marks from use)1 x Dell E8400 PC with keyboard & mouse which we believe to be brand new.1 x iiyama monitor. (various marks from use) The PC has has a new HDD fitted and is running a fresh install of Windows 7. Also installed is a copy of Stratagene MxPro - Mx3005P v4.10 build 389. The software will control the Mx3005P unit. We have run a basic heat cycle with it and varified that the lamp will turn on & off. It has not been tested any further. The PC comes with a brand new sealed keyboard & mouse and a used iiyama monitor is also included. The system should include everything required to operate.

HOWE Refrigerated Solvent Trap   This was removed from a university lab where it was surplus to (more) requirements. The unit powers up and is in working order. It is in reasonable cosmetic condition with minor marks from use.

This Applied Biosystems 7900HT Fast Real-Time PCR System was removed from a university lab where it  (more)was surplus to requirements. The unit powers up but has not been tested further. It is in reasonably good cosmetic condition but has some marks from use.

Agilent Technologies Stratagene MX3000P System w/ Software Lab This system was removed from a univer (more)sity lab where it was surplus to requirement. It is in good cosmetic condition and powers on, it is paired with MxPro software. System software shows - Lamp Hours: 1823:49. PC runs Windows XP.

Applied Biosciences 7900 HT Fast Real-Time PCR Complete System Lab This item was removed from a univ (more)ersity where it was surplus to requirement. It is in good cosmetic condition and powers on, we are unable to test it further at our facility. Includes:- 16A 1Phase cable

This Applied Biosystems 7300 Real Time PCR System was removed from a university lab where it was sur (more)plus to requirements. The system is comprised of; 1 x Applied Biosystems 7300 Real-Time PCR Unit. 1 x Dell Optiplex 380 with Monitor, keyboard, mouse, USB cable & monitor cable.Running Applied Biosystems Sequence Detection Software (SDS)Version 1.2.3.The Dell has a Fresh install of Windows XP. The 7300 has had a function test run: USB - PassCCD - Pass (version 2.19)Filter Wheel - Pass (FW version G2.10)Shutter - Pass (FW version G2.10)Lamp - Pass (FW version G2.10)Thermal Cycler - Pass (G2.10 Firmware is up to date) The lamp status is Good.It has 1444 Usage hours.It was last replaced 08/01/2008. The unit will require the ROI calibration.

Corbett Research Rotor-Gene RG-6000 Real-time PCR Analyser This was removed from a university lab wh (more)ere it was surplus to requirements. The unit powers up and passes warmup sequences. It connects to the PC and communicates. It is fitted with a 100 disc with lockring. It is in very good cosmetic condition with minor marks. The system includes a Windows 7 Dell PC running Quaigen Rotor-Gene Q 2.4.3.

Corbett Research RG-3000 Rotor-Gene Real Time PCR Cycler Lab This item was removed from a university (more) where it was surplus to requirement. It is in good cosmetic condition and in good working order.

Bio-Rad MyIQ Single Colour Real-Time PCR Detection System iCycler & Software This was removed fr (more)om a university lab where it was surplus to requirements. The unit powers up and is in full working order. We connected it to the software and it all works well. It is in good cosmetic condition with minor marks. Included are; Bio-Rad MyIQ Single Colour Real-Time PCR Detection SystemModel - Bio-Rad MyiQ Optics ModuleSN - 569BR0869 Bio-Rad PTCModel - iCycler Thermal CyclerSN - 582BR01806296 Well Block holds 0.2ml tubes. Bio-Rad PC Has iQ5 Optical System Software Version 2.0 Monitor, Keyboard & Mouse Included.

This Applied Biosystems 7900HT Fast Real Time PCR System was removed from a university lab where it  (more)was surplus to requirements. The system powers up and is in good cosmetic condition with only minor usage marks. We have been able to connect the unit to the PC, but unable to test it further. It will likely require servicing before it is used. PC Equipment:-Dell Optiplex GX280 with Dell Monitor, keyboard and mousePSC QS6000 Plus Barcode Scanner PC has the following software:-Applied Biosystems 7900HT Version 2.3 Sequence Detection Systems (SDS)Applied Biosystems RQ Manager Version 1.2 External dimensions -D85 W54 H76cm

This Applied Biosystems 7900HT Fast Real Time PCR System was removed from a university lab where it  (more)was surplus to requirements. The system is in good used cosmetic condition. The lab confirmed it was in working order prior to removal. We have been able to connect the unit to the PC, but are unable to test it further. It consists of the following parts: 1 x Applied Biosystems 7900HTPart no. 4330966SN - 279002990 1 x SICK CLV422-1910S02 Fixed barcode scanner 1 x AB 7067/4 Microplate stacker. 1 x Symbol Barcode Scanner. 1 x Dell Optiplex 960 PC with Dell Monitor, keyboard and mouse. The has the following software installed:-Applied Biosystems 7900HT Version 2.4 Sequence Detection Systems (SDS)Applied Biosystems RQ Manager Version 1.2.1 We have all the passwords, which are available on request once the system has been sold.