Electroporation

The Nucleofector technology is a major improvement of the well known electroporation technology. The (more) Nucleofector technology involves a combination of optimized electrical parameters and specific Nucleofector solutions for different cell types. This combination permits the crucials step: delivery of the DNA of interest straight into the nucleus. As the DNA quickly reaches the nucleus, expression can begin without delay. The nucleofected cells are thus ready for analysis after a short incubation time of only 2 – 8 hours. DNA quality: 1 – 5 µg plasmid concentration per nuclefection sample (100 µl), plasmid concentration can be increased up to 10 µg per sample. This may lead to higher gene transfer efficiencies, but at the same time may increase mortality of the cells. Cell density: 2 x 105 – 1 x 107 cells per nuclefection sample for suspensions cells 2 x 105 – 2 x 106 cells per sample for adherent cells Cell densities of < 2 x 105 cells per smaple are not advisable beacause the gene transfer efficiency may decrease and mortality may increase. Dimensions (WxDxH): 300 x 230 x x110 mm Weight: 2.5 kg

The BTX HT-200 plate handler allows for the Highthroughput System HT 25 or HT 96 for advanced molecu (more)lar delivery. The HT 96 (96-well) plates or the HT 25 (25-well) plates can be electroporated using a BTX generator (models: ECM 830 and ECM 630) with a plate handler. The Model HT-200 automatically switches columns in a Multi-well plate during electroporation using the Auto-track sensing featute. The Auto-track sensing allows rapid electroporation of an entire plate by setting up all columns and pressing one button. Voltage Range: 0 to 3000V DC Pulse Length Range: 10 µsec to 10 sec Pulse Number Range: 1 to 99 Dimensions (WxDxH): 230 x 215 x 140 mm Weight: 4.8 kg Including: 1x 96-Well Disposable Electroporation Plates, 4 mm, 250 µl, pkg. of 1 1x 96-Well Disposable Electroporation Plates, 2 mm, 125 µl, pkg. of 1 1x HT 25-Well Adapter for Plate Handler

The Gemini X2 Systems is designed for researchers who need the ultimate experiment flexibility. In o (more)ne easy setup, square wave and exponential decay waves can be applied to cells in any format. These systems incorporate remote operation functionality via footswitch or PC and internal log storage of experiment data for easy optimization, quality control requirements and troubleshooting. Electroporation of suspension cells can be achieved in 96-well plates with the HT plate handler. Usable with all cell electroporation Features: Square Waveform, Multi-Pulsing Square Wave, Exponential Decay Waveform, Multi-Pulsing Exponential Decay, Resistance/Pulse Monitoring, Experiment Log Storage, Preprogrammed Protocols, Unlimited Custom Protocol Storage, Remote Operation, PC Communications Voltage Range: 5 to 3000 V Pulse Width Range: 10 μs to 5 s Pulse Interval: 100 ms to 30 s Dimensions (DxWxH): 324 x 286 x 216 mm Weight: 7.3 kg

This BTX Electro Square Porator ECM 830 was removed from a university lab where it was surplus to re (more)quirements. The item powers on and is in reasonably good cosmetic condition, we are unable to test it further at our facility.

Electroporator Lonza 4D-Nucleofactor System. Formed by two modules: unit Core (control unit) and un (more)it-X unit-transfection). Unit X performs 16-to 32-transfections per minute. Includes instruction manual and original packaging. 2014. Specifications: 240 V; 50-60 Hz The Core Unit - Controlling the 4D-Nucleofactor™ System The X unit – Supporting Nucleofection™ of various cell numbers in different formats